Purification and Some Properties of Sulfur Reductase from the Iron-Oxidizing Bacterium Thiobacillus ferrooxidans NASF-1.

2000 ◽  
Vol 90 (2) ◽  
pp. 199-203 ◽  
Author(s):  
KIM NG YONG ◽  
RYOKO SAWADA ◽  
SAYURI INOUE ◽  
KAZUO KAMIMURA ◽  
TSUYOSHI SUGIO
Keyword(s):  
Thiobacillus Ferrooxidans ◽  
Sulfur Reductase

Purification and some properties of sulfur reductase from the iron-oxidizing bacterium Thiobacillus ferrooxidans NASF-1

2000 ◽  
Vol 90 (2) ◽  
pp. 199-203 ◽  
Author(s):  
Kim Yong Ng ◽  
Ryoko Sawada ◽  
Sayuri Inoue ◽  
Kazuo Kamimura ◽  
Tsuyoshi Sugio
Keyword(s):  
Thiobacillus Ferrooxidans ◽  
Sulfur Reductase

Scanning Electron Microscope Study of Bacteria on Mineral Surfaces

1976 ◽  
Vol 34 ◽  
pp. 130-131
Author(s):  
V.K. Berry
Keyword(s):  
Oxidation Reaction ◽  
Electron Microscope Study ◽  
Elemental Sulfur ◽  
Thiobacillus Ferrooxidans ◽  
Physical Contact ◽  
Metal Sulfides ◽  
Low Grade ◽  
Mineral Surfaces ◽  
Mineral Surface ◽  
Scanning Electron Microscope Study

There are two strains of bacteria viz. Thiobacillus thiooxidansand Thiobacillus ferrooxidanswidely mentioned to play an important role in the leaching process of low-grade ores. Another strain used in this study is a thermophile and is designated Caldariella .These microorganisms are acidophilic chemosynthetic aerobic autotrophs and are capable of oxidizing many metal sulfides and elemental sulfur to sulfates and Fe2+ to Fe3+. The necessity of physical contact or attachment by bacteria to mineral surfaces during oxidation reaction has not been fairly established so far. Temple and Koehler reported that during oxidation of marcasite T. thiooxidanswere found concentrated on mineral surface. Schaeffer, et al. demonstrated that physical contact or attachment is essential for oxidation of sulfur.

Laser-guided pattern writing through Thiobacillus ferrooxidans metabolite

2009 ◽  
Vol 86 (4-6) ◽  
pp. 565-568 ◽  
Author(s):  
H. Hocheng ◽  
J.C. Wang ◽  
J.H. Chang ◽  
W.C. Shen
Keyword(s):  
Thiobacillus Ferrooxidans

FORMATION OF POLYTHIONATES AND THEIR INTERRELATIONSHIPS DURING OXIDATION OF THIOSULFATE BY THIOBACILLUS FERROOXIDANS

1966 ◽  
Vol 12 (5) ◽  
pp. 1041-1054 ◽  
Author(s):  
D. B. Sinha ◽  
C. C. Walden
Keyword(s):  
Metabolic Pathway ◽  
Thiobacillus Ferrooxidans ◽  
Chemical Interaction ◽  
Major Pathway

The metabolic pathway for the utilization of thiosulfate by the acidophilic chemoautotroph, Thiobacillus ferrooxidans, has been examined by chromatographic and radiographic techniques. With suitable chemical controls, it was found that the major pathway involved 2 moles of thiosulfate passing to tetrathionate to trithionate regenerating 1 mole of thiosulfate concurrent with the production of 2 moles of sulfate. Thus, previous findings which demonstrate this pathway as far as trithionate, made with other thiobacilli, are sound despite recent documentation of the chemical interaction of various polythionates. The cyclical pathway, proposed here, explains some findings of other workers.

Temperature studies of iron-oxidizing autotrophs and acidophilic heterotrophs isolated from uranium mines

1993 ◽  
Vol 39 (4) ◽  
pp. 384-388 ◽  
Author(s):  
Deborah Berthelot ◽  
L. G. Leduc ◽  
G. D. Ferroni
Keyword(s):  
Water Samples ◽  
Mine Water ◽  
Thiobacillus Ferrooxidans ◽  
Incubation Temperature ◽  
Temperature Growth ◽  
Temperature Range ◽  
Uranium Mines ◽  
Iron Oxidizing Bacteria ◽  
Colony Forming Units ◽  
Growth Profiles

Iron-oxidizing autotrophs and acidophilic heterotrophs were quantified at an incubation temperature of 18 °C in several samples obtained from the bioleaching areas of two uranium mines in Ontario, Canada. All samples were mine-water samples with temperatures in the range 13–18 °C. Iron-oxidizing autotrophs ranged from 2683 ± 377 to 245 000 ± 20 205 colony-forming units∙mL−1 and were always numerically superior to acidophilic heterotrophs, which ranged from 40 ± 8 to 9650 ± 161 colony-forming units∙mL−1. For each sample, approximately 20 isolates of each nutritional group were examined for the ability to grow at temperatures of 4, 18, 21, and 37 °C, respectively; overall, 559 isolates of iron-oxidizing bacteria (predominantly Thiobacillus ferrooxidans) and 252 acidophilic heterotrophic isolates were examined and categorized as 'broader temperature range psychrotrophs,' 'narrower temperature range psychrotrophs,' 'intermediates,' or mesophiles. Although psychrotrophic representatives of both groups were abundant, no psychrophiles were recovered from any of the samples. For the iron oxidizers, the temperature growth profiles of the isolates were similar from sample to sample. For the acidophilic heterotrophs, the temperature growth profiles varied considerably among samples.Key words: psychrotrophs; Thiobacillus ferrooxidans; uranium mines.

Nucleotide sequence and expression of a cloned Thiobacillus ferrooxidans recA gene in Escherichia coli

Gene ◽  
1989 ◽  
Vol 78 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Rajkumar S. Ramesar ◽  
Valerie Abratt ◽  
David R. Woods ◽  
Douglas E. Rawlings
Keyword(s):  
Escherichia Coli ◽  
Nucleotide Sequence ◽  
Thiobacillus Ferrooxidans ◽  
Reca Gene

Leaching of chalcopyrite concentrates with thiobacillus ferrooxidans in batch culture

1976 ◽  
Vol 2 (2) ◽  
pp. 87-103 ◽  
Author(s):  
A. Pinches ◽  
F.O. Al-Jaid ◽  
D.J.A. Williams ◽  
B. Atkinson
Keyword(s):  
Batch Culture ◽  
Thiobacillus Ferrooxidans
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