Overexpression of GADD34 Enhances Production of Recombinant Human Antithrombin III in Chinese Hamster Ovary Cells

2008 ◽  
Vol 106 (6) ◽  
pp. 568-573 ◽  
Author(s):  
Takeshi Omasa ◽  
Takashi Takami ◽  
Tomoshi Ohya ◽  
Eriko Kiyama ◽  
Tetsuji Hayashi ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Antithrombin Iii ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Improved production of recombinant human antithrombin III in Chinese hamster ovary cells by ATF4 overexpression

2008 ◽  
Vol 100 (2) ◽  
pp. 317-324 ◽  
Author(s):  
Tomoshi Ohya ◽  
Tetsuji Hayashi ◽  
Eriko Kiyama ◽  
Hiroko Nishii ◽  
Hideo Miki ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Antithrombin Iii ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Decrease in Antithrombin III Fucosylation by Expressing GDP-fucose Transporter siRNA in Chinese Hamster Ovary Cells

2008 ◽  
Vol 106 (4) ◽  
pp. 417
Author(s):  
Takeshi Omasa ◽  
Ryo Tanaka ◽  
Tomohiro Doi ◽  
Masayuki Ando ◽  
Yuka Kitamoto ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Antithrombin Iii ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Decrease in antithrombin III fucosylation by expressing GDP-fucose transporter siRNA in Chinese hamster ovary cells

2008 ◽  
Vol 106 (2) ◽  
pp. 168-173 ◽  
Author(s):  
Takeshi Omasa ◽  
Ryo Tanaka ◽  
Tomohiro Doi ◽  
Masayuki Ando ◽  
Yuka Kitamoto ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Antithrombin Iii ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells

Recombinant Human Soluble Thrombomodulin Delivers Bounded Thrombin to Antithrombin III: Thrombomodulin Associates with Free Thrombin and Is Recycled to Activate Protein C

1993 ◽  
Vol 70 (03) ◽  
pp. 418-422 ◽  
Author(s):  
Masaharu Aritomi ◽  
Naoko Watanabe ◽  
Rika Ohishi ◽  
Komakazu Gomi ◽  
Takao Kiyota ◽  
...  
Keyword(s):  
Protein C ◽  
Antithrombin Iii ◽  
Transmembrane Domain ◽  
Chinese Hamster Ovary Cells ◽  
Time Lag ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Soluble Thrombomodulin ◽  
Simulation Based ◽  
Recombinant Human Soluble Thrombomodulin

SummaryRecombinant human soluble thrombomodulin (rhs-TM), having no transmembrane domain or chondroitin sulfate, was expressed in Chinese hamster ovary cells. Interactions between rhs-TM, thrombin (Th), protein C (PC) and antithrombin III (ATIII) were studied. Equilibrium between rhs-TM and Th had no detectable time lag in clotting inhibition (K d = 26 nM) or PC activation (K d = 22 nM), while ATIII inhibited Th at a bimolecular rate constant = 5,200 M-1s-1 (K d <0.2 nM). A mixture of ATIII, Th and rhs-TM showed that ATIII reacted with Th slower than rhs-TM, whose presence did not affect the reaction between ATIII and Th. In a mixture of rhs-TM, ATIII and PC, the repeated addition of Th caused the repeated activation of PC; which was consistent with the Simulation based on the assumption that rhs-TM is recycled as a Th cofactor. From these results, we concluded that upon inhibition of the rhs-TM-Th complex by ATIII, rhs-TM is released to recombine with free Th and begins to activate PC, while the Th-ATIII complex does not affect rhs-TM-Th equilibrium.

Malignancy-related characteristics of wild type and drug-resistant chinese hamster ovary cells

Pathology ◽  
1993 ◽  
Vol 25 (3) ◽  
pp. 268-276 ◽  
Author(s):  
Wanda B. Mackinnon ◽  
Marlen Dyne ◽  
Rebecca Hancock ◽  
Carolyn E. Mountford ◽  
Adrienne J. Grant ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Drug Resistant ◽  
Wild Type ◽  
Ovary Cells
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