Expression of the Bacterial Hemoglobin Gene from Vitreoscilla stercoraria Increases Rifamycin B Production in Amycolatopsis mediterranei

2008 ◽  
Vol 106 (5) ◽  
pp. 493-497 ◽  
Author(s):  
Guerra Priscila ◽  
Francisco J. Fernández ◽  
Angel E. Absalón ◽  
Ma. Del Rocío Suarez ◽  
Mara Sainoz ◽  
...  
Keyword(s):  
Amycolatopsis Mediterranei ◽  
Bacterial Hemoglobin ◽  
Hemoglobin Gene ◽  
Rifamycin B

Effect of Variation in Copper Sources and Growth Conditions on the Copper Uptake by Bacterial Hemoglobin Gene (vgb) Bearing E. coli

2006 ◽  
Vol 9 (11) ◽  
pp. 2022-2031 ◽  
Author(s):  
Khaled M. Khleifa ◽  
Muayad Abboud . ◽  
Mohammad Laymun . ◽  
Khalid Al-Sharafa . ◽  
Khaled Tarawneh .
Keyword(s):  
Growth Conditions ◽  
Copper Uptake ◽  
E Coli ◽  
Bacterial Hemoglobin ◽  
Hemoglobin Gene

scholarly journals Overproduction of Rifamycin B by Amycolatopsis mediterranei and Its Relationship with the Toxic Effect of Barbital on Growth.

1998 ◽  
Vol 51 (1) ◽  
pp. 58-63 ◽  
Author(s):  
ARMANDO MEJÍA ◽  
JAVIER BARRIOS-GONZÁLEZ ◽  
GUSTAVO VINIEGRA-GONZÁLEZ
Keyword(s):  
Toxic Effect ◽  
Amycolatopsis Mediterranei ◽  
Rifamycin B

scholarly journals Rifamycin W Analogues from Amycolatopsis mediterranei S699 Δrif-orf5 Strain

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 920
Author(s):  
Yanrong Shi ◽  
Feng Ye ◽  
Yuliang Song ◽  
Xiaochun Zhang ◽  
Chunhua Lu ◽  
...  
Keyword(s):  
Mass Spectra ◽  
Inhibitory Concentration ◽  
2D Nmr ◽  
Oxidative Cleavage ◽  
Amycolatopsis Mediterranei ◽  
P450 Monooxygenase ◽  
Extensive Analysis ◽  
Ic50 Values ◽  
Orf5 Gene ◽  
Rifamycin B

Rifamycin W, the most predominant intermediate in the biosynthesis of rifamycin, needs to undergo polyketide backbone rearrangement to produce rifamycin B via an oxidative cleavage of the C-12/C-29 double bond. However, the mechanism of this putative oxidative cleavage has not been characterized yet. Rif-Orf5 (a putative cytochrome P450 monooxygenase) was proposed to be involved in the cleavage of this olefinic moiety of rifamycin W. In this study, the mutant strain Amycolatopsis mediterranei S699 Δrif-orf5 was constructed by in-frame deleting the rif-orf5 gene to afford thirteen rifamycin W congeners (1–13) including seven new ones (1–7). Their structures were elucidated by extensive analysis of 1D and 2D NMR spectroscopic data and high-resolution ESI mass spectra. Presumably, compounds 1–4 were derivatized from rifamycin W via C-5/C-11 retro-Claisen cleavage, and compounds 1–3, 9 and 10 featured a hemiacetal. Compounds 5–7 and 11 showed oxygenations at various sites of the ansa chain. In addition, compounds 1–3 exhibited antibacterial activity against Staphylococcus aureus with minimal inhibitory concentration (MIC) values of 5, 40 and 0.5 µg/mL, respectively. Compounds 1 and 3 showed modest antiproliferative activity against HeLa and Caco-2 cells with half maximal inhibitory concentration (IC50) values of about 50 µM.

Differential mass spectrometry-based proteome analyses unveil major regulatory hubs in rifamycin B production in Amycolatopsis mediterranei

2021 ◽  
pp. 104168
Author(s):  
Nirjara Singhvi ◽  
Priya Singh ◽  
Om Prakash ◽  
Vipin Gupta ◽  
Sukanya Lal ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amycolatopsis Mediterranei ◽  
Rifamycin B

Evaluation of Amycolatopsis mediterranei VA18 for production of rifamycin-B

2000 ◽  
Vol 36 (4) ◽  
pp. 305-309 ◽  
Author(s):  
G Venkateswarlu ◽  
P.S Murali Krishna ◽  
Ashok Pandey ◽  
L Venkateshwar Rao
Keyword(s):  
Amycolatopsis Mediterranei ◽  
Rifamycin B
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