Improving enzyme immobilization in mesocellular siliceous foams by microwave irradiation

2008 ◽  
Vol 106 (3) ◽  
pp. 286-291 ◽  
Author(s):  
Anming Wang ◽  
Mingqing Liu ◽  
Hua Wang ◽  
Cheng Zhou ◽  
Zhiqiang Du ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Enzyme Immobilization ◽  
Mesocellular Siliceous Foams

scholarly journals Stable and efficient immobilization of bi-enzymatic NADPH cofactor recycling system under consecutive microwave irradiation

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242564
Author(s):  
Rong Chen ◽  
Qiuhui Wei ◽  
Xin Wei ◽  
Yuheng Liu ◽  
Xiaomin Zhang ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Redox Reactions ◽  
Glucose Dehydrogenase ◽  
Relative Activity ◽  
Efficient Catalyst ◽  
Recycling System ◽  
Cofactor Recycling ◽  
Industrial Preparation ◽  
Enzymatic Cascades ◽  
Mesocellular Siliceous Foams

One of the challenges in biocatalysis is the development of stable and efficient bi-enzymatic cascades for bio-redox reactions coupled to the recycling of soluble cofactors. Aldo-keto reductase (LEK) and glucose dehydrogenase (GDH) can be utilized as the NADPH recycling system for economic and efficient biocatalysis of (R)-4-chloro-3-hydroxybutanoate ((R)-CHBE), an important chiral pharmaceutical intermediate. The LEK and GDH was efficiently co-immobilized in mesocellular siliceous foams (MCFs) under microwave irradiation (CoLG-MIA). while they were also co-immobilized by entrapment in calcium alginate without MIA as control (CoLG-CA). The relative activity of CoLG-MIA was increased to 140% compared with that of free LEK. The CoLG-MIA exhibited a wider range of pH and temperature stabilities compared with other preparations. The thermal, storage and batch operational stabilities of microwave-assisted immobilized LEK-GDH were also improved. The NADPH recycling system exhibited the potential as the stable and efficient catalyst for the industrial preparation of (R)-CHBE.

Environmentally Friendly Preparation of Magnetic Composites Featuring Proteolytic Properties

INEOS OPEN ◽  
2020 ◽  
Author(s):  
N. A. Samoilova ◽  
Keyword(s):  
Enzyme Immobilization ◽  
Maleic Acid ◽  
Environmentally Friendly ◽  
Synthetic Polymer ◽  
Magnetic Composites ◽  
Interpolyelectrolyte Complex ◽  
Hydrolysis Rates ◽  
Poly Ethylene ◽  
Noncovalent Binding

The enzyme-containing magnetic composites are presented. The magnetic matrix for enzyme immobilization is obtained by sequential application of an amine-containing polysaccharide—chitosan and a synthetic polymer—poly(ethylene-alt-maleic acid) to the magnetite microparticles to form the interpolyelectrolyte complex shell. Then, the enzyme (trypsin) is immobilized by covalent or noncovalent binding. Thus, the suggested composites can be readily obtained in the environmentally friendly manner. The enzyme capacity of the resulting composites reaches 28.0–32.6 mg/g. The maximum hydrolysis rates of the H-Val-Leu-Lys-pNA substrate provided by these composites range within 0.60·10–7–0.77·10–7 M/min.

Eco-Friendly Synthesis of Pyrazoline Derivatives

2017 ◽  
Vol 9 (04) ◽  
Author(s):  
Mahesh G. Kharatmol ◽  
Deepali Jagdale
Keyword(s):  
Ionic Liquids ◽  
Microwave Irradiation ◽  
Organic Synthesis ◽  
Ultrasonic Irradiation ◽  
Minimal Energy ◽  
Anticancer Activities ◽  
Conventional Methods

Pyrazoline class of compounds serve as better moieties for an array of treatments, they have antibacterial, antifungal, antiinflammatory, antipyretic, diuretic, cardiovascular activities. Apart from these they also have anticancer activities. So, pertaining to its importance, many attempts are made to synthesize pyrazolines. Since conventional methods of organic synthesis are energy and time consuming. There are elaborate pathways for green and eco-friendly synthesis of pyrazoline derivatives including microwave irradiation, ultrasonic irradiation, grinding and use of ionic liquids which assures the synthesis of the same within much lesser time and by use of minimal energy

Biocatalytic Ketone Reductions Using Biobeads

2020 ◽  
Author(s):  
Jia Shen Chew ◽  
Ken Chi Lik Lee ◽  
THI THANH NHA HO
Keyword(s):  
Enzyme Immobilization ◽  
High Throughput ◽  
High Throughput Screening ◽  
Chiral Hplc ◽  
Micro Scale

<p>Lee and coworkers offers a kind of new concept to enzyme immobilization and explores its suitability in the context of miniaturisation and high-throughput screening. Here, polystyrene-immobilized ketoreductases are compared with its non-immobilized counterparts in terms of conversion and stereoselectivity (both determined by chiral HPLC), and the study indicates that the BioBeads perform similarly (sometimes slightly more selective) which may be useful whenever defined micro-scale amounts of biocatalysts were required in high-throughput experiment settings.</p>

Growth Process of SiC Grains Prepared by High-energy Microwave Irradiation

2014 ◽  
Vol 29 (2) ◽  
pp. 149-154 ◽  
Author(s):  
Shan HUANG ◽  
Ji-Gang WANG ◽  
Song LIU ◽  
Fan LI
Keyword(s):  
Microwave Irradiation ◽  
Growth Process ◽  
High Energy

Atom Transfer Radical Copolymerization of Acrylamide Under Microwave Irradiation

2013 ◽  
Vol 30 (4) ◽  
pp. 398
Author(s):  
Wei DING ◽  
Ling WANG ◽  
Tao YU ◽  
Guangmiao QU ◽  
Xiang GAO ◽  
...  
Keyword(s):  
Microwave Irradiation ◽  
Radical Copolymerization ◽  
Atom Transfer
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