Translational enhancement of recombinant protein synthesis in transgenic silkworms by a 5′-untranslated region of polyhedrin gene of Bombyx mori Nucleopolyhedrovirus

2008 ◽  
Vol 105 (6) ◽  
pp. 595-603 ◽  
Author(s):  
Masashi Iizuka ◽  
Masahiro Tomita ◽  
Katsuhiko Shimizu ◽  
Yutaka Kikuchi ◽  
Katsutoshi Yoshizato
Keyword(s):  
Protein Synthesis ◽  
Recombinant Protein ◽  
Bombyx Mori ◽  
Untranslated Region ◽  
Polyhedrin Gene ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Transgenic Silkworms

Resistance to Bombyx mori nucleopolyhedrovirus via overexpression of an endogenous antiviral gene in transgenic silkworms

2012 ◽  
Vol 157 (7) ◽  
pp. 1323-1328 ◽  
Author(s):  
Liang Jiang ◽  
Genhong Wang ◽  
Tingcai Cheng ◽  
Qiong Yang ◽  
Shengkai Jin ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Transgenic Silkworms ◽  
Antiviral Gene

scholarly journals Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus

2017 ◽  
Vol 91 (8) ◽  
Author(s):  
Shuqing Chen ◽  
Chengxiang Hou ◽  
Honglun Bi ◽  
Yueqiang Wang ◽  
Jun Xu ◽  
...  
Keyword(s):  
Antiviral Therapy ◽  
Bombyx Mori ◽  
Transgenic Animals ◽  
Viral Gene ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Guide Rnas ◽  
Virus Clearance ◽  
Short Palindromic Repeat ◽  
Transgenic Silkworms ◽  
Pathogen Genome

ABSTRACT We developed a novel antiviral strategy by combining transposon-based transgenesis and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system for the direct cleavage of Bombyx mori nucleopolyhedrovirus (BmNPV) genome DNA to promote virus clearance in silkworms. We demonstrate that transgenic silkworms constitutively expressing Cas9 and guide RNAs targeting the BmNPV immediate early-1 (ie-1) and me53 genes effectively induce target-specific cleavage and subsequent mutagenesis, especially large (∼7-kbp) segment deletions in BmNPV genomes, and thus exhibit robust suppression of BmNPV proliferation. Transgenic animals exhibited higher and inheritable resistance to BmNPV infection than wild-type animals. Our approach will not only contribute to modern sericulture but also shed light on future antiviral therapy. IMPORTANCE Pathogen genome targeting has shown its potential in antiviral research. However, transgenic CRISPR/Cas9 system-mediated viral genome targeting has not been reported as an antiviral strategy in a natural animal host of a virus. Our data provide an effective approach against BmNPV infection in a real-world biological system and demonstrate the potential of transgenic CRISPR/Cas9 systems in antiviral research in other species.

scholarly journals Destruxin A Interacts with Aminoacyl tRNA Synthases in Bombyx mori

2021 ◽  
Vol 7 (8) ◽  
pp. 593
Author(s):  
Jingjing Wang ◽  
Alexander Berestetskiy ◽  
Qiongbo Hu
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Bombyx Mori ◽  
Metarhizium Anisopliae ◽  
Free Amino ◽  
Molecular Target ◽  
Trna Synthetases ◽  
Interaction Mode ◽  
Aminoacyl Trna Synthetases ◽  
Wide Range

Destruxin A (DA), a hexa-cyclodepsipeptidic mycotoxin produced by the entomopathogenic fungus Metarhizium anisopliae, exhibits insecticidal activities in a wide range of pests and is known as an innate immunity inhibitor. However, its mechanism of action requires further investigation. In this research, the interactions of DA with the six aminoacyl tRNA synthetases (ARSs) of Bombyx mori, BmAlaRS, BmCysRS, BmMetRS, BmValRS, BmIleRS, and BmGluProRS, were analyzed. The six ARSs were expressed and purified. The BLI (biolayer interferometry) results indicated that DA binds these ARSs with the affinity indices (KD) of 10−4 to 10−5 M. The molecular docking suggested a similar interaction mode of DA with ARSs, whereby DA settled into a pocket through hydrogen bonds with Asn, Arg, His, Lys, and Tyr of ARSs. Furthermore, DA treatments decreased the contents of soluble protein and free amino acids in Bm12 cells, which suggested that DA impedes protein synthesis. Lastly, the ARSs in Bm12 cells were all downregulated by DA stress. This study sheds light on exploring and answering the molecular target of DA against target insects.

scholarly journals Dynamic Interactions between Bombyx mori Nucleopolyhedrovirus and Its Host Cells Revealed by Transcriptome Analysis

2012 ◽  
Vol 86 (13) ◽  
pp. 7345-7359 ◽  
Author(s):  
J. Xue ◽  
N. Qiao ◽  
W. Zhang ◽  
R.-L. Cheng ◽  
X.-Q. Zhang ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Transcriptome Analysis ◽  
Host Cells ◽  
Bombyx Mori Nucleopolyhedrovirus ◽  
Dynamic Interactions

Bombyx mori transferrin: Genomic structure, expression and antimicrobial activity of recombinant protein

2009 ◽  
Vol 33 (10) ◽  
pp. 1064-1069 ◽  
Author(s):  
Eun-Young Yun ◽  
Jae-Keum Lee ◽  
O-Yu Kwon ◽  
Jae-Sam Hwang ◽  
Iksoo Kim ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Recombinant Protein ◽  
Bombyx Mori ◽  
Genomic Structure
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