Exogenous Hormones Affecting Morphology and Biosynthetic Potential of Hairy Root Line (LYR2i) of Linum album

2008 ◽  
Vol 105 (2) ◽  
pp. 140-146 ◽  
Author(s):  
Sunita Farkya ◽  
Virendra Swarup Bisaria
Keyword(s):  
Hairy Root ◽  
Exogenous Hormones ◽  
Linum Album ◽  
Hairy Root Line

High Podophyllotoxin Producing Hairy Root Line of Linum Album: Influence of Carbohydrates, Temperature and Photoperiod

2013 ◽  
Vol 2 (2) ◽  
pp. 134-141 ◽  
Author(s):  
Sunita Farkya ◽  
Gaurav Rajouria ◽  
Vinod Kumar ◽  
Virendra Bisaria
Keyword(s):  
Hairy Root ◽  
Linum Album ◽  
Hairy Root Line

scholarly journals Study on the growth of Agrobacterium rhizogenes-induced Catharanthus roseus hairy roots in liquid media

2019 ◽  
Vol 2 (1) ◽  
pp. 5-12
Author(s):  
Nguyen Nhu Nhut ◽  
Bui Van Le
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Catharanthus Roseus ◽  
Growth Kinetics ◽  
Exponential Phase ◽  
Liquid Media ◽  
Hairy Root Line ◽  
Initial Ph ◽  
Initial Results

Catharanthus roseus hairy roots induced by Agrobacterium rhizogenes get potential to apply to many fields. The suitable conditions for culturing depend on each hairy root line. In shaken liquid media in dark at 25 oC, VIN002-12 and VIN005-07 hairy root lines had the best growth in hemi-concentrated Gamborg’B5 media while VIN072-15 and VIN077-09 lines showed the best growth in in hemi-concentrated White media. The appropriate initial pH of medium for the lines was in range of 5.7–6.5. The hairy root lines used 2–5% sucrose as an optimal source of carbon for their growth. In selected conditions, the growth kinetics curves showed the end of exponential phase at the 28th day of culture with VIN005-07 and VIN072- 15 lines whereas at the 35th day with VIN002-12 and VIN077-09 lines. The initial results are quite possible to produce biomass for researches on four hairy root lines in the future.  

Establishment of Salvia officinalis L. Hairy Root Cultures for the Production of Rosmarinic Acid

2006 ◽  
Vol 61 (5-6) ◽  
pp. 351-356 ◽  
Author(s):  
Izabela Grzegorczyk ◽  
Aleksandra Królicka ◽  
Halina Wysokińska
Keyword(s):  
Hairy Root ◽  
Hairy Roots ◽  
Rosmarinic Acid ◽  
Root Culture ◽  
Dry Weight ◽  
Salvia Officinalis ◽  
Root Cultures ◽  
Hairy Root Line ◽  
Salvia Officinalis L ◽  
Agrobacterium Rhizogenes Strains

Shoots of Salvia officinalis, a medicinally important plant, were infected with Agrobacterium rhizogenes strains ATCC 15834 and A4 which led to the induction of hairy roots in 57% and 37% of the explants, respectively. Seven lines of hairy roots were established in WP liquid medium under light and dark conditions. The transformed nature of the root lines was confirmed by polymerase chain reaction using rolB and rolC specific primers. Transformed root cultures of Salvia officinalis showed variations in biomass and rosmarinic acid production depending on the bacterial strain used for transformation and the root line analyzed. Both parameters (growth and rosmarinic acid content) of ATCC 15834-induced lines were significantly higher than the A4-induced lines. The maximum accumulation of rosmarinic acid (about 45 mg g-1 of dry weight) was achieved by hairy root line 1 (HR-1) at the end of the culture period (45D50 days). The level was significantly higher than that found in untransformed root culture (19 mg g-1 of dry wt).

Significant enhancement of lignan accumulation in hairy root cultures of Linum album using biotic elicitors

2014 ◽  
Vol 36 (12) ◽  
pp. 3325-3331 ◽  
Author(s):  
Sedigheh Esmaeilzadeh Bahabadi ◽  
Mozafar Sharifi ◽  
Najme Ahmadian Chashmi ◽  
Jun Murata ◽  
Honoo Satake
Keyword(s):  
Hairy Root ◽  
Significant Enhancement ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Linum Album

scholarly journals Response Surface Modelling of Noradrenaline Production in Hairy Root Culture of Purslane (Portulaca oleracea L.)

2015 ◽  
Vol 3 (6) ◽  
pp. 349 ◽  
Author(s):  
Mehdi Ghorbani ◽  
Aghil Ghorbani ◽  
Mansoor Omidi ◽  
Seyed Mohammad Hashemi
Keyword(s):  
Response Surface ◽  
Hairy Root ◽  
Root Culture ◽  
Dry Weight ◽  
Hairy Root Culture ◽  
Portulaca Oleracea ◽  
Noradrenaline Content ◽  
Hairy Root Line ◽  
Response Surface Modelling ◽  
Common Purslane

Common purslane (Portulaca oleracea L.) is an annual plant as one of the natural sources for noradrenaline hormone. In this research, hairy root culture of purslane was established by using Agrobacterium rhizogenes strain ATCC 15834. In the following, Box-Behnken model of response surface methodology (RSM) was employed to optimize B5 medium for the growth of P. oleracea L. hairy root line. According to the results, modelling and optimization conditions, including sucrose, CaCl2.H2O, H2PO4 and NO3-/NH4+ concentrations on maximum dry weight (0.155 g) and noradrenaline content (0.36 mg.g-1 DW) was predicted. These optimal conditions predicted by RSM were confirmed the enhancement of noradrenaline production as an application potential for production by hairy root cultures.

scholarly journals Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss

2012 ◽  
Vol 22 (2) ◽  
pp. 745-752 ◽  
Author(s):  
Najmeh Ahmadian Chashmi ◽  
Mozafar Sharifi ◽  
Morteza Yousefzadi ◽  
Mehrdad Behmanesh ◽  
Hassan Rezadoost ◽  
...  
Keyword(s):  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Linum Album
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