Two-Dimensional DNA Gel Electrophoresis Mapping: a Novel Approach to Diversity Analysis of Bacterial Communities in Environmental Soil

2008 ◽  
Vol 105 (2) ◽  
pp. 127-133 ◽  
Author(s):  
Guohua Liu ◽  
Takashi Amemiya ◽  
Kiminori Itoh
Keyword(s):  
Bacterial Communities ◽  
Gel Electrophoresis ◽  
Diversity Analysis ◽  
Two Dimensional ◽  
Novel Approach

Direct end labelling of telomeres

Genome ◽  
1993 ◽  
Vol 36 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Alexander Kolchinsky ◽  
Peter M. Gresshoff
Keyword(s):  
Glycine Max ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Telomeric Sequence ◽  
Two Dimensional ◽  
Pulsed Field ◽  
Two Dimensional Electrophoresis ◽  
Restriction Nuclease ◽  
Novel Approach ◽  
Dimensional Electrophoresis

A novel approach of direct end labelling of telomeres is presented. Chromosome-sized, agarose-embedded DNA was treated with T4 DNA polymerase to remove protruding 3′ end of telomeres and to generate single-stranded 5′ ends. The DNA was then labelled by the same enzyme in the presence of [α-32P|dGTP and cold dATP and dTTP. Labelled yeast chromosomes separated by pulsed field gel electrophoresis maintained their integrity. Digestion of yeast chromosomes separated in pulsed field gels with a restriction nuclease (HinfI), followed by conventional electrophoresis in the second dimension, resulted in a fingerprint-like pattern of labelled telomeres. This was very similar to the hybridization pattern of a similar two-dimensional gel probed with cloned yeast telomeric sequence. The same approach enabled us to label telomeres in soybean, determine their size, and to reveal polymorphisms in the length of telomeres between the closely related subspecies Glycine max (soybean) and Glycine soja.Key words: telomeres, Saccharomyces cerevisiae, Glycine max, two-dimensional electrophoresis, DNA polymorphism.

Diversity of Glycoprotein Deficiencies in Glanzmann’s Thrombasthenia

1985 ◽  
Vol 54 (03) ◽  
pp. 626-629 ◽  
Author(s):  
M Meyer ◽  
F H Herrmann
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Type Ii ◽  
Two Dimensional ◽  
Two Dimensional Gel Electrophoresis ◽  
Structural Variant ◽  
Glanzmann’S Thrombasthenia ◽  
Crossed Immunoelectrophoresis ◽  
Glanzmann's Thrombasthenia ◽  
Platelet Proteins

SummaryThe platelet proteins of 9 thrombasthenic patients from 7 families were analysed by high resolution two-dimensional gel electrophoresis (HR-2DE) and crossed immunoelectrophoresis (CIE). In 7 patients both glycoproteins (GPs) IIb and Ilia were absent or reduced to roughly the same extent. In two related patients only a trace of GP Ilb-IIIa complex was detected in CIE, but HR-2DE revealed a glycopeptide in the position of GP Ilia in an amount comparable to type II thrombasthenia. This GP Ilia-like component was neither recognized normally by anti-GP Ilb-IIIa antibodies nor labeled by surface iodination. In unreduced-reduced two-dimensional gel electrophoresis two components were observed in the region of GP Ilia. The assumption of a structural variant of GP Ilia in the two related patients is discussed.

Comparative Two-Dimensional Gel Electrophoresis of Trypanosoma cruzi Mammalian-Stage Forms in an Alkaline pH Range

2015 ◽  
Vol 22 (12) ◽  
pp. 1066-1075 ◽  
Author(s):  
Adriana Magalhães ◽  
Rayner Queiroz ◽  
Izabela Bastos ◽  
Jaime Santana ◽  
Marcelo Sousa ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Alkaline Ph ◽  
Two Dimensional Gel Electrophoresis ◽  
Ph Range
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