Bacillus subtilis AprX involved in degradation of a heterologous protein during the late stationary growth phase

2007 ◽  
Vol 104 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Takeko Kodama ◽  
Keiji Endo ◽  
Kazuhisa Sawada ◽  
Katsutoshi Ara ◽  
Katsuya Ozaki ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Growth Phase ◽  
Heterologous Protein ◽  
Stationary Growth Phase ◽  
Stationary Growth

scholarly journals Temporal Expression of the Bacillus subtilis secAGene, Encoding a Central Component of the Preprotein Translocase

1999 ◽  
Vol 181 (2) ◽  
pp. 493-500 ◽  
Author(s):  
Markus Herbort ◽  
Michael Klein ◽  
Erik H. Manting ◽  
Arnold J. M. Driessen ◽  
Roland Freudl
Keyword(s):  
Bacillus Subtilis ◽  
Growth Phase ◽  
Exponential Growth ◽  
De Novo ◽  
Stationary Growth Phase ◽  
Extracellular Proteins ◽  
Exponential Growth Phase ◽  
Central Component ◽  
Temporal Expression ◽  
Stationary Growth

ABSTRACT In Bacillus subtilis, the secretion of extracellular proteins strongly increases upon transition from exponential growth to the stationary growth phase. It is not known whether the amounts of some or all components of the protein translocation apparatus are concomitantly increased in relation to the increased export activity. In this study, we analyzed the transcriptional organization and temporal expression of the secA gene, encoding a central component of the B. subtilis preprotein translocase. We found that secA and the downstream gene (prfB) constitute an operon that is transcribed from a vegetative (ςA-dependent) promoter located upstream ofsecA. Furthermore, using different independent methods, we found that secA expression occurred mainly in the exponential growth phase, reaching a maximal value almost precisely at the transition from exponential growth to the stationary growth phase. Following to this maximum, the de novo transcription ofsecA sharply decreased to a low basal level. Since at the time of maximal secA transcription the secretion activity of B. subtilis strongly increases, our results clearly demonstrate that the expression of at least one of the central components of the B. subtilis protein export apparatus is adapted to the increased demand for protein secretion. Possible mechanistic consequences are discussed.

scholarly journals Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine

2008 ◽  
Vol 74 (15) ◽  
pp. 4847-4852 ◽  
Author(s):  
Anastasia Matthies ◽  
Thomas Clavel ◽  
Michael Gütschow ◽  
Wolfram Engst ◽  
Dirk Haller ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Enzyme Induction ◽  
Growth Phase ◽  
Anaerobic Bacterium ◽  
Stationary Growth Phase ◽  
Gut Bacteria ◽  
Soy Isoflavones ◽  
Strictly Anaerobic ◽  
Stationary Growth ◽  
Mouse Intestine

ABSTRACT The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the Coriobacteriaceae. Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.

scholarly journals The extracellular proteome of Rhizobium etli CE3 in exponential and stationary growth phase

2010 ◽  
Vol 8 (1) ◽  
pp. 51 ◽  
Author(s):  
Niurka Meneses ◽  
Guillermo Mendoza-Hernández ◽  
Sergio Encarnación
Keyword(s):  
Growth Phase ◽  
Stationary Growth Phase ◽  
Rhizobium Etli ◽  
Stationary Growth ◽  
Extracellular Proteome

scholarly journals Proteomic Study of the Exponential-Stationary Growth Phase Transition in the Haloarchaea Natrialba magadii and Haloferax volcanii

PROTEOMICS ◽  
2018 ◽  
Vol 18 (14) ◽  
pp. 1800116 ◽  
Author(s):  
Micaela Cerletti ◽  
María Ines Giménez ◽  
Christian Tröetschel ◽  
Celeste D’ Alessandro ◽  
Ansgar Poetsch ◽  
...  
Keyword(s):  
Phase Transition ◽  
Growth Phase ◽  
Stationary Growth Phase ◽  
Haloferax Volcanii ◽  
Stationary Growth ◽  
Proteomic Study ◽  
Natrialba Magadii ◽  
Growth Phase Transition

Effect of Temperature on Growth and Alpha Toxin Production by Clostridium perfringens1

1979 ◽  
Vol 42 (11) ◽  
pp. 848-851 ◽  
Author(s):  
Y. PARK ◽  
E. M. MIKOLAJCIK
Keyword(s):  
Growth Phase ◽  
Incubation Temperature ◽  
Ground Beef ◽  
Population Level ◽  
Toxin Production ◽  
Stationary Growth Phase ◽  
Effect Of Temperature ◽  
Alpha Toxin ◽  
Stationary Growth ◽  
Incubation Temperatures

Growth and alpha toxin production by a strain of Clostridium perfringens was determined in Thioglycollate medium, beef broth with ground beef, and beef broth with ground beef and soy protein. Incubation temperatures ranged from 15 to 50 C. In Thioglycollate medium, maximum alpha toxin production occurred at 35 C and was 40 times greater than that observed at 45 C. However, generation time and maximum population were approximately the same at 35 and 45 C. At 15 C, a two log cycle reduction in viable counts occurred within 6 h. Irrespective of incubation temperature, alpha toxin levels in Thioglycollate medium declined as the incubation period was extended beyond the stationary growth phase. In the beef broth with ground beef system which was studied at 35 C only, the organism grew slower and produced less toxin than in Thioglycollate medium. The amount of alpha toxin detected was influenced to a greater extent by the incubation time and temperature, the holding time beyond the stationary growth phase, and the growth medium than by the population level of C. perfringens.

scholarly journals Various checkpoints prevent the synthesis ofStaphylococcus aureuspeptidoglycan hydrolase LytM in the stationary growth phase

RNA Biology ◽  
2016 ◽  
Vol 13 (4) ◽  
pp. 427-440 ◽  
Author(s):  
Efthimia Lioliou ◽  
Pierre Fechter ◽  
Isabelle Caldelari ◽  
Brian C. Jester ◽  
Sarah Dubrac ◽  
...  
Keyword(s):  
Growth Phase ◽  
Stationary Growth Phase ◽  
Stationary Growth

scholarly journals Poly-β-Hydroxybutyrate Production by the Cyanobacterium Scytonema geitleri Bharadwaja under Varying Environmental Conditions

Biomolecules ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 198 ◽  
Author(s):  
Manoj K. Singh ◽  
Pradeep K. Rai ◽  
Anuradha Rai ◽  
Surendra Singh ◽  
Jay Shankar Singh
Keyword(s):  
Carbon Source ◽  
Growth Phase ◽  
Environmental Conditions ◽  
Carbon Sources ◽  
Stationary Growth Phase ◽  
Stationary Growth ◽  
Cell Weight ◽  
Dry Cell Weight ◽  
Phb Production ◽  
Dry Cell

The production of poly-β-hydroxybutyrate (PHB) under varying environmental conditions (pH, temperature and carbon sources) was examined in the cyanobacterium Scytonema geitleri Bharadwaja isolated from the roof-top of a building. The S. geitleri produced PHB and the production of PHB was linear with the growth of cyanobacterium. The maximum PHB production (7.12% of dry cell weight) was recorded when the cells of S. geitleri were at their stationary growth phase. The production of PHB was optimum at pH 8.5 and 30 °C, and acetate (30 mM) was the preferred carbon source.

Sign in / Sign up

Export Citation Format

Share Document