Promotion of recombinant macrophage colony stimulating factor production by dimethyl sulfoxide addition in Chinese hamster ovary cells

2007 ◽  
Vol 103 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Chi-Hsien Liu ◽  
Li-Hsin Chen
Keyword(s):  
Dimethyl Sulfoxide ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Colony Stimulating Factor ◽  
Ovary Cells ◽  
Factor Production ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

scholarly journals Crystal structure of the Fab region of a neutralizing antibody against granulocyte-macrophage colony-stimulating factor

2019 ◽  
Vol 75 (10) ◽  
pp. 634-639
Author(s):  
Clement Angkawidjaja ◽  
Takashi Torashima
Keyword(s):  
Crystal Structure ◽  
Mononuclear Cells ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Human Antibody ◽  
Colony Stimulating Factor ◽  
Loop Region ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

An increased level of granulocyte-macrophage colony-stimulating factor has a potential role in the development of autoimmune diseases, and the neutralization of its activity by monoclonal antibodies is a promising therapy for some diseases. Here, the crystal structure of the Fab region of EV1007, a fully human antibody expressed in Chinese hamster ovary cells that was developed from human peripheral blood mononuclear cells, is described. The structure closely resembles that of MB007, which is the Fab region of the same antibody expressed in Escherichia coli [Blech et al. (2012), Biochem. J. 447, 205–215], except at the hinge regions between the immunoglobulin domains and the H3 loop region. This paper presents evidence for the flexibility of the hinge and H3 loop regions of the antibody based on the comparison of two independently solved crystal structures.

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