Design of carrier tRNAs and selection of four-base codons for efficient incorporation of various nonnatural amino acids into proteins in Spodoptera frugiperda 21 (Sf21) insect cell-free translation system

2006 ◽  
Vol 102 (6) ◽  
pp. 511-517 ◽  
Author(s):  
Masumi Taki ◽  
Yasunori Tokuda ◽  
Takashi Ohtsuki ◽  
Masahiko Sisido
Keyword(s):  
Amino Acids ◽  
Spodoptera Frugiperda ◽  
Insect Cell ◽  
Translation System ◽  
Nonnatural Amino Acids ◽  
Free Translation ◽  
Selection Of

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of

scholarly journals Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis

2010 ◽  
Vol 6 ◽  
Author(s):  
Shijie Ye ◽  
Allison Ann Berger ◽  
Dominique Petzold ◽  
Oliver Reimann ◽  
Benjamin Matt ◽  
...  
Keyword(s):  
Amino Acids ◽  
Translation System ◽  
Biologically Relevant ◽  
Fluorinated Amino Acids ◽  
Free Translation ◽  
Trna Species ◽  
A Cell ◽  
Protein Environment ◽  
Vitro Protein

This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of discrete fluorines’ interaction with canonical amino acids in a native protein environment and to enable the design of fluorinated proteins with arbitrary desired properties.

Incorporation of Unnatural Non-α-Amino Acids into the N Terminus of Proteins in a Cell-Free Translation System

ChemBioChem ◽  
2007 ◽  
Vol 8 (14) ◽  
pp. 1650-1653 ◽  
Author(s):  
Norihito Muranaka ◽  
Masanori Miura ◽  
Hikaru Taira ◽  
Takahiro Hohsaka
Keyword(s):  
Amino Acids ◽  
Translation System ◽  
N Terminus ◽  
Free Translation ◽  
A Cell

scholarly journals A Concept for Selection of Codon-Suppressor tRNAs Based on Read-Through Ribosome Display in anIn VitroCompartmentalized Cell-Free Translation System

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Atsushi Ogawa ◽  
Masayoshi Hayami ◽  
Shinsuke Sando ◽  
Yasuhiro Aoyama
Keyword(s):  
Stop Codon ◽  
Full Length ◽  
Translation System ◽  
Anticodon Loop ◽  
Ribosome Display ◽  
Amber Stop Codon ◽  
Free Translation ◽  
Translatable Mrna ◽  
Selection Of

Here is presented a concept forin vitroselection of suppressor tRNAs. It uses a pool of dsDNA templates in compartmentalized water-in-oil micelles. The template contains a transcription/translation trigger, an amber stop codon, and another transcription trigger for the anticodon- or anticodon loop-randomized gene for tRNASer. Upon transcription are generated two types of RNAs, a tRNA and a translatable mRNA (mRNA-tRNA). When the tRNA suppresses the stop codon (UAG) of the mRNA, the full-length protein obtained upon translation remains attached to the mRNA (read-through ribosome display) that contains the sequence of the tRNA. In this way, the active suppressor tRNAs can be selected (amplified) and their sequences read out. The enriched anticodon (CUA) was complementary to the UAG stop codon and the enriched anticodon-loop was the same as that in the natural tRNASer.

Incorporation of Nonnatural Amino Acids into Proteins by Using Various Four-Base Codons in anEscherichia coliin Vitro Translation System

Biochemistry ◽  
2001 ◽  
Vol 40 (37) ◽  
pp. 11060-11064 ◽  
Author(s):  
Takahiro Hohsaka ◽  
Yuki Ashizuka ◽  
Hikaru Taira ◽  
Hiroshi Murakami ◽  
Masahiko Sisido
Keyword(s):  
Amino Acids ◽  
Translation System ◽  
Nonnatural Amino Acids
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