A knockout strain of CPR1 induced during fermentation of Saccharomyces cerevisiae KNU5377 is susceptible to various types of stress

2006 ◽  
Vol 102 (4) ◽  
pp. 288-296 ◽  
Author(s):  
Il-Sup Kim ◽  
Hae-Sun Yun ◽  
In-Su Park ◽  
Ho-Yong Sohn ◽  
Hitoshi Iwahashi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Knockout Strain ◽  
Saccharomyces Cerevisiae Knu5377

scholarly journals TMOD-38. PRODUCTION OF D-2-HYDROXYGLUTARATE IN SACCHAROMYCES CEREVISIAE LEADS TO GROWTH IMPAIRMENT AND DECREASED SURVIVAL

2019 ◽  
Vol 21 (Supplement_6) ◽  
pp. vi271-vi271
Author(s):  
Sophie Fiola ◽  
Eli Ganni ◽  
Rita Lo ◽  
Ka Yee Lok ◽  
Elena Kuzmin ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Gene Interactions ◽  
Low Grade ◽  
Yeast Saccharomyces Cerevisiae ◽  
Growth Impairment ◽  
Knockout Strain ◽  
Genomic Array ◽  
Negative Gene

Abstract High levels of D-2-hydroxyglutarate (D2HG) are found in several types of cancers, most notably low grade gliomas (LGGs). The accumulation of D-2HG contributes to tumorigenesis through a variety of mechanisms including decreased utilization of oxidative phosphorylation and histone hypermethylation. The use of the budding yeast Saccharomyces cerevisiae as a model system to study cancer allows for faster, more efficient elucidation of various molecular mechanisms, including functional genomics via genomic array screening. S. cerevisiae encodes two homologs of the human D-2HG dehydrogenase: the mitochondrial Dld2 and cytosolic Dld3. We detected an increase in the production of D-2HG in the dld3∆ knockout strain by LC-MS. In addition, the dld3∆ knockout strain shows decreased survival and a growth impairment in glucose-containing liquid media. However, this strain did not show a significant growth impairment on glucose or glycerol-containing solid media. Using publicly available Synthetic Genomic Array (SGA) analysis data from TheCellMap.org, we investigated the top negative gene interactions for our dld3 knockout strain. GO analysis of these negative gene interactions showed enrichment of targets locating to the mitochondria, suggesting that the increase of 2-HG leads to mitochondrial impairment, consistent with previous observations in other models of LGGs. The top two targets of the SGA screen were mdm35, a mitochondrial interspace membrane protein involved in assembly of the mitochondrial respiratory chain complex and cdc8, a component of the de novo pyrimidine biosynthesis pathway. Taken together, these results suggest that the dld3∆ knockout strain is an appropriate model in which to study the D-2HG-driven changes that occur during tumorigenesis.

scholarly journals Saccharomyces cerevisiae KNU5377 stress response during high-temperature ethanol fermentation

2013 ◽  
Vol 35 (3) ◽  
pp. 210-218 ◽  
Author(s):  
Il-Sup Kim ◽  
Young-Saeng Kim ◽  
Hyun Kim ◽  
Ingnyol Jin ◽  
Ho-Sung Yoon
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Temperature ◽  
Stress Response ◽  
Ethanol Fermentation ◽  
Saccharomyces Cerevisiae Knu5377

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel
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