Removal of hydrogen sulfide by sulfate-resistant Acidithiobacillus thiooxidans AZ11

2006 ◽  
Vol 101 (4) ◽  
pp. 309-314 ◽  
Author(s):  
Eun Young Lee ◽  
Nae Yoon Lee ◽  
Kyung-Suk Cho ◽  
Hee Wook Ryu
Keyword(s):  
Hydrogen Sulfide ◽  
Acidithiobacillus Thiooxidans

scholarly journals Removal of sulfur contaminants from biogas to enable direct catalytic methanation

2019 ◽  
Author(s):  
Christian Dannesboe ◽  
John Bøgild Hansen ◽  
Ib Johannsen
Keyword(s):  
Hydrogen Sulfide ◽  
Dimethyl Sulfide ◽  
Renewable Energy Sources ◽  
Sulfur Removal ◽  
Carbonyl Sulfide ◽  
Acidithiobacillus Thiooxidans ◽  
Carbon Utilization ◽  
Combined System ◽  
Renewable Biomass ◽  
Biogas Reactor

AbstractIn the near future, renewable energy sources will replace fossil energy. To allow full carbon utilization of renewable biomass, we have demonstrated a possible integration between a biogas reactor, an electrolysis unit, and a catalytic methanation reactor. Stringent removal of all sulfur contaminants in raw biogas is required to enable this integration. We demonstrate how existing bulk sulfur removal solutions, like a biotrickling filter loaded with Acidithiobacillus thiooxidans and impregnated activated carbon, are unable to meet this requirement. Only the main sulfur contaminant hydrogen sulfide (H2S) can effectively be removed. Contaminants carbon disulfide (CS2), dimethyl sulfide (DMS), and carbonyl sulfide (COS) will leak through the carbon filter, long before hydrogen sulfide can be detected. Utilization of surplus oxygen from the combined system is proven problem free and allows sulfur removal without introducing contaminants. Provided that a recommended sulfur guard is included, the proposed design is ready for full-scale implementation.

scholarly journals An Optical Microbial Biosensor Based on Whole Cell of Acidithiobacillus thiooxidans for Hydrogen Sulfide Determination

2018 ◽  
Vol 6 (1) ◽  
pp. 42
Author(s):  
Odemar Cardoso Silva ◽  
Andréa Medeiros Salgado ◽  
Francisca Pessoa De França
Keyword(s):  
Hydrogen Sulfide ◽  
Elemental Sulfur ◽  
Low Cost ◽  
Acidithiobacillus Thiooxidans ◽  
Bacterial Cells ◽  
Low Oxygen ◽  
Microbial Biosensor ◽  
Biosensor Application ◽  
Good Repeatability ◽  
Low Oxygen Concentration

Due to its corrosive and highly toxic character, the generation of hydrogen sulfide is a serious problem for the environment, human health, and the industry. This paper reported a new and simple methodology for aqueous hydrogen sulfide determination through the development of an optical microbial biosensor. The principle of detection was based on the aerobic and chemolithotrophic metabolism of Acidithiobacillus thiooxidans bacterial cells. Under low oxygen concentration and acidic conditions A. thiooxidans can rapidly oxidize H2S to elemental sulfur. The biochemical formation of elemental sulfur can be spectrophotometrically detected and the increase in absorbance at 620 nm exhibited a linear relationship to an H2S concentration up to 100 mg.L-1. The parameters concerning the analytical performance of the biosensor such as cell harvesting time and pH influence were measured and optimized through the optical absorption value. The biosensor was selective to H2S with no important disturbance by tested species except thiosulfate ion (11.5% error). Biosensor response expressed good repeatability (RSD = 4.46 %) and reproducibility (RSD = 4.66 %). The low cost of cell cultivation and the absence of the immobilization step make feasible the optic biosensor application.

Hydrogen sulfide removal from air by Acidithiobacillus thiooxidans in a trickle bed reactor

2009 ◽  
Vol 54 (5) ◽  
pp. 409-414 ◽  
Author(s):  
M. Ramirez ◽  
J. M. Gómez ◽  
D. Cantero ◽  
J. Páca ◽  
M. Halecký ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Acidithiobacillus Thiooxidans ◽  
Trickle Bed Reactor ◽  
Sulfide Removal ◽  
Hydrogen Sulfide Removal ◽  
Trickle Bed

Endogenous hydrogen sulfide sulfhydrates IKKβ at cysteine 179 to control pulmonary artery endothelial cell inflammation

2019 ◽  
Vol 133 (20) ◽  
pp. 2045-2059 ◽  
Author(s):  
Da Zhang ◽  
Xiuli Wang ◽  
Siyao Chen ◽  
Selena Chen ◽  
Wen Yu ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Endothelial Cell ◽  
Pulmonary Artery ◽  
Cell Model ◽  
Site Directed Mutagenesis ◽  
Critical Event ◽  
Hypertensive Rats ◽  
Pulmonary Artery Endothelial Cell

Abstract Background: Pulmonary artery endothelial cell (PAEC) inflammation is a critical event in the development of pulmonary arterial hypertension (PAH). However, the pathogenesis of PAEC inflammation remains unclear. Methods: Purified recombinant human inhibitor of κB kinase subunit β (IKKβ) protein, human PAECs and monocrotaline-induced pulmonary hypertensive rats were employed in the study. Site-directed mutagenesis, gene knockdown or overexpression were conducted to manipulate the expression or activity of a target protein. Results: We showed that hydrogen sulfide (H2S) inhibited IKKβ activation in the cell model of human PAEC inflammation induced by monocrotaline pyrrole-stimulation or knockdown of cystathionine γ-lyase (CSE), an H2S generating enzyme. Mechanistically, H2S was proved to inhibit IKKβ activity directly via sulfhydrating IKKβ at cysteinyl residue 179 (C179) in purified recombinant IKKβ protein in vitro, whereas thiol reductant dithiothreitol (DTT) reversed H2S-induced IKKβ inactivation. Furthermore, to demonstrate the significance of IKKβ sulfhydration by H2S in the development of PAEC inflammation, we mutated C179 to serine (C179S) in IKKβ. In purified IKKβ protein, C179S mutation of IKKβ abolished H2S-induced IKKβ sulfhydration and the subsequent IKKβ inactivation. In human PAECs, C179S mutation of IKKβ blocked H2S-inhibited IKKβ activation and PAEC inflammatory response. In pulmonary hypertensive rats, C179S mutation of IKKβ abolished the inhibitory effect of H2S on IKKβ activation and pulmonary vascular inflammation and remodeling. Conclusion: Collectively, our in vivo and in vitro findings demonstrated, for the first time, that endogenous H2S directly inactivated IKKβ via sulfhydrating IKKβ at Cys179 to inhibit nuclear factor-κB (NF-κB) pathway activation and thereby control PAEC inflammation in PAH.

Limnological characteristics, community metabolism and management strategies of a coastal sinkhole in Cuba (Cenote Jennifer)

2020 ◽  
Vol 56 ◽  
pp. 24
Author(s):  
Roberto González-De Zayas ◽  
Liosban Lantigua Ponce de León ◽  
Liezel Guerra Rodríguez ◽  
Felipe Matos Pupo ◽  
Leslie Hernández-Fernández
Keyword(s):  
Hydrogen Sulfide ◽  
Primary Productivity ◽  
Major Ions ◽  
Saline Water ◽  
Management Strategies ◽  
Tourist Destination ◽  
Community Metabolism ◽  
Morphological Studies ◽  
Made In ◽  
Limnological Parameters

The Cenote Jennifer is an important and unique aquatic sinkhole in Cayo Coco (Jardines del Rey Tourist Destination) that has brackish to saline water. Two samplings were made in 1998 and 2009, and 4 metabolism community experiments in 2009. Some limnological parameters were measured in both samplings (temperature, salinity, pH, dissolved oxygen major ions, hydrogen sulfide, nutrients and others). Community metabolism was measured through incubated oxygen concentration in clear and dark oxygen bottles. Results showed that the sinkhole limnology depends on rainfall and light incidence year, with some stratification episodes, due to halocline or oxycline presence, rather than thermocline. The sinkhole water was oligotrophic (total nitrogen of 41.5 ± 22.2 μmol l−1 and total phosphorus of 0.3 ± 0.2 μmol l−1) and with low productivity (gross primary productivity of 63.0 mg C m−2 d−1). Anoxia and hypoxia were present at the bottom with higher levels of hydrogen sulfide, lower pH and restricted influence of the adjacent sea (2 km away). To protect the Cenote Jennifer, tourist exploitation should be avoided and more resources to ecological and morphological studies should be allocated, and eventually use this aquatic system only for specialized diving. For conservation purposes, illegal garbage disposal in the surrounding forest should end.

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