Gene expression property of high-density three-dimensional tissue of HepG2 cells formed in radial-flow bioreactor

2006 ◽  
Vol 101 (3) ◽  
pp. 243-250 ◽  
Author(s):  
Tomokatsu Hongo ◽  
Mariko Kajikawa ◽  
Seiichi Ishida ◽  
Shogo Ozawa ◽  
Yasuo Ohno ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hepg2 Cells ◽  
Radial Flow ◽  
Three Dimensional ◽  
High Density ◽  
Radial Flow Bioreactor

Three-dimensional high-density culture of HepG2 cells in a 5-ml radial-flow bioreactor for construction of artificial liver

2005 ◽  
Vol 99 (3) ◽  
pp. 237-244 ◽  
Author(s):  
Tomokatsu Hongo ◽  
Mariko Kajikawa ◽  
Seiichi Ishida ◽  
Shogo Ozawa ◽  
Yasuo Ohno ◽  
...  
Keyword(s):  
Hepg2 Cells ◽  
Radial Flow ◽  
Three Dimensional ◽  
High Density ◽  
Artificial Liver ◽  
High Density Culture ◽  
Radial Flow Bioreactor

scholarly journals Production and release of infectious hepatitis C virus from human liver cell cultures in the three-dimensional radial-flow bioreactor

Virology ◽  
2003 ◽  
Vol 314 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Hideki Aizaki ◽  
Seishi Nagamori ◽  
Mami Matsuda ◽  
Hayato Kawakami ◽  
Osamu Hashimoto ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Cultures ◽  
Liver Cell ◽  
Human Liver ◽  
Radial Flow ◽  
Three Dimensional ◽  
Infectious Hepatitis ◽  
Human Liver Cell ◽  
Radial Flow Bioreactor

Reconstruction of Liver Organoid Using an Apatite-Fiber Scaffold, a Radial-Flow Bioreactor and FCL-4 Cells of Hepatocyte Model

2007 ◽  
Vol 361-363 ◽  
pp. 1165-1168 ◽  
Author(s):  
Mamoru Aizawa ◽  
A. Hiramoto ◽  
H. Maehashi ◽  
Tomokazu Matsuura
Keyword(s):  
Tissue Engineering ◽  
Single Crystal ◽  
Bone Tissue Engineering ◽  
Radial Flow ◽  
Three Dimensional ◽  
3D Culture ◽  
High Porosity ◽  
Fiber Scaffolds ◽  
Radial Flow Bioreactor ◽  
Culture Of Hepatocytes

We have previously developed apatite-fiber scaffolds (AFSs) for bone tissue engineering using single-crystal apatite fibers and carbon beads. In the present investigation, we examined the possibility of reconstruction of a liver organoid via three-dimensional (3D) culture of hepatocytes using the AFSs and the radial-flow bioreactor (RFB), aiming to apply the scaffold as a matrix for regeneration of a real organ. FLC-4 cells were used as a model of hepatocyte. The cells were well-viable in the RFB settled with AFSs over a period for 28 d, compared with the cases of cellulose beads and apatite beads with high porosity of 85%. We conclude that the present AFS may be a promising scaffold for tissue engineering of liver.

scholarly journals Influence of Culture Period on Osteoblast Differentiation of Tissue-Engineered Bone Constructed by Apatite-Fiber Scaffolds Using Radial-Flow Bioreactor

2021 ◽  
Vol 22 (23) ◽  
pp. 13080
Author(s):  
Kitaru Suzuki ◽  
Jun Fukasawa ◽  
Maiko Miura ◽  
Poon Nian Lim ◽  
Michiyo Honda ◽  
...  
Keyword(s):  
Osteoblast Differentiation ◽  
Radial Flow ◽  
Bone Marrow Cells ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Bone Diseases ◽  
Culture Period ◽  
Alizarin Red ◽  
Fiber Scaffolds ◽  
Radial Flow Bioreactor

With the limitation of autografts, the development of alternative treatments for bone diseases to alleviate autograft-related complications is highly demanded. In this study, a tissue-engineered bone was formed by culturing rat bone marrow cells (RBMCs) onto porous apatite-fiber scaffolds (AFSs) with three-dimensional (3D) interconnected pores using a radial-flow bioreactor (RFB). Using the optimized flow rate, the effect of different culturing periods on the development of tissue-engineered bone was investigated. The 3D cell culture using RFB was performed for 0, 1 or 2 weeks in a standard medium followed by 0, 1 or 2 weeks in a differentiation medium. Osteoblast differentiation in the tissue-engineered bone was examined by alkaline phosphatase (ALP) and osteocalcin (OC) assays. Furthermore, the tissue-engineered bone was histologically examined by hematoxylin and eosin and alizarin red S stains. We found that the ALP activity and OC content of calcified cells tended to increase with the culture period, and the differentiation of tissue-engineered bone could be controlled by varying the culture period. In addition, the employment of RFB and AFSs provided a favorable 3D environment for cell growth and differentiation. Overall, these results provide valuable insights into the design of tissue-engineered bone for clinical applications.

Dynamic behavior of hepatitis C virus quasispecies in a long-term culture of the three-dimensional radial-flow bioreactor system

2008 ◽  
Vol 148 (1-2) ◽  
pp. 174-181 ◽  
Author(s):  
Kyoko Murakami ◽  
Yasushi Inoue ◽  
Su-Su Hmwe ◽  
Kazuhiko Omata ◽  
Tomokatsu Hongo ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Dynamic Behavior ◽  
Radial Flow ◽  
Three Dimensional ◽  
Long Term Culture ◽  
Bioreactor System ◽  
Radial Flow Bioreactor
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