scholarly journals Effects of Fatty Acid-Free Bovine Serum Albumin and Fetal Calf Serum Supplementing Repair Cultures on Pre- and Post-Warm Viability of Biopsied Bovine Embryos Produced In Vitro

2004 ◽  
Vol 50 (4) ◽  
pp. 471-476 ◽  
Author(s):  
Kenji YOTSUSHIMA ◽  
Minoru SAKAGUCHI ◽  
Manabu SHIMIZU ◽  
Tomoko OKIMURA ◽  
Yoshiaki IZAIKE
Keyword(s):  
Fatty Acid ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Bovine Embryos

survival of bovine embryos frozen in media supplemented with newborn calf serum or bovine serum albumin

1984 ◽  
Vol 21 (1) ◽  
pp. 223 ◽  
Author(s):  
K.R. Bondioli ◽  
C.B. Brunson ◽  
C.R. Looney ◽  
J.M. Massey ◽  
A.B. McGrath ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Calf Serum ◽  
Bovine Embryos ◽  
Newborn Calf Serum

Oxygen concentration and protein source affect the development of preimplantation goat embryos in vitro

1991 ◽  
Vol 3 (5) ◽  
pp. 601 ◽  
Author(s):  
PA Batt ◽  
DK Gardner ◽  
AW Cameron
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Oxygen Concentration ◽  
Bovine Serum ◽  
Calf Serum ◽  
Cell Number ◽  
The Mean ◽  
Number Of Cells

The effect of oxygen concentration and the source of protein in culture medium on the development of 2- to 4-cell goat embryos in vitro was investigated. Embryos were collected from superovulated Angora-Cashmere-cross goats 48 h after ovulation and cultured for 6 days in synthetic oviduct fluid (SOF) medium under one of two oxygen concentrations (20% or 7%) and in the presence of one of five protein sources; Miles bovine serum albumin (Miles BSA), Commonwealth Serum Laboratory bovine serum albumin (CSL BSA), goat serum (GS), fetal calf serum (FCS) and human serum (HS). In the presence of 20% oxygen the percentage of embryos reaching the expanded and/or hatched blastocyst stage in SOF medium containing Miles BSA was 29%, with a mean cell number per embryo of 28.1 +/- 6.0 (+/- s.e.m.). Use of an oxygen concentration of 7% significantly increased the percentage of embryos reaching this stage (80%, P less than 0.01) and the mean number of cells per embryo (65.3 +/- 8.2, P less than 0.01). The mean number of cells of the early-cleavage-stage embryos was significantly lower when the medium contained CSL BSA, GS or FCS (42.7 +/- 5.6, 29.0 +/- 6.1 and 21.3 +/- 3.2, respectively) than with Miles BSA (92.8 +/- 6.4) or HS (104.8 +/- 17.2) (P less than 0.01). Under 7% oxygen and with Miles BSA or HS, embryos were morphologically comparable to those developed in vivo, but the mean cell numbers in vitro were only approximately half those obtained in vivo.

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