scholarly journals Embryonic Fibroblast-Conditioned Medium Enhances Viability and Proliferation of Chick Circulating Primordial Germ Cells (cPGCs) in Suspension Culture

2002 ◽  
Vol 48 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Takaharu KAWASHIMA ◽  
Koichiro KANO ◽  
Yasuyuki KANNAN ◽  
Yoshinori TAMAKI ◽  
Koichiro HASHIMOTO
Keyword(s):  
Suspension Culture ◽  
Conditioned Medium ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Embryonic Fibroblast

TGF beta 1 inhibits proliferation and has a chemotropic effect on mouse primordial germ cells in culture

Development ◽  
1991 ◽  
Vol 113 (4) ◽  
pp. 1451-1457 ◽  
Author(s):  
I. Godin ◽  
C.C. Wylie
Keyword(s):  
Germ Cells ◽  
Culture System ◽  
Primordial Germ Cells ◽  
Target Tissue ◽  
Tgf Beta ◽  
Soluble Factors ◽  
Related Molecule ◽  
Feeder Layers ◽  
Stem Cell Populations ◽  
Embryonic Fibroblast

Primordial germ cells are the stem cells that provide the functional gametes of adult animals. In many animal groups they are set aside at the earliest stages of development, and migrate from their sites of first appearance to the sites where the gonad will form, the genital ridges. During this migration they proliferate. In the mouse embryo their numbers increase from less than one hundred to approximately four thousand during the period of their migration. In a previous paper we showed that both the proliferation and the direction of migration of mouse PGCs in culture were influenced by soluble factors released from their target tissue, the genital ridges. Studies on other stem cell populations have shown that complex combinations of growth factors control their proliferation, migration and differentiation. In this paper, we show that TGF beta 1 inhibits proliferation of PGCs taken from 8.5 day old embryos and cultured on embryonic fibroblast feeder layers. We also show that the previously reported chemotropic effect of genital ridges in this culture system is mediated by TGF beta 1, or a closely related molecule, released from the genital ridges.

Novel growth factor supporting survival of murine primordial germ cells: evidence from conditioned medium ofter fetal gonadal somatic cells

2001 ◽  
Vol 60 (3) ◽  
pp. 384-396 ◽  
Author(s):  
Shuji Takabayashi ◽  
Yumiko Sasaoka ◽  
Masayo Yamashita ◽  
Toshinobu Tokumoto ◽  
Katsutoshi Ishikawa ◽  
...  
Keyword(s):  
Growth Factor ◽  
Conditioned Medium ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Somatic Cells

Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

1992 ◽  
Vol 50 (1) ◽  
pp. 668-669
Author(s):  
Amreek Singh ◽  
Warren G. Foster ◽  
Anna Dykeman ◽  
David C. Villeneuve
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Surface Epithelium ◽  
Morphological Parameters ◽  
Comprehensive Investigation ◽  
Ovary Surface Epithelium ◽  
Rat Ovary ◽  
High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

Transfusion of Chick Primordial Germ Cells into Quail Embryos and their Settlement in Gonads

1998 ◽  
Vol 69 (10) ◽  
pp. 911-915 ◽  
Author(s):  
Tamao ONO ◽  
Ryohei YOKOI ◽  
Seishi MAEDA ◽  
Takao NISHIDA ◽  
Hirohiko AOYAMA
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells
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