scholarly journals Parthenogenetic Activation Induced by Progesterone in Cultured Mouse Oocytes.

1995 ◽  
Vol 41 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Hiroshi IMAHIE ◽  
Eimei SATO ◽  
Yutaka TOYODA
Keyword(s):  
Parthenogenetic Activation ◽  
Mouse Oocytes

Parthenogenetic activation of mouse oocytes by 6-dimethylaminopurine

1997 ◽  
Vol 47 (1) ◽  
pp. 208 ◽  
Author(s):  
Honglin Liu ◽  
Biqin Fan ◽  
Hehai Wang
Keyword(s):  
Parthenogenetic Activation ◽  
Mouse Oocytes

Cell cycle progression of parthenogenetically activated mouse oocytes to interphase is dependent on the level of internal calcium

1992 ◽  
Vol 103 (2) ◽  
pp. 389-396 ◽  
Author(s):  
C. Vincent ◽  
T.R. Cheek ◽  
M.H. Johnson
Keyword(s):  
Cell Cycle Progression ◽  
Polar Body ◽  
Calcium Ionophore ◽  
Cytosolic Calcium ◽  
Calcium Ionophore A23187 ◽  
Mouse Oocyte ◽  
Free Calcium ◽  
Ionophore A23187 ◽  
Parthenogenetic Activation ◽  
Mouse Oocytes

Nuclear maturation of the mouse oocyte becomes arrested in metaphase of the second meiotic division (MII). Fertilization or parthenogenetic activation induces meiotic completion, chromosomal decondensation and formation of a pronucleus. This completion of meiosis is probably triggered by a transient increase in cytosolic calcium ions. When activated just after ovulation by a low concentration of the calcium ionophore A23187, the majority of the mouse oocytes go through a metaphase to anaphase transition and extrude their second polar body but they do not proceed into interphase; instead their chromatids remain condensed and a microtubular metaphase spindle reforms (metaphase III). However, a high percentage of these oocytes will undergo a true parthenogenetic activation assessed by the formation of a pronucleus, when exposed to a higher concentration of the calcium ionophore. The capacity of the mouse oocyte to pass into metaphase III is lost with increasing time post-ovulation. Direct measurement of intracellular calcium with Fura-2 reveals higher levels of cytosolic calcium in aged oocytes and/or using higher concentrations of calcium ionophore for activation. It is concluded that the internal free calcium level determines the transition to interphase.

Parthenogenetic activation of mouse oocytes following avertin anaesthesia

Development ◽  
1975 ◽  
Vol 33 (4) ◽  
pp. 941-946
Author(s):  
M. H. Kaufman
Keyword(s):  
Simple Method ◽  
Parthenogenetic Activation ◽  
Mouse Oocytes ◽  
Parthenogenetic Development ◽  
Ovulatory Period ◽  
Mouse Eggs ◽  
Post Implantation

Avertin anaesthesia induced mouse eggs to become activated parthenogenetically. An increasing incidence of activation was observed when females were anaesthetized 6·5, 9 and 13 h after ovulation, reaching a maximum of 45·8 %. In a spontaneously ovulating group approximately 12·5 % of all the eggs ovulated, or 27·3 % of all the eggs activated evoked a decidual response, and the presence of implanting embryos was confirmed histologically. These findings demonstrate a new and simple method of inducing post-implantation parthenogenetic development in the mouse, and stress the necessity of taking into account the possible consequences of anaesthesia in the early post-ovulatory period.

Comparison between different combinations of chemical treatment on parthenogenetic activation of mouse oocytes and its subsequent embryonic development

2013 ◽  
Vol 17 (3) ◽  
pp. 196-202 ◽  
Author(s):  
Siti Khadijah Idris ◽  
Ramli Bin Abdullah ◽  
Wan Khadijah Wan Embong ◽  
Mohammad Mijanur Rahman
Keyword(s):  
Embryonic Development ◽  
Chemical Treatment ◽  
Parthenogenetic Activation ◽  
Mouse Oocytes

The effect of propofol on parthenogenetic activation, in vitro fertilization and early development of mouse oocytes

1997 ◽  
Vol 67 (4) ◽  
pp. 769-774 ◽  
Author(s):  
Cécile Janssenswillen ◽  
Frank Christiaens ◽  
Frédéric Camu ◽  
André Van Steirteghem
Keyword(s):  
In Vitro Fertilization ◽  
Early Development ◽  
Vitro Fertilization ◽  
Parthenogenetic Activation ◽  
Mouse Oocytes
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