scholarly journals Quantitative Analysis of Coat-color Patterns in BALB/c .DARRLR. C57BL/6 Mouse Chimeras by Means of a Computer-Based Color Image Processor System.

1995 ◽  
Vol 41 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Sang-Yong KIM ◽  
Yasunari KAWASHIMA ◽  
Jutaro TAKAHASHI ◽  
Yasuhisa YASUDA
Keyword(s):  
Quantitative Analysis ◽  
Coat Color ◽  
Color Image ◽  
Color Patterns ◽  
Image Processor ◽  
Mouse Chimeras ◽  
Computer Based

scholarly journals Novel insights into Sabino1 and splashed white coat color patterns in horses

2018 ◽  
Vol 49 (3) ◽  
pp. 249-253 ◽  
Author(s):  
T. Druml ◽  
G. Grilz-Seger ◽  
M. Neuditschko ◽  
M. Horna ◽  
A. Ricard ◽  
...  
Keyword(s):  
Coat Color ◽  
White Coat ◽  
Color Patterns

scholarly journals Effects of the white allele of the mi locus on coat pigmentation in chimeric mice

1994 ◽  
Vol 63 (3) ◽  
pp. 175-181
Author(s):  
Boris V. Konyukhov ◽  
Boris N. Kindyakov ◽  
Natalia A. Malinina
Keyword(s):  
Proliferative Activity ◽  
Coat Color ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Electrophoretic Analysis ◽  
Hair Follicles ◽  
Pigment Cells ◽  
Color Patterns ◽  
Proliferation And Differentiation ◽  
Almost All

SummaryTo investigate the cellular action of the Miωh allele in the mouse with regard to its effects upon coat color patterns, we generated a series of aggregation chimeras, using embryos that differ in their mi locus genotype. We have obtained 11 chimeras Miωh/ + C/C↔ + / + c/c and 8 chimeras + / + C/C↔ + / + c/c. Chimerism was determined by coat and retinal pigment epithelium mosaicism and by the electrophoretic analysis of GPI-1 isoenzymes. In Miωh/+ C/C↔, +/+ c/c mice white coat color prevailed due to the higher percentage of unpigmented areas and the higher percentage of unpigmented hairs in pigmented areas. Our data indicate that a single Miωh gene dose decreases the melanoblast proliferative activity, causing the lightening of coat pigmentation. In Miωh/ + C/C↔+/+ c/c mice a few pigmented hairs were often detected on the belly where Miωh / + mice always had a white spot. This suggests that in the chimeras the presence of some non-Miωh cells in the skin of the belly allows pigment cells to develop. Using embryos of two substrains of Miωh/Miωh mice that differ in their Gpi-1 locus genotype we have produced 8 Miωh/ + ↔ Miωh/Miωh chimeras. In all these chimeras coat color patterns resembled those of Miωh/ + heterozygotes despite the higher percentage of the Miwh/Miωh component in three chimeras. Mosaic hairs were absent in the chimeras. This shows that the chimeras have only one Miωh/ + melanoblast population which actively proliferates and colonizes almost all hair follicles. Thus the Miωh/Miωh dermis and epidermis do not suppress proliferation and differentiation of the Miωh/ + melanoblasts except the certain area on the belly.

Computer-assisted Instruction in Forestry

1978 ◽  
Vol 76 (9) ◽  
pp. 570-573
Author(s):  
Dieter R. Pelz
Keyword(s):  
United States ◽  
Quantitative Analysis ◽  
Forest Management ◽  
Simulation Models ◽  
The United States ◽  
Computer Assisted Instruction ◽  
Computer Assisted ◽  
Recent Developments ◽  
Computer Based ◽  
Assisted Instruction

Abstract A 1976 survey of 58 schools in the United States and Canada showed that computer-assisted instruction is widely used, most frequently in forest management, silviculture, and quantitative analysis courses. A total of 92 simulation models were identified. Recent developments are demonstrated by PLATO, a computer-based education system.

scholarly journals Application of an image analyzer to gold labeling in immunoelectron microscopy to achieve better demonstration and quantitative analysis.

1993 ◽  
Vol 41 (1) ◽  
pp. 123-128 ◽  
Author(s):  
H Shimizu ◽  
T Nishikawa
Keyword(s):  
Quantitative Analysis ◽  
Electron Micrographs ◽  
Color Image ◽  
Ultrastructural Localization ◽  
Immunogold Electron Microscopy ◽  
Image Analyzer ◽  
Threshold Method ◽  
Gold Particles ◽  
Type Vii Collagen ◽  
Membrane Antigens

An image analyzer was applied to pre- and post-embedding immunogold labeling with 5-nm gold probes in electron micrographs of several skin basement membrane antigens to improve the visualization of immunolabeling. With a TV camera connected to a color image analyzer, an image of an original immunoelectron micrograph was projected on a TV screen. The image was recorded in the analyzer as Record 1. After the floating threshold method procedure to reduce the contrast of the skin structure, electron-dense 5-nm gold particles could be easily detected. With the analyzer, these particles were then suitably enhanced in color and in size and their image was recorded as Record 2. Records 1 and 2 were then overlapped on the TV screen to build up a double-image picture. Compared with the small, electron-dense 5-nm gold particles in the original electron micrograph, ultrastructural localization of bullous pemphigoid antigen, epidermolysis bullosa acquisita antigen, and the collagenous part of Type VII collagen were more clearly and easily seen, even on low-magnification electron micrographs. The level of background labeling could also be accurately and objectively evaluated. By counting the number of gold particles labeling a certain area and using the analyzer to interpret the result as a diagram, quantitative analysis was also possible. We believe that this technique should be widely applicable to immunogold electron microscopy, not only of skin but also of other substrates of interest.

Ultrastructural studies of pigmented, depigmented and melanomatous skin in lipizzaner horses.

1978 ◽  
Vol 36 (2) ◽  
pp. 654-655
Author(s):  
W. Gebhart ◽  
G. Niebauer
Keyword(s):  
Coat Color ◽  
Energy Dispersive Spectrometer ◽  
Surgical Excision ◽  
Tumor Formation ◽  
Ultrastructural Studies ◽  
Biopsy Specimens ◽  
Suitable Animal Model ◽  
Computer Based ◽  
Melanotic Tumors ◽  
Dark Color

Lipizzaner horses - as well as some other graying breeds - undergo progressive depigmentation of their coat color and are regularily affected by melanotic tumors at older age. Although deeply pigmented at birth nearly all individuals loose their dark color at the age of 4 — 6 years and multiple melanotic tumors arise in skin. Unique melanomatosis is frequently present in 20 — 30 years old animal (Gebhart and Niebauer, 1977). These characteristics design the horses of the genetically well-defined Lipizzaner breed as a suitable animal model for studies on depigmentation and tumor formation processes. Histochemical and ultrastructural investigations were performed in order to elucidate the morphological substrates of pigmented and depigmented lesions.Biopsy specimens were obtained by surgical excision from pigmented and depigmented skin and from melanomas of 19 Lipizzaner horses. Immediately after excision the tissue was devided into several portions and processed for routine histology, DOPA-reaction and electron microscopy. Ultrastructural investigations were performed in a ZEISS EM 9S2 and a JEOL lOO C TEMSCAN electron microscope fitted with a computer based energy-dispersive spectrometer (LINK).

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