scholarly journals Quantitative Analysis of the Expression of Whey Acidic Protein (WAP) mRNA in the Prolactin-Responsive Mouse Mammary Gland Epithelial Cell Line, HC11.

1995 ◽  
Vol 41 (4) ◽  
pp. 331-338 ◽  
Author(s):  
Yuji KAWAMATA ◽  
Junko ISHIJIMA ◽  
Satoshi TANAKA ◽  
Masato SASAKI ◽  
Jumpei ENAMI ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Quantitative Analysis ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mouse Mammary Gland ◽  
Whey Acidic Protein ◽  
Acidic Protein ◽  
Epithelial Cell Line ◽  
Mammary Gland Epithelial Cell

Cell-Cell Contact Down-Regulates Expression of Membrane Type MetaIIoproteinase-1 (MT1-MMP) in a Mouse Mammary Gland Epithelial Cell Line

1997 ◽  
Vol 14 (1) ◽  
pp. 95-99 ◽  
Author(s):  
Satomi S. Tanaka ◽  
Yukiyasu Mariko ◽  
Hidetoshi Mori ◽  
Junko Ishijima ◽  
Sumie Tachi ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mouse Mammary Gland ◽  
Epithelial Cell Line ◽  
Cell Contact ◽  
Mammary Gland Epithelial Cell ◽  
Membrane Type ◽  
Cell Cell

Demonstration of Type II Hemidesmosomes in a Mammary Gland Epithelial Cell Line, BMGE-H1

1994 ◽  
Vol 115 (3) ◽  
pp. 469-476 ◽  
Author(s):  
Jun Uematsu ◽  
Yuji Nishizawa ◽  
Arnoud Sonnenberg ◽  
Katsushi Owaribe
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Type Ii ◽  
Mammary Gland Epithelial Cell

Isolation, characterization, and EGFP expression in the buffalo (Bubalus bubalis) mammary gland epithelial cell line

2012 ◽  
Vol 49 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Ramakant Kaushik ◽  
Karn Pratap Singh ◽  
Archana Kumari ◽  
Manoj Kumar Singh ◽  
Radhey Shyam Manik ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Bubalus Bubalis ◽  
Egfp Expression ◽  
Epithelial Cell Line ◽  
Mammary Gland Epithelial Cell

scholarly journals Establishment and Characterization of a Lactating Caprine Mammary Gland Luminal Epithelial Cell Line

2019 ◽  
Vol 11 (4) ◽  
pp. 36
Author(s):  
Mahipal Singh ◽  
Benjamin Hortman ◽  
Venkata Degala ◽  
Xiaoling Ma
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Cell Line ◽  
Milk Production ◽  
Cell Cultures ◽  
Epithelial Cell Line ◽  
Luminal Epithelial Cell ◽  
Saanen Goat

Mammary gland is a defining characteristic of mammalian species which produces nutritious milk and plays a major role in the development of newborns. The gland contains a series of ducts and crevices leading back to alveoli, which contain milk producing cells called luminal epithelial cells. These cells, if cultured in-vitro, can be utilized to explore the metabolic processes occurring during milk production. The knowledge thus gained can be used to manipulate the system to enhance milk production and/or modify its composition. The main objective of this study was to establish a luminal epithelial cell-line from a lactating goat. Explant culture technique was used to produce primary cells from the mammary tissue of a 4-year-old lactating Saanen goat. The outgrowing cells were purified by selective trypsinization to remove fibroblast cells in 3-4 serial passages. The purified cell cultures exhibited cobblestone morphology, typical of the mammary epithelial cells, formed clear islands when plated in low density, and exhibited dome-shaped structures, if cultured for extended time. The cells stained positive with anti-human cytokeratin 18 antibodies, confirming their epithelial nature. Cell cultures also stained positive with rabbit anti-bovine β-lactoglobulin antibodies, indicating milk production in these cells. The cell-line has potential as an in-vitro cell model to understand signaling during milk synthesis, mammary gland development, and testing DNA constructs for therapeutic protein secretion in milk, prior to production of transgenic goats.

282. Anti-apoptotic effects of glucocorticoids and progesterone in a human mammary epithelial cell line

2005 ◽  
Vol 17 (9) ◽  
pp. 118
Author(s):  
M. N. Berg ◽  
B. J. Waddell ◽  
A. M. Dharmarajan
Keyword(s):  
Mammary Gland ◽  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Human Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Human Mammary Epithelial ◽  
Apoptotic Effects

Previous studies in the lactating rat mammary gland have shown that removal of both progesterone and glucocorticoids can induce apoptosis, and that replacement with either steroid alone can prevent this effect.1 These results raised the possibility that progesterone may exert its anti-apoptotic effects in the mammary gland via the glucocorticoid receptor (GR). A similar mechanism has previously been proposed to account for the action of progesterone in the corpus luteum.2 To assess this possibility we examined the effects of glucocorticoids and progesterone on apoptosis in the non-tumorigenic human mammary epithelial cell line, MCF-10A. Initial PCR analysis demonstrated that MCF-10A cells do not express progesterone receptor mRNA (PR-A or PR-B). MCF-10A cells were incubated in serum-free DMEM:F12 medium with additives in the presence or absence of cortisol (10–6M) or progesterone (10–5M to 10–8M). DNA fragmentation (an index of apoptosis) was quantitated by either 3′-end labelling or by a novel method of image analysis of SYBR® gold-stained gels. DNA fragmentation was clearly increased after 24 h in the absence of cortisol, consistent with previous reports for this cell line.3 In a separate experiment, replacement of cortisol with progesterone also reduced apoptosis by 48 h, although this protection was evident only at the two highest progesterone doses (10–5M and 10–6M). These results suggest that despite the absence of a progesterone receptor, progesterone is able to prevent apoptosis in MCF-10A cells, albeit at a physiologically high doses. This supports the hypothesis that some actions of progesterone may be mediated via the GR. Further studies are required to determine the precise molecular pathways by which cortisol and progesterone prevent apoptosis. (1)Berg MN et al. (2002) Endocrinology 143, 222–227.(2)Sugino N et al. (1997) Endocrinology 138, 4497–4500.(3)Moran TJ et al. (2000) Cancer Res 60, 867–872.

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