scholarly journals Evaluation of Once- Versus Twice-Weekly Transvaginal Ultrasound-Guided Follicular Oocyte Aspiration With or Without FSH Stimulation from the Same Cows.

1995 ◽  
Vol 41 (4) ◽  
pp. 303-309 ◽  
Author(s):  
Kazufumi GOTO ◽  
Yasuyuki TANIMOTO ◽  
Wataru FUJII ◽  
Shinichiro TANIGUCHI ◽  
Kazuhisa TAKESHITA ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Ultrasound Guided ◽  
Oocyte Aspiration

scholarly journals Transvaginal ultrasound-guided oocyte aspiration for production of embryos <i>in vitro</i>

2002 ◽  
Vol 45 (1) ◽  
pp. 99-108
Author(s):  
J. A. Carter ◽  
S. Bellow ◽  
M. Meintjes ◽  
O. Perez ◽  
E. Ferguson ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Farm Animals ◽  
Embryo Research ◽  
Ultrasound Guided ◽  
Cyclic Activity ◽  
Embryo Production ◽  
Early Postpartum ◽  
In Vitro Embryo Production ◽  
Oocyte Aspiration

Abstract. reproductive potential in genetically valuable animals (BEAL et al., 1992). Now that repeatable oocyte retrieval methods are being fine-tuned, it is likely these procedures will become routinely used to obtain oocytes for further gamete and embryo research and also by seedstock producers for in vitro embryo production from farm animals in the commercial sector. The use of transvaginal ultrasound-guided oocyte aspiration and IVF procedure does offer an alternative to cattle producers who have genetically valuable cows that for some reason are unable to produce viable embryos through standard embryo collection procedures. This technology can be used on oocytes harvested from older ovulating or nonovulating cows, females with physical injuries (e.g., fractured leg) and problem cows having an abnormal cervix. Good success has been reported using IVF procedures on oocytes obtained from supplemental follicles of cows with cystic ovarian disease. With IVF the potential exists for more embryos to be produced in a shorter period of time, since the procedure can be repeated on the same cow 3 to 4 times or more a month. At this station, we are harvesting oocytes from early postpartum (< 40 days) beef and dairy cattle, before the female begins cyclic activity. The approach allows the opportunity to produce one or more extra calves from the cow before she is mated for a natural pregnancy. Currently, transvaginal ultrasound-guided oocyte aspiration is now being used to harvest valuable oocytes from minor farm animal breeds, from domestic females representing rare bloodlines, clinically infertile females and reproductively senescent cows. Research continues to find applications for this technology, including harvesting oocytes from young prepubertal heifers and early postpartum beef cows for in vitro embryo production. The use of ultrasound-guided oocyte aspiration should not be overlooked to obtain oocytes for in vitro embryo production and to aid in germplasm preservation of endangered exotic species.

368 OOCYTE ASPIRATION FROM TRANSITIONAL AND CYCLING MARES TREATED WITH eFSH

2006 ◽  
Vol 18 (2) ◽  
pp. 291
Author(s):  
S. H. Purcell ◽  
T. K. Suh ◽  
G. E. Seidel Jr
Keyword(s):  
Transvaginal Ultrasound ◽  
Animal Health ◽  
Ultrasound Guided ◽  
Control Groups ◽  
Short Term ◽  
Double Lumen ◽  
Once Daily ◽  
Oocyte Recovery ◽  
Oocyte Donors ◽  
Oocyte Aspiration

A major obstacle in studying equine oocyte maturation is a source of oocytes. The aim of this study was to determine if oocyte recovery following transvaginal ultrasound-guided aspirations could be improved by using eFSH. Fourteen mares ranging from 3 to 16 years of age were used as oocyte donors during the transitional (March to April) and cycling (May to June) seasons. Each follicle was aspirated with a 12-gauge double-lumen needle using a pressure of 150 mmHg and then flushed eight times using PBS supplemented with 10 U/mL heparin and 0.05% BSA. Large follicles (>20 mm) were aspirated first, followed by small follicles (10–20 mm). The first aspiration in each season was used to deplete the follicular pool of all follicles >10 mm; these data were not considered. Following this initial aspiration, mares were subjected to four rounds of aspirations every 10 to 11 days. In each round during the transitional season, half the mares received 12.5 mg eFSH (Bioniche Animal Health, Belleville, Ontario, Canada) once daily for four days prior to aspiration. For each round during the cycling season, half the mares received 12.5 mg eFSH twice daily for three days prior to aspiration. In each replicate, mares were alternated from treated to control groups during both seasons. Oocyte recovery and superstimulatory responses were analyzed using the GLM procedure of SAS (SAS Institute, Inc., Cary, NC, USA). During both the transitional and the cycling season, treatment with eFSH did not have a significant effect (P > 0.1) on the number of small or large follicles present at the time of aspiration (Table 1). For both seasons and size classes, the number of follicles varied by mare (P < 0.02). Oocyte recovery from small follicles during the transitional season improved during the later two rounds of aspiration (0.58 vs. 1.49; P < 0.01) and was also affected by mare (P = 0.06). During the cycling season, recovery of oocytes from both small and large follicles varied by mare (P < 0.05). Treatment with eFSH increased the number of oocytes recovered from large follicles in cycling mares (P < 0.05). This study indicates that responses to short-term eFSH treatment for the purpose of oocyte recovery vary greatly by mare. While treatment with eFSH did not increase the number of follicles available for aspiration (P > 0.1), it did increase the number of oocytes recovered from large follicles during the cycling season. Table 1. Oocyte recovery and superstimulatory response of mares treated with eFSH

scholarly journals Retrospective analysis of transvaginal ultrasound-guided aspiration of simple ovarian cysts

2019 ◽  
Vol 28 (11) ◽  
pp. 1531-1535
Author(s):  
Marta Kostrzewa ◽  
Agnieszka Zając ◽  
Jacek Wilczyński ◽  
Grzegorz Stachowiak
Keyword(s):  
Retrospective Analysis ◽  
Transvaginal Ultrasound ◽  
Ovarian Cysts ◽  
Ultrasound Guided
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