scholarly journals Birth of Calves Derived from Oocytes Collected by Transvaginal Ultrasound Guided Follicular Aspiration.

1995 ◽  
Vol 41 (2) ◽  
pp. 171-174 ◽  
Author(s):  
Kazufumi GOTO ◽  
Yasuaki TANIMOTO ◽  
Shoji OOKUTSU ◽  
Yoshihiko NAKANISHI ◽  
Koichi YANAGITA ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Ultrasound Guided ◽  
Follicular Aspiration

scholarly journals Limitations of ultrasound guided follicular aspiration for analysis of ovarian follicular fluid in dairy cattle

2011 ◽  
Vol 80 (2) ◽  
pp. 179-184 ◽  
Author(s):  
Svatopluk Čech ◽  
Eva Indrová ◽  
Miloslava Lopatářová ◽  
Jana Malá ◽  
Alena Pechová ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Transvaginal Ultrasound ◽  
Ovarian Cysts ◽  
Ultrasound Guided ◽  
Acid Base Balance ◽  
Acid Base ◽  
New Device ◽  
Follicular Aspiration ◽  
Analysis Experiment ◽  
Base Balance

The aim of this study was to evaluate the applicability of ovum pick-up equipment for follicular fluid collection from various follicular structures (experiment 1) and for recovery of follicular fluid for acid-base balance analysis (experiment 2). An ultrasound scanner equipped with a 5-MHz convex transducer was used for transvaginal ultrasound-guided follicular aspiration. A 17-gauge, 60-cm aspiration needle was connected with a shortened aspiration line. The fluid was aspirated manually into a 2 ml plastic syringe at a speed of approximately 0.2 ml/s. The success of aspiration was higher in ovarian cysts (100%) and single follicles larger than 13 mm (76.7%) compared to single follicles smaller than 12 mm (20%,p< 0.001). The success of aspiration of multiple follicles on day 4 (diameter of 7–9 mm) was higher (90.9%) compared to follicles on day 2 (diameter of 4-6 mm) (66.7%,p< 0.05) in experiment 1. The fluid from ovarian cysts > 25 mm in diameter was aspirated in a two-step procedure (samples 1 and 2) for the determination of pH, HCO3,BE, pCO2and pO2(experiment 2). The indicators were compared between samples 1 and 2. Higher pO2as well as pH and lower pCO2in sample 1 compared to sample 2 showed insufficient anaerobic conditions during the first phase of the puncture in experiment 2. Our study brings for the first time the finding that the ovum pick-up equipment used in our experiments is suitable for the collection of follicular fluid only from larger follicular structures. The sampling of follicular fluid for acid-base balance assays requires the development of a special new device to prevent samples from coming into contact with air during aspiration.

Transvaginal ultrasound-guided follicular aspiration of equine oocytes

2010 ◽  
Vol 25 (S15) ◽  
pp. 71-74 ◽  
Author(s):  
N. L. COOK ◽  
E. L. SQUIRES ◽  
B. S. RAY ◽  
D. J. JASKO
Keyword(s):  
Transvaginal Ultrasound ◽  
Ultrasound Guided ◽  
Follicular Aspiration

scholarly journals In vivo ultrasound guided transvaginal oocyte collection in the cow

2018 ◽  
Vol 49 (3) ◽  
pp. 195
Author(s):  
G. S. AMIRIDIS (Γ.Σ. ΑΜΟΙΡΙΔΗΣ) ◽  
M. SALAHEDDINE ◽  
I. A. JEFFCOATE ◽  
E. VAINAS (Ε. ΒΑΪΝΑΣ) ◽  
L. ROBERTSON
Keyword(s):  
Recovery Rate ◽  
Transvaginal Ultrasound ◽  
Oestrous Cycle ◽  
Ultrasound Guided ◽  
Follicular Aspiration ◽  
Oocyte Recovery ◽  
Oocyte Collection ◽  
Significant Difference

This paper describes the results of the in vivo ultrasound guided follicular aspiration for ovum pick υρ (OPU) in the cow. Twelve non pregnant dry cows aged 4-6 years were used in this experiment. Eight cows underwent OPU during three successive oestrous cycles and another four cows were used as controls having only transvaginal ultrasound scanning of their ovaries. Oocyte collection took place three times during the luteal phase of each natural oestrous cycle (days 3-4,9-11 and 14-17). The content of 326 follicles with a diameter of 4-15mm was aspirated and 104 oocytes were collected (recovery rate 31.9% or 1.55 oocytes per cow and session). The oocyte recovery rate increased after the first three sessions (from 13.04% to 35.0%) and reached levels of υρ to 52.6%. More follicles were aspirated on days 9-11 (133 follicles 40.8%) compared to 111 (34%) follicles on days 14-17 and 82 (25%) on days 3-4) (P<0.05). The evaluation of the collected oocytes revealed that 60 oocytes (57.7%) were suitable for further in vitro manipulation. Neither the origin of the oocyte (left or right ovary) nor the stage of the oestrous cycle affected the recovery rate or the quality of the collected oocytes. There was no significant difference either in the length of the oestrous cycle between the experimental animals and the controls (21.6± 1.4 vs. 22.37±1.0 respectively), or in plasma progesterone concentration in daily collected blood samples from the animals of the two groups. The results of this study are compared to those from the international literature and to the results from endoscopical methods for oocyte recovery. The feasibility of application of this technique to projects designed to improve the genetic merit of cows is discussed.

Technical aspects of transvaginal ultrasound-guided follicular aspiration in cows

1994 ◽  
Vol 134 (17) ◽  
pp. 440-443 ◽  
Author(s):  
C. Scott ◽  
L. Robertson ◽  
R. de Moura ◽  
C. Paterson ◽  
J. Boyd
Keyword(s):  
Transvaginal Ultrasound ◽  
Ultrasound Guided ◽  
Technical Aspects ◽  
Follicular Aspiration

In vitro embryo production from bovine oocyte donors aspirated at different frequencies or following treatment with FSH

1996 ◽  
Vol 1996 ◽  
pp. 68-68
Author(s):  
K.L. Goodhand ◽  
R.G. Watt ◽  
M.E. Staines ◽  
L.C. Higgins ◽  
P.J. Broadbent ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Genetic Change ◽  
Bovine Oocyte ◽  
Ultrasound Guided ◽  
Embryo Production ◽  
Follicular Aspiration ◽  
Oocyte Donors ◽  
Pre Treatment

The combination of in vivo recovery of oocytes using transvaginal ultrasound guided aspiration and subsequent in vitro embryo production can be used to increase the rate of genetic change for efficiency of beef production by increasing selection intensity and reducing generation interval. The total number of oocytes recovered by aspiration and embryos produced is directly proportional to the number of aspiration sessions whether recovery takes place once or twice weekly. Pre-treatment of oocyte donors with FSH has been shown to improve the number of follicles available for aspiration but effects on embryo production have been conflicting (Bungartz et al., 1995; Goodhand et al., in press). The objective of this experiment was to compare the effect on embryo production of frequency of follicular aspiration and pre-treatment of donor cattle with FSH.

Transvaginal ultrasound guided follicular aspiration of bovine oocytes

1991 ◽  
Vol 35 (1) ◽  
pp. 19-24 ◽  
Author(s):  
M.C. Pieterse ◽  
P.L.A.M. Vos ◽  
Th.A.M. Kruip ◽  
Y.A. Wurth ◽  
Th.H. van Beneden ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Ultrasound Guided ◽  
Follicular Aspiration ◽  
Bovine Oocytes

Transvaginal ultrasound guided follicular aspiration of bovine oocytes

1991 ◽  
Vol 35 (4) ◽  
pp. 857-862 ◽  
Author(s):  
M.C. Pieterse ◽  
P.L.A.M. Vos ◽  
Th.A.M. Kruip ◽  
Y.A. Wurth ◽  
Th.H. van Beneden ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Ultrasound Guided ◽  
Follicular Aspiration ◽  
Bovine Oocytes

scholarly journals Comparing transabdominal and transvaginal ultrasound-guided follicular aspiration: A risk assessment formula

2015 ◽  
Vol 54 (6) ◽  
pp. 693-699 ◽  
Author(s):  
Christian F. Roman-Rodriguez ◽  
Ellerie Weissbrot ◽  
Chaur-Dong Hsu ◽  
Ashley Wong ◽  
Caleb Siefert ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Transvaginal Ultrasound ◽  
Ultrasound Guided ◽  
Follicular Aspiration

In vitro embryo production from bovine oocyte donors aspirated at different frequencies or following treatment with FSH

1996 ◽  
Vol 1996 ◽  
pp. 68-68
Author(s):  
K.L. Goodhand ◽  
R.G. Watt ◽  
M.E. Staines ◽  
L.C. Higgins ◽  
P.J. Broadbent ◽  
...  
Keyword(s):  
Transvaginal Ultrasound ◽  
Genetic Change ◽  
Bovine Oocyte ◽  
Ultrasound Guided ◽  
Embryo Production ◽  
Follicular Aspiration ◽  
Oocyte Donors ◽  
Pre Treatment

The combination of in vivo recovery of oocytes using transvaginal ultrasound guided aspiration and subsequent in vitro embryo production can be used to increase the rate of genetic change for efficiency of beef production by increasing selection intensity and reducing generation interval. The total number of oocytes recovered by aspiration and embryos produced is directly proportional to the number of aspiration sessions whether recovery takes place once or twice weekly. Pre-treatment of oocyte donors with FSH has been shown to improve the number of follicles available for aspiration but effects on embryo production have been conflicting (Bungartz et al., 1995; Goodhand et al., in press). The objective of this experiment was to compare the effect on embryo production of frequency of follicular aspiration and pre-treatment of donor cattle with FSH.

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