scholarly journals Immunohistochemical Studies of Insulin-Like Growth Factor-I in Corpora Lutea of Cycling and Pregnant Mice, Rats, Hamsters, Guinea Pigs and Cattle.

1994 ◽  
Vol 40 (2) ◽  
pp. 79-84 ◽  
Author(s):  
Hiromichi TAMADA ◽  
Mika OHNO ◽  
Tsutomu SAWADA ◽  
Junichi MORI
Keyword(s):  
Growth Factor ◽  
Guinea Pigs ◽  
Corpora Lutea ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Pregnant Mice ◽  
Growth Factor I ◽  
Immunohistochemical Studies

Blended Effects of Herbal Components of Tokishakuyakusan on Somatomedin C/Insulin-like Growth Factor 1 Level in Rat Corpus Luteum

1991 ◽  
Vol 19 (01) ◽  
pp. 61-64 ◽  
Author(s):  
Satoshi Usuki
Keyword(s):  
Growth Factor ◽  
Corpus Luteum ◽  
Corpora Lutea ◽  
Insulin Like Growth Factor ◽  
Somatomedin C ◽  
Factor I ◽  
Peony Root ◽  
Growth Factor I ◽  
Herbal Components

The effect of herbal components of Tokishakuyakusan on somatomedin C/insulin-like growth factor I (IGF-1) level in medium from rat corpora lutea incubated in vitro was examined. Hoelen + peony root + Japanese angelica root, hoelen + peony root, hoelen + Japanese angelica root or peony root + Japanese angelica root decreased the IGF-1 level. The data suggest that constituent herbal components of Tokishakuyakusan regulate the IGF-1 level by rat corpora lutea.

Late pregnancy increases hepatic expression of insulin-like growth factor-I in well nourished guinea pigs

2005 ◽  
Vol 15 (2) ◽  
pp. 165-171 ◽  
Author(s):  
P.A. Grant ◽  
K.L. Kind ◽  
C.T. Roberts ◽  
A. Sohlstrom ◽  
P.C. Owens ◽  
...  
Keyword(s):  
Growth Factor ◽  
Guinea Pigs ◽  
Late Pregnancy ◽  
Insulin Like Growth Factor ◽  
Hepatic Expression ◽  
Factor I ◽  
Growth Factor I

Effects of passive immunization of growing guinea-pigs with an insulin-like growth factor-I monoclonal antibody

1990 ◽  
Vol 124 (3) ◽  
pp. 403-415 ◽  
Author(s):  
D. E. Kerr ◽  
B. Laarveld ◽  
J. G. Manns
Keyword(s):  
Monoclonal Antibody ◽  
Growth Factor ◽  
Guinea Pigs ◽  
Treated Group ◽  
Insulin Like Growth Factor ◽  
Local Production ◽  
Factor I ◽  
Igf I ◽  
Animal Growth ◽  
Growth Factor I

ABSTRACT The physiological importance of circulating as opposed to locally produced insulin-like growth factor-I (IGF-I) has not been determined. By using a passive immunoneutralization technique, our objectives were to evaluate the role of circulating IGF-I in the regulation of animal growth and pituitary GH content. A monoclonal antibody (MAb) to IGF-I, generated in our laboratory, has an affinity (Ka) of 0·13 litres/pmol for recombinant human IGF-I (rhIGF-I). Cross-reactivities of recombinant des-tripeptide IGF-I and recombinant bovine IGF-II were approximately 40 and 8% respectively. This MAb inhibited binding of purified hIGF-I to human placental membranes. In a radioimmunoassay based on displacement of 125I-labelled rhIGF-I from the MAb, displacement curves generated with dilutions of acid–gel chromatography extracts of guinea-pig serum and rhIGF-I standards were parallel. Twenty-four, 3-week-old male guinea-pigs were treated with the IGF-I MAb, a bovine herpes virus-I (BHV-I) MAb (control MAb) or vehicle (phosphate-buffered saline) (n = 8 per group). Treatments were administered i.p. every 3 days for 24 days at a dose of 20 mg/kg body weight. Blood was obtained on day 23 (48 h after treatment) and on day 25 (24 h after treatment). In a liquid-phase assay, serum from the IGF-I MAb-treated group bound 38 ± 8% (mean ± s.e.m.) (day 23) and 56 ± 7% (day 25) of an 125I-labelled rhIGF-I trace at a final dilution of 1:10 000. Because of the development of an anti-mouse immune response in the guinea-pigs, these parameters would probably have been much greater during the first 2 weeks of the trial. Of the total IGF-I in serum, 50 ± 5% and 61±4% could be immunoprecipitated with an excess of rabbit anti-mouse immunoglobulin in samples from days 23 and 25 respectively. Comparisons between the groups treated with IGF-I MAb and BHV-I MAb revealed no significant differences in whole animal growth rate, growth of individual tissues, or pituitary GH content. Mean serum concentrations of IGF-I were 69 and 99% greater in IGF-I MAb-treated group than in the BHV-I MAb-treated group on days 23 and 25 respectively. These differences probably resulted from an extension of the half-life of IGF-I in serum of animals treated with the IGF-I MAb. The lack of effect of treatment with the IGF-I MAb suggests that local production of IGF-I is generally sufficient to maintain normal growth or that local production or activity of IGF-I is increased in a compensatory fashion. Journal of Endocrinology (1990) 124, 403–415

scholarly journals Steroidogenic responses of pig corpora lutea to insulin-like growth factor I (IGF-I) throughout the oestrous cycle

Reproduction ◽  
2003 ◽  
pp. 241-249 ◽  
Author(s):  
EA Miller ◽  
Z Ge ◽  
V Hedgpeth ◽  
JE Gadsby
Keyword(s):  
Signal Transduction ◽  
Growth Factor ◽  
Oestrous Cycle ◽  
Corpora Lutea ◽  
Insulin Like Growth Factor ◽  
Luteal Cells ◽  
Factor I ◽  
Progesterone Secretion ◽  
Igf I ◽  
Growth Factor I

This study was designed to investigate the roles of insulin-like growth factor I (IGF-I), IGF-type I receptor (IGF-IR) and IGF-binding proteins (IGFBPs) in regulating progesterone secretion by pig corpora lutea during the oestrous cycle, and the signal transduction pathways involved in mediating the steroidogenic actions of IGF-I. Corpora lutea were collected on days 4, 7, 10, 13 and 15 or 16 of the oestrous cycle, enzyme dissociated and the luteal cells were cultured for 24 h in Medium 199 with IGF-I (0-100 ng ml(-1)), long R(3)-IGF-I (0-100 ng ml(-1)), anti-IGF-I (Sm 1.2B; 0-10 microg ml(-1)), anti-IGF-IR (alphaIR3; 0-2 microg ml(-1)), or IGF-I signal transduction pathway inhibitors (phosphatidylinositol (PI)-3-kinase: 100 nmol Wortmannin l(-1) and 10 micromol LY 294002 l(-1); MAP kinase: 50 micromol PD 98059 l(-1)) to investigate their effects on IGF-I (100 ng ml(-1)) stimulated progesterone secretion. Pig luteal cells displayed dose-dependent responses to IGF-I and long R(3)-IGF-I on days 4 and 7 of the oestrous cycle, but not on days 10-16. There was no difference in the ED(50) or V(max) (maximal response) values between IGF-I and long R(3)-IGF-I. Neither anti-IGF-I nor anti-IGF-IR had significant effects on progesterone secretion, at any dose or day. Wortmannin and LY 294002 blocked IGF-I stimulated progesterone secretion, but PD 98059 was without effect. Finally, IGF-I (6 microg) infused into the ovary on day 7 in vivo significantly increased progesterone secretion within 45 min of infusion. The conclusions of this study are: (1) IGF-I has steroidogenic actions only on 'young' (days 4-7) pig corpora lutea; (2) endogenous IGF-I and IGFBP are insufficient to modulate progesterone secretion in vitro; and (3) the steroidogenic actions of IGF-I are mediated via PI-3-kinase.

Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture

1988 ◽  
Vol 116 (1) ◽  
pp. 97-100 ◽  
Author(s):  
D. Schams ◽  
R. Koll ◽  
C. H. Li
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Corpora Lutea ◽  
Dependent Manner ◽  
Insulin Like Growth Factor ◽  
Luteal Cells ◽  
Factor I ◽  
Igf I ◽  
Epidermal Growth ◽  
Growth Factor I

ABSTRACT The effect of insulin-like growth factor-I (IGF-I), epidermal growth factor (EGF), fibroblast growth factor (FGF) and nerve growth factor (NGF) on production of oxytocin and progesterone by cultured bovine granulosa and luteal cells was studied. Secretion of oxytocin was stimulated, in a dose-dependent manner, by IGF-I at 48 and 120 h of culture to levels much higher than those after stimulation with LH, FSH, EGF, FGF or NGF. A similar effect of IGF-I was observed for progesterone but, in contrast to oxytocin, secretion of progesterone was not increased by EGF, NGF or FGF. During primary culture, for 4 h, of dispersed bovine luteal cells obtained from corpora lutea between days 4 and 10 of the oestrous cycle, all the growth factors tested failed to stimulate secretion of oxytocin or progesterone. The data suggest the relevance of growth factors (especially IGF-I) for ovarian physiology and their possible importance for differentiation of follicles and luteinization. J. Endocr. (1988) 116, 97–100

scholarly journals Insulin-like growth factor I receptor mRNA and protein expression in pig corpora lutea

Reproduction ◽  
2000 ◽  
Vol 120 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Z Ge ◽  
W. Nicholson ◽  
D. Plotner ◽  
C. Farin ◽  
J. Gadsby
Keyword(s):  
Growth Factor ◽  
Protein Expression ◽  
Corpora Lutea ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Receptor Mrna ◽  
Mrna And Protein Expression ◽  
Growth Factor I
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