scholarly journals Ultrastructural comparison of porcine putative embryonic stem cells derived by in vitro fertilization and somatic cell nuclear transfer

2016 ◽  
Vol 62 (2) ◽  
pp. 177-185 ◽  
Author(s):  
Hyunju YOO ◽  
Eunhye KIM ◽  
Seon-Ung HWANG ◽  
Junchul David YOON ◽  
Yubyeol JEON ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem

scholarly journals Compromised Chondrocyte Differentiation Capacity in TERC Knockout Mouse Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer

2019 ◽  
Vol 20 (5) ◽  
pp. 1236 ◽  
Author(s):  
Wei-Fang Chang ◽  
Yun-Hsin Wu ◽  
Jie Xu ◽  
Li-Ying Sung
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Population Doubling ◽  
Chondrocyte Differentiation ◽  
Wild Type

Mammalian telomere lengths are primarily regulated by telomerase, consisting of a reverse transcriptase protein (TERT) and an RNA subunit (TERC). We previously reported the generation of mouse Terc+/− and Terc−/− embryonic stem cells (ntESCs) by somatic cell nuclear transfer. In the present work, we investigated the germ layer development competence of Terc−/−, Terc+/− and wild-type (Terc+/+) ntESCs. The telomere lengths are longest in wild-type but shortest in Terc−/− ntESCs, and correlate reversely with the population doubling time. Interestingly, while in vitro embryoid body (EB) differentiation assay reveals EB size difference among ntESCs of different genotypes, the more stringent in vivo teratoma assay demonstrates that Terc−/− ntESCs are severely defective in differentiating into the mesodermal lineage cartilage. Consistently, in a directed in vitro chondrocyte differentiation assay, the Terc−/− cells failed in forming Collagen II expressing cells. These findings underscore the significance in maintaining proper telomere lengths in stem cells and their derivatives for regenerative medicine.

A Novel Method for Somatic Cell Nuclear Transfer to Mouse Embryonic Stem Cells

2005 ◽  
Vol 7 (4) ◽  
pp. 265-271 ◽  
Author(s):  
Danièle Pralong ◽  
Krzysztof Mrozik ◽  
Filomena Occhiodoro ◽  
Nishanthi Wijesundara ◽  
Huseyin Sumer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Novel Method

Producing primate embryonic stem cells by somatic cell nuclear transfer

Nature ◽  
2007 ◽  
Vol 450 (7169) ◽  
pp. 497-502 ◽  
Author(s):  
J. A. Byrne ◽  
D. A. Pedersen ◽  
L. L. Clepper ◽  
M. Nelson ◽  
W. G. Sanger ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem

scholarly journals Characterization of Tetraploid Somatic Cell Nuclear Transfer-Derived Human Embryonic Stem Cells

2017 ◽  
Vol 21 (4) ◽  
pp. 425-434
Author(s):  
Dong-Hyuk Shin ◽  
Jeoung-Eun Lee ◽  
Jin Hee Eum ◽  
Young Gie Chung ◽  
Hoon Taek Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Human Embryonic Stem Cells ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem

In vitro induction of natural killer T cells from embryonic stem cells prepared using somatic cell nuclear transfer

2008 ◽  
Vol 22 (7) ◽  
pp. 2223-2231 ◽  
Author(s):  
Hiroshi Wakao ◽  
Rika Wakao ◽  
Sakura Sakata ◽  
Kazuya Iwabuchi ◽  
Atsushi Oda ◽  
...  
Keyword(s):  
Stem Cells ◽  
T Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem ◽  
Natural Killer T Cells ◽  
Killer T Cells ◽  
In Vitro Induction

Bovine chimeric offspring produced by transgenic embryonic stem cells generated from somatic cell nuclear transfer embryos

1998 ◽  
Vol 49 (1) ◽  
pp. 236 ◽  
Author(s):  
J.B. Cibelli ◽  
S.L. Stice ◽  
J.J. Kane ◽  
P.G. Golueke ◽  
J. Jerry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem

scholarly journals Recombination Signatures Distinguish Embryonic Stem Cells Derived by Parthenogenesis and Somatic Cell Nuclear Transfer

2007 ◽  
Vol 1 (3) ◽  
pp. 346-352 ◽  
Author(s):  
Kitai Kim ◽  
Kitwa Ng ◽  
Peter J. Rugg-Gunn ◽  
Jae-Hung Shieh ◽  
Oktay Kirak ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Embryonic Stem
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