scholarly journals Pluripotent cell derivation from male germline cells by suppression of Dmrt1 and Trp53

2015 ◽  
Vol 61 (5) ◽  
pp. 473-484 ◽  
Author(s):  
Takashi TANAKA ◽  
Mito KANATSU-SHINOHARA ◽  
Michiko HIROSE ◽  
Atsuo OGURA ◽  
Takashi SHINOHARA
Keyword(s):  
Pluripotent Cell ◽  
Male Germline ◽  
Germline Cells

scholarly journals Comprehensive histochemical profiles of histone modification in male germline cells during meiosis and spermiogenesis: Comparison of young and aged testes in mice

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0230930 ◽  
Author(s):  
Misako Tatehana ◽  
Ryuichi Kimura ◽  
Kentaro Mochizuki ◽  
Hitoshi Inada ◽  
Noriko Osumi
Keyword(s):  
Histone Modification ◽  
Male Germline ◽  
Germline Cells

scholarly journals Ring canals permit extensive cytoplasm sharing among germline cells independent of fusomes inDrosophilatestes

2019 ◽  
Author(s):  
Ronit S. Kaufman ◽  
Kari L. Price ◽  
Katelynn M. Mannix ◽  
Kathleen Ayers ◽  
Andrew M. Hudson ◽  
...  
Keyword(s):  
Oocyte Growth ◽  
Minimal Impact ◽  
Daughter Cells ◽  
Male Germline ◽  
Ring Canals ◽  
Sperm Development ◽  
Intercellular Movement ◽  
Germline Cells ◽  
Endogenous Proteins ◽  
Leaving Groups

AbstractIntercellular bridges, also called ring canals (RCs), connect germline cells during gametogenesis in males and females. They form as a consequence of incomplete cytokinesis during cell division leaving groups of daughter cells connected in syncytia. InDrosophilafemales, RCs are required for oocyte growth but little is known about the role of RCs during spermatogenesis. Using live imaging, we document extensive intercellular movement of GFP and a subset of endogenous proteins through RCs during spermatogenesis from two-cell diploid spermatogonia to clusters of 64 post-meiotic haploid spermatids. Loss of the fusome, a large cytoplasmic structure extending through RCs that is known to be important during oogenesis, has minimal impact on RC development or intercellular protein movement during spermatogenesis. Our results reveal that male germline RCs remain persistently open and mediate extensive sharing of cytoplasmic information, supporting multiple roles for RCs throughout sperm development.

scholarly journals SPATA33 is an autophagy mediator for cargo selectivity in germline mitophagy

2020 ◽  
Author(s):  
Ying Zhang ◽  
Xu Xu ◽  
Mengxin Hu ◽  
Xin Wang ◽  
Hanhua Cheng ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Mitochondrial Membrane ◽  
Outer Mitochondrial Membrane ◽  
Autophagosome Formation ◽  
Cellular Homeostasis ◽  
Male Germline ◽  
Autophagic Degradation ◽  
Carboxyl Terminal ◽  
Germline Cells ◽  
Mitochondrial Membrane Protein

Abstract Selective autophagic degradation of mitochondria (mitophagy) is important in maintaining proper cellular homeostasis. Here, we found that SPATA33 is a novel autophagy mediator for mitophagy in testis. The SPATA33 protein localizes on mitochondria via its binding of the carboxyl terminal with the outer mitochondrial membrane protein VDAC2. Upon starvation induction, SPATA33 is recruited to autophagosome by binding the autophagy machinery ATG16L1 via its N-terminal along with mitochondria. Notably, Spata33 knockout inhibited autophagy and overexpression can promote autophagosome formation for mitochondrial sequestration. Therefore, SPATA33 confers selectivity for mitochondrial degradation and promotes mitophagy in male germline cells.

scholarly journals Male-biased protein expression in primordial germ cells, identified through a comparative study of UAS vectors in Drosophila

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masaki Masukawa ◽  
Yuki Ishizaki ◽  
Hiroki Miura ◽  
Makoto Hayashi ◽  
Ryoma Ota ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Expression ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Translational Activity ◽  
Male Germline ◽  
Germline Cells ◽  
Female Germline ◽  
Go Terms ◽  
Mrna Expression Levels

AbstractIn Drosophila, three types of UAS vectors (UASt, UASp, and UASz) are currently available for use with the Gal4-UAS system. They have been used successfully in somatic cells and germline cells from ovaries. However, it remains unclear whether they are functional in the germline cells of embryos, larvae, and adult testes. In this study, we found that all three types of UAS vectors were functional in the germline cells of embryos and larvae and that the UASt and UASz vectors were active in the germline of the distal tip region in adult testes. Moreover, we observed that protein expression from the UAS vectors was male-biased in germline cells of late embryos, whereas their respective mRNA expression levels were not. Furthermore, O-propargyl-puromycin (OPP) staining revealed that protein synthesis was male-biased in these germline cells. In addition, GO terms related to translation and ribosomal maturation were significantly enriched in the male germline. These observations show that translational activity is higher in male than in female germline cells. Therefore, we propose that male-biased protein synthesis may be responsible for the sex differences observed in the early germline.

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