scholarly journals Transgenic Pigs with Pancreas-specific Expression of Green Fluorescent Protein

2014 ◽  
Vol 60 (3) ◽  
pp. 230-237 ◽  
Author(s):  
Hitomi MATSUNARI ◽  
Toshihiro KOBAYASHI ◽  
Masahito WATANABE ◽  
Kazuhiro UMEYAMA ◽  
Kazuaki NAKANO ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Transgenic Pigs ◽  
Specific Expression ◽  
Green Fluorescent

High level Purkinje cell specific expression of green fluorescent protein in transgenic mice

2001 ◽  
Vol 115 (6) ◽  
pp. 455-464 ◽  
Author(s):  
Xulun Zhang ◽  
Stephan L. Baader ◽  
Feng Bian ◽  
Wolfgang Müller ◽  
John Oberdick
Keyword(s):  
Green Fluorescent Protein ◽  
Transgenic Mice ◽  
Purkinje Cell ◽  
Fluorescent Protein ◽  
Specific Expression ◽  
Green Fluorescent ◽  
High Level

Intestine-specific expression of green fluorescent protein-tagged cathepsin B: proof-of-principle experiments

2008 ◽  
Vol 389 (8) ◽  
Author(s):  
Kristina Mayer ◽  
Maria E. Iolyeva ◽  
Ulf Meyer-Grahle ◽  
Klaudia Brix
Keyword(s):  
Transcription Factor ◽  
Green Fluorescent Protein ◽  
Cathepsin B ◽  
Fluorescent Protein ◽  
Regulatory Elements ◽  
Egfp Expression ◽  
Reporter Protein ◽  
Specific Expression ◽  
Green Fluorescent ◽  
Proof Of Principle

Abstract We hypothesized that tissue-specific expression of cathepsin B-enhanced green fluorescent protein (CB-EGFP) can be driven by the A33-antigen promoter that contains positive cis-regulatory elements, including caudal-related homeobox (CDX) binding sites. The intestine-specific transcription factor Cdx1 is crucial for A33-antigen promoter activation and could thereby induce expression of CB-EGFP. This concept was tested by construction of the vector pA33-CathB-EGFP encoding CB-EGFP downstream of the A33-antigen promoter. Its Cdx1 dependence, as an indication of its intestine-specific expression, was tested in Cdx1-negative CHO-K1 cells. Cdx1 expression was achieved upon transfection with pCdx1-DsRed-Express and was indicated by red fluorescence of the simultaneously translated reporter protein. Immunolabeling with Cdx1-specific antibodies showed correct targeting of the transcription factor to its point of action in nuclei of transfected cells. Co-transfection experiments with plasmids pA33-CathB-EGFP and pCdx1-DsRed-Express confirmed the hypothesis that Cdx1 indeed activates CB-EGFP expression in a manner dependent on the A33-antigen promoter. Co-localization with compartment-specific markers and subcellular fractionation confirmed CB-EGFP trafficking along the expected route to endolysosomal compartments. Hence, the A33-antigen promoter represents a potent tool for induction of Cdx1-dependent CB-EGFP expression in vitro. Our proof-of-principle studies confirm the suitability of this approach in visualizing protease transport in Cdx1-positive tissues of the gastrointestinal tract.

Oct4-Enhanced Green Fluorescent Protein Transgenic Pigs: A New Large Animal Model for Reprogramming Studies

2011 ◽  
Vol 20 (9) ◽  
pp. 1563-1575 ◽  
Author(s):  
Monika Nowak-Imialek ◽  
Wilfried A. Kues ◽  
Bjoern Petersen ◽  
Andrea Lucas-Hahn ◽  
Doris Herrmann ◽  
...  
Keyword(s):  
Animal Model ◽  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Large Animal Model ◽  
Large Animal ◽  
Transgenic Pigs ◽  
Green Fluorescent

Germline-specific expression of the Oct-4/green fluorescent protein (GFP) transgene in mice

1999 ◽  
Vol 41 (6) ◽  
pp. 675-684 ◽  
Author(s):  
Tomomi Yoshimizu ◽  
Noriyuki Sugiyama ◽  
Mario De Felice ◽  
Young Ii Yeom ◽  
Kazuyuki Ohbo ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Specific Expression ◽  
Green Fluorescent

Cardiac specific expression of the green fluorescent protein during early murine embryonic development

FEBS Letters ◽  
1998 ◽  
Vol 440 (3) ◽  
pp. 370-376 ◽  
Author(s):  
M Fleischmann ◽  
W Bloch ◽  
E Kolossov ◽  
C Andressen ◽  
M Müller ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Embryonic Development ◽  
Fluorescent Protein ◽  
Specific Expression ◽  
Green Fluorescent

scholarly journals Generation of Transgenic Rats Expressing Green Fluorescent Protein in S-100β-Producing Pituitary Folliculo-Stellate Cells and Brain Astrocytes

Endocrinology ◽  
2007 ◽  
Vol 148 (4) ◽  
pp. 1518-1523 ◽  
Author(s):  
Eisuke Itakura ◽  
Kousuke Odaira ◽  
Kotaro Yokoyama ◽  
Marumi Osuna ◽  
Takahiko Hara ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Gene Promoter ◽  
Anterior Lobe ◽  
Transgenic Rats ◽  
Specific Expression ◽  
Sustentacular Cells ◽  
Intron 1 ◽  
S 100 ◽  
Green Fluorescent

Folliculo-stellate (FS) cells are known to act as sustentacular cells or scavenger cells in the anterior lobe. However, the precise function and origin of FS cells are still under discussion. Like brain astrocytes, FS cells contain S-100β protein, and FS cells can be detected immunocytochemically using antibodies for S-100β protein after fixation; however, living FS cells can not be detected. The generation of transgenic rats expressing green fluorescent protein (GFP) under the control of S-100β protein gene promoter may allow the detection of living FS cells, which may be an excellent tool for the study of FS cells. With the aim of generation of transgenic rats, we analyzed the promoter activity of the S-100β gene and found that intron 1 is important for cell-specific expression of the S-100β gene. Therefore, we obtained a DNA construct containing GFP gene under a part of the S-100 promoter with intron 1. We transfected the construct into rat embryos and succeeded in generating transgenic rats. The transgenic rats expressed GFP in FS cells specifically in the anterior lobe. GFP is also expressed in other known S-100β-expressing cells, i.e. brain astrocytes, adipocytes, and chondrocytes. We believe that the newly generated transgenic rats will provide a new approach for the study of FS cells and other S-100β protein-producing cells.

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