scholarly journals Functional Characterization of Double-knockout Mouse Sperm Lacking SPAM1 and ACR or SPAM1 and PRSS21 in Fertilization

2012 ◽  
Vol 58 (3) ◽  
pp. 330-337 ◽  
Author(s):  
Chong ZHOU ◽  
Woojin KANG ◽  
Tadashi BABA
Keyword(s):  
Knockout Mouse ◽  
Functional Characterization ◽  
Double Knockout ◽  
Mouse Sperm ◽  
Double Knockout Mouse

Electroencephalographic characterization of seizure activity in the synapsin I/II double knockout mouse

2011 ◽  
Vol 1383 ◽  
pp. 270-288 ◽  
Author(s):  
Lars Etholm ◽  
Henrik Lindén ◽  
Torsten Eken ◽  
Paul Heggelund
Keyword(s):  
Knockout Mouse ◽  
Seizure Activity ◽  
Synapsin I ◽  
Double Knockout ◽  
Double Knockout Mouse

scholarly journals Omics profiling identifies MAPK/ERK pathway as a gatekeeper of nephron progenitor metabolism

2021 ◽  
Author(s):  
Hyuk Nam Kwon ◽  
Kristen Kurtzeborn ◽  
Xing Jin ◽  
Bruno Reversade ◽  
Sunghyouk Park ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Mitogen Activated Protein Kinase ◽  
Metabolic Effects ◽  
Erk Pathway ◽  
Double Knockout ◽  
Nephron Progenitor ◽  
Progenitor Maintenance

Nephron endowment is defined by fetal kidney growth and it critically dictates renal health in adults. Despite the advances in understanding the molecular regulation of nephron progenitor maintenance, propagation, and differentiation, the causes for low congenital nephron count and contribution of basic metabolism to nephron progenitor regulation remain poorly studied. Here we have analyzed the metabolic effects that depend on and are triggered by the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway, which is an essential intracellular cascade required for nephron progenitor maintenance. Our combined approach utilizing LC/MS-based metabolomics and transcriptional profiling of MAPK/ERK-deficient cells identified 18 out of total 46 metabolites (38 untargeted and 8 targeted) that were down-regulated. These represent glycolysis, gluconeogenesis, pentose phosphate, glycine, and proline pathways among others. We focused our functional characterization of identified metabolites on pyruvate and proline. Use of in vitro kidney cultures revealed dosage-specific functions for pyruvate in not only controlling ureteric bud branching but also determining progenitor and differentiated (tip-trunk) cell identities. Our in vivo characterization of Pycr1/2 double knockout kidneys revealed functional requirement for proline metabolism in nephron progenitor maintenance. In summary, our results demonstrate that MAPK/ERK cascade regulates energy and amino acid metabolism in developing kidney where these metabolic pathways specifically regulate progenitor preservation.

scholarly journals 226: Genes or environment? A novel double knockout mouse model for fetal origins of autism study

2016 ◽  
Vol 214 (1) ◽  
pp. S135
Author(s):  
Hind N. Moussa ◽  
Baha M. Sibai ◽  
Sean C. Blackwell ◽  
David A. Fournie ◽  
Alejandra E. Ontiveros ◽  
...  
Keyword(s):  
Mouse Model ◽  
Knockout Mouse ◽  
Double Knockout ◽  
Fetal Origins ◽  
Knockout Mouse Model ◽  
Double Knockout Mouse

scholarly journals Micro-MRI phenotyping of a novel double-knockout mouse model of congenital heart disease

2010 ◽  
Vol 12 (Suppl 1) ◽  
pp. P1 ◽  
Author(s):  
Jon O Cleary ◽  
Karen McCue ◽  
Anthony N Price ◽  
Sarah Beddow ◽  
Roger J Ordidge ◽  
...  
Keyword(s):  
Congenital Heart Disease ◽  
Heart Disease ◽  
Mouse Model ◽  
Knockout Mouse ◽  
Congenital Heart ◽  
Double Knockout ◽  
Knockout Mouse Model ◽  
Double Knockout Mouse

The effect of the long term aspirin administration on the progress of atherosclerosis in apoE-/- LDLR-/- double knockout mouse

2010 ◽  
Vol 125 (3) ◽  
pp. 246-252 ◽  
Author(s):  
Y. Yamamoto ◽  
T. Yamashita ◽  
F. Kitagawa ◽  
K. Sakamoto ◽  
J.C. Giddings ◽  
...  
Keyword(s):  
Knockout Mouse ◽  
Double Knockout ◽  
Double Knockout Mouse

scholarly journals A Case Report of a Malignant Fibrous Histiocytoma in a T-cell Receptor β Chain and p53 Double-knockout Mouse

2011 ◽  
Vol 24 (4) ◽  
pp. 251-255
Author(s):  
Minoru Ando ◽  
Shoichi Kado ◽  
Tomo Suzuki ◽  
Ryota Yamamoto ◽  
Yuriko Nagata ◽  
...  
Keyword(s):  
Case Report ◽  
T Cell ◽  
T Cell Receptor ◽  
Malignant Fibrous Histiocytoma ◽  
Knockout Mouse ◽  
Fibrous Histiocytoma ◽  
Cell Receptor ◽  
Double Knockout ◽  
Double Knockout Mouse ◽  
Β Chain
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