scholarly journals Analysis of Genes Expressed During Porcine Fetal Pituitary Development by Suppressive Subtraction Hybridization

2007 ◽  
Vol 53 (5) ◽  
pp. 1087-1091 ◽  
Author(s):  
Jun TAKAHASHI ◽  
Takao SUSA ◽  
Takanobu SATO ◽  
Hiroko ASANO ◽  
Takako KATO ◽  
...  
Keyword(s):  
Subtraction Hybridization ◽  
Pituitary Development ◽  
Suppressive Subtraction Hybridization

scholarly journals Functional Annotation of Hypothetical Proteins Derived from Suppressive Subtraction Hybridization (SSH) Analysis Shows NPR1 (Non-Pathogenesis Related)-Like Activity

Agronomy ◽  
2019 ◽  
Vol 9 (2) ◽  
pp. 57
Author(s):  
Murugesan Chandrasekaran ◽  
Chandrasekar Raman ◽  
Kandasamy Karthikeyan ◽  
Manivannan Paramasivan
Keyword(s):  
Protein Function ◽  
Functional Annotation ◽  
Rna Binding ◽  
Local Alignment ◽  
Banana Plant ◽  
Subtraction Hybridization ◽  
Atomic Contact ◽  
Suppressive Subtraction Hybridization ◽  
Pathogenesis Related Protein ◽  
Pathogenesis Related

Fusarium wilt is considered the most devastating banana disease incited by Fusarium oxysporum f. sp. cubense (FOC). The present study addresses suppressive subtraction hybridization (SSH) analysis for differential gene expression in banana plant, mediated through FOC and its interaction with biocontrol agent Trichoderma asperellum (prr2). SSH analysis yielded a total of 300 clones. The resultant clones were sequenced and processed to obtain 22 contigs and 87 singleton sequences. BLAST2GO (Basic Local Alignment Search Tool 2 Gene Ontology) analysis was performed to assign known protein function. Initial functional annotation showed that contig 21 possesses p38-like endoribonuclease activity and duality in subcellular localization. To gain insights into its additional roles and precise functions, a sequential docking protocol was done to affirm its role in the defense pathway. Atomic contact energies revealed binding affinities in the order of miRNA > phytoalexins > polyubiquitin, emphasizing their role in the Musa defense pathway. Contig 21 and polyubiquitin showed an atomic contact energy value of −479.60 kJ/mol, and even higher atomic contact energies were observed for miRNA (−804.86, −482.28, −494.75 kJ/mol), demonstrating its high RNA-binding properties. Phytoalexin contig 21-interacting interfacial residues were identified as rigid (10)/non-rigid (2) based on Bi, N values, and B-factor per residue. Hence, based on these results, contig 21 was characterized as a NPR1 (non-pathogenesis-related protein) homolog that is involved in plant defense and systemic induced resistance.

scholarly journals Suppressive Subtraction Hybridization on Stimulated Primary Horse Macrophages

2006 ◽  
Vol 75 (3) ◽  
pp. 337-341
Author(s):  
J. Matiašovic ◽  
P. Králík ◽  
L. Leva ◽  
M. Faldyna ◽  
P. Hořín
Keyword(s):  
Expressed Sequence Tags ◽  
Sequence Similarity ◽  
Genetic Resistance ◽  
Macrophage Cell ◽  
Cdna Subtraction ◽  
Subtraction Hybridization ◽  
E Coli ◽  
Suppressive Subtraction Hybridization ◽  
Expressed Sequence ◽  
Overnight Culture

To study genes potentially involved in genetic resistance to infectious diseases in the horse, suppressive subtraction hybridization was used to identify genes expressed in primary horse macrophages after their stimulation with E. coli. Overnight culture of blood monocyte-derived macrophage cells was stimulated with E. coli K12 in ratio 40 E. coli cells to one macrophage cell. After 4 hours of incubation, non-phagocyted bacteria were washed away. Following next 20 hour incubation in MEM alpha containing 5 μg of gentamycin in 1 ml of media, mRNA was isolated and used in Clontech PCR-Select cDNA Subtraction Kit. Expression of several known horse genes, as well as some new ESTs (expressed sequence tags) showing sequence similarity with immunity-related genes from other species was identified.

scholarly journals Identification of Male Gametogenesis Expressed Genes from the Scallop Nodipecten subnodosus by Suppressive Subtraction Hybridization and Pyrosequencing

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e73176 ◽  
Author(s):  
Raúl Llera-Herrera ◽  
Alejandra García-Gasca ◽  
Cei Abreu-Goodger ◽  
Arnaud Huvet ◽  
Ana M. Ibarra
Keyword(s):  
Subtraction Hybridization ◽  
Male Gametogenesis ◽  
Suppressive Subtraction Hybridization

Isolation of cold stress-responsive genes from Lepidium latifolium by suppressive subtraction hybridization

2009 ◽  
Vol 32 (1) ◽  
pp. 205-210 ◽  
Author(s):  
Mohammad Aslam ◽  
Vimlendu B. Sinha ◽  
Rupesh K. Singh ◽  
Sivalingam Anandhan ◽  
Veena Pande ◽  
...  
Keyword(s):  
Cold Stress ◽  
Lepidium Latifolium ◽  
Subtraction Hybridization ◽  
Suppressive Subtraction Hybridization ◽  
Stress Responsive Genes

Suppressive subtraction hybridization reveals that rice gall midge attack elicits plant-pathogen-like responses in rice

2013 ◽  
Vol 63 ◽  
pp. 122-130 ◽  
Author(s):  
Nidhi Rawat ◽  
Kudapa Himabindu ◽  
Chiruvuri Naga Neeraja ◽  
Suresh Nair ◽  
Jagadish S. Bentur
Keyword(s):  
Plant Pathogen ◽  
Gall Midge ◽  
Subtraction Hybridization ◽  
Suppressive Subtraction Hybridization ◽  
Rice Gall Midge
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