scholarly journals Spindle Formation and Microtubule Organization During First Division in Reconstructed Rat Embryos Produced by Somatic Cell Nuclear Transfer

2007 ◽  
Vol 53 (4) ◽  
pp. 835-842 ◽  
Author(s):  
Ikuo TOMIOKA ◽  
Eiji MIZUTANI ◽  
Tomoyuki YOSHIDA ◽  
Atsushi SUGAWARA ◽  
Kentaro INAI ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Microtubule Organization ◽  
Spindle Formation ◽  
Rat Embryos

Developmental Arrest and Cytoskeletal Anomalies of Rat Embryos Reconstructed by Somatic Cell Nuclear Transfer

2007 ◽  
Vol 9 (3) ◽  
pp. 382-393 ◽  
Author(s):  
Jae Gyu Yoo ◽  
Simon-Pierre Demers ◽  
Li Lian ◽  
Lawrence C. Smith
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Rat Embryos ◽  
Developmental Arrest

Treatment with MG132, a Proteasome Inhibitor, Through Somatic Cell Nuclear Transfer Improves Survival and Pronuclear Number of Reconstructed Rat Embryos.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 152-153
Author(s):  
Junya Ito ◽  
Noriaki Nakajima ◽  
Chika Suzukamo ◽  
Daisuke Sano ◽  
Shuhei Sugio ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Proteasome Inhibitor ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Rat Embryos

Development and spindle formation in rat somatic cell nuclear transfer (SCNT) embryos in vitro using porcine recipient oocytes

Zygote ◽  
2009 ◽  
Vol 17 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Atsushi Sugawara ◽  
Satoshi Sugimura ◽  
Yumi Hoshino ◽  
Eimei Sato
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer ◽  
Fluorescent Protein ◽  
Cumulus Cells ◽  
Blastocyst Stage ◽  
Developmental Competence ◽  
Spindle Formation ◽  
Fetal Fibroblasts

SummaryCloning that uses somatic cell nuclear transfer (SCNT) technology with gene targeting could be a potential alternative approach to obtain valuable rat models. In the present study, we determined the developmental competence of rat SCNT embryos constructed using murine and porcine oocytes at metaphase II (MII). Further, we assessed the effects of certain factors, such as: (i) the donor cell type (fetal fibroblasts or cumulus cells); and (ii) premature chromosome condensation (PCC) with normal spindle formation, on the developmental competence of rat interspecies SCNT (iSCNT) embryos. iSCNT embryos that had been constructed using porcine oocytes developed to the blastocyst stage, while those embryos made using murine MII oocytes did not. Rat iSCNT embryos constructed with green fluorescent protein (GFP)-expressing fetal fibroblasts injected into porcine oocytes showed considerable PCC with a normal bipolar spindle formation. The total cell number of iSCNT blastocyst derived from GFP-expressing fetal fibroblasts was higher than the number derived from cumulus cells. In addition, these embryos expressed GFP at the blastocyst stage. This paper is the first report to show that rat SCNT embryos constructed using porcine MII oocytes have the potential to develop to the blastocyst stage in vitro. Thus the iSCNT technique, when performed using porcine MII oocytes, could provide a new bioassay system for the evaluatation of the developmental competence of rat somatic cells.

scholarly journals Author Correction: Application of interspecific Somatic Cell Nuclear Transfer (iSCNT) in sturgeons and an unexpectedly produced gynogenetic sterlet with homozygous quadruple haploid

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Effrosyni Fatira ◽  
Miloš Havelka ◽  
Catherine Labbé ◽  
Alexandra Depincé ◽  
Viktoriia Iegorova ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Somatic Cell Nuclear Transfer ◽  
Nuclear Transfer
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