scholarly journals Effects of Iron and Copper in Culture Medium on Bovine Oocyte Maturation, Preimplantation Embryo Development, and Apoptosis of Blastocysts In Vitro

2007 ◽  
Vol 53 (4) ◽  
pp. 777-784 ◽  
Author(s):  
Guolong GAO ◽  
Jianming YI ◽  
Min ZHANG ◽  
Jiajun XIONG ◽  
Liying GENG ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Culture Medium ◽  
Preimplantation Embryo ◽  
Bovine Oocyte ◽  
Preimplantation Embryo Development

scholarly journals Endoplasmic Reticulum (ER) Stress and Unfolded Protein Response (UPR) in Mammalian Oocyte Maturation and Preimplantation Embryo Development

2019 ◽  
Vol 20 (2) ◽  
pp. 409 ◽  
Author(s):  
Tao Lin ◽  
Jae Lee ◽  
Jung Kang ◽  
Hyeon Shin ◽  
Ju Lee ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Signaling Pathways ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Preimplantation Embryo ◽  
Preimplantation Embryo Development ◽  
Unfolded Protein ◽  
Protein Response

Mammalian oocytes and early embryos derived from in vitro production are highly susceptible to a variety of cellular stresses. During oocyte maturation and preimplantation embryo development, functional proteins must be folded properly in the endoplasmic reticulum (ER) to maintain oocyte and embryo development. However, some adverse factors negatively impact ER functions and protein synthesis, resulting in the activation of ER stress and unfolded protein response (UPR) signaling pathways. ER stress and UPR signaling have been identified in mammalian oocytes and embryos produced in vitro, suggesting that modulation of ER stress and UPR signaling play very important roles in oocyte maturation and the development of preimplantation embryos. In this review, we briefly describe the current state of knowledge regarding ER stress, UPR signaling pathways, and their roles and mechanisms in mammalian (excluding human) oocyte maturation and preimplantation embryo development.

Hemoglobin: potential roles in the oocyte and early embryo†

2019 ◽  
Vol 101 (2) ◽  
pp. 262-270 ◽  
Author(s):  
Megan Lim ◽  
Hannah M Brown ◽  
Karen L Kind ◽  
Jeremy G Thompson ◽  
Kylie R Dunning
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Preimplantation Embryo ◽  
Cell Types ◽  
Cellular Function ◽  
Preimplantation Embryos ◽  
Low Oxygen ◽  
Preimplantation Embryo Development ◽  
Regulated Gene Expression

Abstract Hemoglobin (Hb) is commonly known for its capacity to bind and transport oxygen and carbon dioxide in erythroid cells. However, it plays additional roles in cellular function and health due to its capacity to bind other gases including nitric oxide. Further, Hb acts as a potent antioxidant, quenching reactive oxygen species. Despite its potential roles in cellular function, the preponderance of Hb research remains focused on its role in oxygen regulation. There is increasing evidence that Hb expression is more ubiquitous than previously thought, with Hb and its variants found in a myriad of cell types ranging from macrophages to spermatozoa. The majority of nonerythroid cell types that express Hb are situated within hypoxic environments, suggesting Hb may play a role in hypoxia-inducible factor-regulated gene expression by controlling the level of oxygen available or as an adaptation to low oxygen providing a mechanism to store oxygen. Oocyte maturation and preimplantation embryo development occur within the low oxygen environments of the antral follicle and oviduct/uterus, respectively. Interestingly, Hb was recently found in human cumulus and granulosa cells and murine cumulus–oocyte complexes and preimplantation embryos. Here, we consolidate and analyze the research generated todate on Hb expression in nonerythroid cells with a particular focus on reproductive cell types. We outline future directions of this research to elucidate the role of Hb during oocyte maturation and preimplantation embryo development and finally, we explore the potential clinical applications and benefits of Hb supplementation during the in vitro culture of gametes and embryos.

scholarly journals Role of Epidermal Growth Factor in Bovine Oocyte Maturation and Preimplantation Embryo Development in Vitro1

1996 ◽  
Vol 54 (6) ◽  
pp. 1420-1429 ◽  
Author(s):  
Pat Lonergan ◽  
Catherine Carolan ◽  
Anne Van Langendonckt ◽  
Isabelle Donnay ◽  
Hadj Khatir ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Embryo Development ◽  
Oocyte Maturation ◽  
Preimplantation Embryo ◽  
Bovine Oocyte ◽  
Preimplantation Embryo Development ◽  
Epidermal Growth

scholarly journals X-Linked Huwe1 Is Essential for Oocyte Maturation and Preimplantation Embryo Development

iScience ◽  
2020 ◽  
Vol 23 (9) ◽  
pp. 101523
Author(s):  
Alaa A. Eisa ◽  
Scott Bang ◽  
Katherine J. Crawford ◽  
Emily M. Murphy ◽  
William W. Feng ◽  
...  
Keyword(s):  
Embryo Development ◽  
Oocyte Maturation ◽  
Preimplantation Embryo ◽  
Preimplantation Embryo Development

The effect of perfluorohexanesulfonate (PFHxS) on bovine preimplantation embryo development in vitro

2021 ◽  
Vol 350 ◽  
pp. S169-S170
Author(s):  
I. Hallberg ◽  
M. Moberg ◽  
M. Olovsson ◽  
P. Damdimopoulou ◽  
J. Rüegg ◽  
...  
Keyword(s):  
Embryo Development ◽  
Preimplantation Embryo ◽  
Preimplantation Embryo Development ◽  
Development In Vitro

Effects of changing culture medium on preimplantation embryo development in rabbit

Zygote ◽  
2021 ◽  
pp. 1-6
Author(s):  
Haixia Wang ◽  
Wenbin Cao ◽  
Huizhong Hu ◽  
Chenglong Zhou ◽  
Ziyi Wang ◽  
...  
Keyword(s):  
Embryo Development ◽  
Embryo Culture ◽  
Culture Medium ◽  
Cell Number ◽  
Apoptotic Index ◽  
Total Cell ◽  
Toxic Substances ◽  
Total Cell Number ◽  
Preimplantation Embryo Development

Summary Many studies have focused on the optimization of the composition of embryo culture medium; however, there are few studies involving the effect of a culture medium changing procedure on the preimplantation development of embryos. In this study, three groups were designed: a non-renewal group, a renewal group and a half-renewal group. The levels of reactive oxygen species (ROS), apoptotic index, blastocyst ratio and blastocyst total cell number were analyzed in each group. The results showed that the ROS level and the apoptotic index of blastocyst in the non-renewal group were significantly higher than in the renewal group and the half-renewal group (P < 0.05). The blastocyst ratio and blastocyst total cell number were significantly higher in the half-renewal group than that in non-renewal group and the renewal group (P < 0.05). These results demonstrated that the procedure of changing the culture medium influenced ROS level, apoptotic index, blastocyst ratio and total cell number of blastocysts. In addition, the result suggested that changing the culture medium may lead to a loss of important regulatory factors for embryos, while not changing the culture medium may lead to the accumulation of toxic substances. Half-renewal can alleviate the defects of both no renewal and renewal, and benefit embryo development. This study will be of high value as a reference for the optimization of embryo culture in vitro, and is very significant for assisted reproduction.

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