scholarly journals Comparison of Glycerol, Lactamide, Acetamide and Dimethylsulfoxide as Cryoprotectants of Japanese White Rabbit Spermatozoa

2006 ◽  
Vol 52 (4) ◽  
pp. 511-516 ◽  
Author(s):  
Naomi KASHIWAZAKI ◽  
Yasushi OKUDA ◽  
Yasunari SEITA ◽  
Shin HISAMATSU ◽  
Shigenori SONOKI ◽  
...  
Keyword(s):  
Japanese White Rabbit ◽  
Rabbit Spermatozoa

scholarly journals Effects of diadenosine polyphosphates and seminal fluid vesicles on rabbit sperm cells

Reproduction ◽  
2003 ◽  
pp. 827-835 ◽  
Author(s):  
A Minelli ◽  
L Liguori ◽  
I Bellezza ◽  
T Renieri ◽  
C Castellini
Keyword(s):  
High Performance ◽  
Acrosome Reaction ◽  
Seminal Fluid ◽  
Membrane Vesicles ◽  
Fluorescein Isothiocyanate ◽  
Plasma Electron ◽  
Sperm Cells ◽  
Experimental Conditions ◽  
Diadenosine Polyphosphates ◽  
Rabbit Spermatozoa

Membrane vesicles were isolated from rabbit seminal plasma. Electron microscopy analyses showed the presence of numerous small, round vesicles with a diameter of about 70 nm. Determination of enzyme activities was carried out by high performance liquid chromatography and showed that the vesicles can degrade the diadenosine polyphosphates (ApnA), Ap3A and Ap4A and ATP and ADP, but not AMP. Studies of the degradation of diadenosine compounds by the vesicles present in seminal fluid showed an increasing production of AMP as the by-product and a time-dependent generation of dephosphorylated products consistent with the presence of ecto-ATP diphosphophosphatase (ecto-apyrase). In the presence of rabbit spermatozoa, AMP did not accumulate because 5'nucleotidase and adenosine deaminase, present at the surface of sperm cells, transformed AMP into adenosine and inosine. The effects of seminal fluid vesicles and diadenosine compounds on the acquisition of fertilizing capacity by rabbit spermatozoa were evaluated by Pisum sativum agglutinin fluorescein isothiocyanate conjugated staining. The results obtained with uncapacitated spermatozoa showed that the capacitating effector BSA could be substituted efficiently by the addition of diadenosine compounds and vesicles previously incubated for 2 h to the capacitative medium. Under these experimental conditions, the spontaneous acrosome reaction rate was not increased. Capacitated rabbit spermatozoa did not undergo acrosome reaction when l-alpha-lysophosphatidylcholine was substituted by diadenosine compounds previously incubated with vesicles. In conclusion, this study has shown that rabbit seminal fluid vesicles can degrade diadenosine compounds to AMP and that the addition of the vesicles and diadenosine compounds to uncapacitated rabbit spermatozoa favours the acquisition of the fertilizing capacity.

scholarly journals EMBRYONIC SURVIVAL SUBSEQUENT TO CULTURE OF RABBIT SPERMATOZOA AT 38  AND 40  C

Reproduction ◽  
1968 ◽  
Vol 15 (1) ◽  
pp. 87-92 ◽  
Author(s):  
P. J. BURFENING ◽  
L. C. ULBERG
Keyword(s):  
Embryonic Survival ◽  
Rabbit Spermatozoa

scholarly journals Chemotaxis of Capacitated Rabbit Spermatozoa to Follicular Fluid Revealed by a Novel Directionality-Based Assay1

2002 ◽  
Vol 67 (5) ◽  
pp. 1565-1571 ◽  
Author(s):  
Georgina Fabro ◽  
Roberto A. Rovasio ◽  
Silvia Civalero ◽  
Anat Frenkel ◽  
S. Roy Caplan ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Rabbit Spermatozoa

Development of Fertilizing Capacity of Rabbit Spermatozoa in the Uterus

Nature ◽  
1955 ◽  
Vol 175 (4467) ◽  
pp. 1036-1037 ◽  
Author(s):  
M. C. CHANG
Keyword(s):  
Rabbit Spermatozoa

scholarly journals The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro

2021 ◽  
Author(s):  
Marko Halo ◽  
Klaudia Bułka ◽  
Piotr A. Antos ◽  
Agnieszka Greń ◽  
Tomáš Slanina ◽  
...  
Keyword(s):  
Zno Nanoparticles ◽  
Spermatozoa Motility ◽  
Rabbit Spermatozoa

scholarly journals Impact of tilmicosin on the rabbit spermatozoa motility and viability

2016 ◽  
Vol 05 (Special issue 1) ◽  
pp. 53-56 ◽  
Author(s):  
Tomáš Slanina ◽  
Renáta Kováčová ◽  
Norbert Lukáč ◽  
Peter Massányi
Keyword(s):  
Spermatozoa Motility ◽  
Rabbit Spermatozoa
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