scholarly journals The N-terminal extension of Escherichia coli ribosomal protein L20 is important for ribosome assembly, but dispensable for translational feedback control

RNA ◽  
2005 ◽  
Vol 11 (5) ◽  
pp. 728-738 ◽  
Author(s):  
M. GUILLIER
Keyword(s):  
Escherichia Coli ◽  
Feedback Control ◽  
Ribosomal Protein ◽  
Ribosome Assembly

scholarly journals The GTPase BipA expressed at low temperature in Escherichia coli assists ribosome assembly and has chaperone-like activity

2018 ◽  
Vol 293 (47) ◽  
pp. 18404-18419 ◽  
Author(s):  
Eunsil Choi ◽  
Jihwan Hwang
Keyword(s):  
Escherichia Coli ◽  
Low Temperature ◽  
Ribosomal Protein ◽  
Ribosome Biogenesis ◽  
Cold Shock ◽  
Ribosomal Subunit ◽  
Protein Translation ◽  
Receptor Protein ◽  
Deletion Strain ◽  
Ribosome Assembly

BPI-inducible protein A (BipA) is a conserved ribosome-associated GTPase in bacteria that is structurally similar to other GTPases associated with protein translation, including IF2, EF-Tu, and EF-G. Its binding site on the ribosome appears to overlap those of these translational GTPases. Mutations in the bipA gene cause a variety of phenotypes, including cold and antibiotics sensitivities and decreased pathogenicity, implying that BipA may participate in diverse cellular processes by regulating translation. According to recent studies, a bipA-deletion strain of Escherichia coli displays a ribosome assembly defect at low temperature, suggesting that BipA might be involved in ribosome assembly. To further investigate BipA's role in ribosome biogenesis, here, we compared and analyzed the ribosomal protein compositions of MG1655 WT and bipA-deletion strains at 20 °C. Aberrant 50S ribosomal subunits (i.e. 44S particles) accumulated in the bipA-deletion strain at 20 °C, and the ribosomal protein L6 was absent in these 44S particles. Furthermore, bipA expression was significantly stimulated at 20 °C, suggesting that it encodes a cold shock–inducible GTPase. Moreover, the transcriptional regulator cAMP receptor protein (CRP) positively promoted bipA expression only at 20 °C. Importantly, GFP and α-glucosidase refolding assays revealed that BipA has chaperone activity. Our findings indicate that BipA is a cold shock–inducible GTPase that participates in 50S ribosomal subunit assembly by incorporating the L6 ribosomal protein into the 44S particle during the assembly.

scholarly journals Effects of Iodoamphenicol on Ribosome Assembly in Two Strains of Escherichia coli

Microbiology ◽  
1982 ◽  
Vol 128 (5) ◽  
pp. 997-1001
Author(s):  
P. D. Butler ◽  
P. F. G. Sims ◽  
D. G. Wild
Keyword(s):  
Escherichia Coli ◽  
Ribosome Assembly

Genetics of ribosomal protein methylation in Escherichia coli

1977 ◽  
Vol 154 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Jacques Lhoest ◽  
Charles Colson
Keyword(s):  
Escherichia Coli ◽  
Ribosomal Protein ◽  
Protein Methylation
Sign in / Sign up

Export Citation Format

Share Document