Honokiol, a chemopreventive agent against skin cancer, induces cell cycle arrest and apoptosis in human epidermoid A431 cells

2011 ◽  
Vol 236 (11) ◽  
pp. 1351-1359 ◽  
Author(s):  
Chandeshwari Chilampalli ◽  
Ruth Guillermo ◽  
Radhey S Kaushik ◽  
Alan Young ◽  
Gudiseva Chandrasekher ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Skin Cancer ◽  
Cell Cycle Arrest ◽  
Squamous Carcinoma ◽  
Ultraviolet B ◽  
Dependent Manner ◽  
A431 Cells ◽  
Squamous Carcinoma Cells ◽  
Cycle Arrest ◽  
M Phase

Honokiol is a plant lignan isolated from bark and seed cones of Magnolia officinalis. Recent studies from our laboratory indicated that honokiol pretreatment decreased ultraviolet B-induced skin cancer development in SKH-1 mice. The aim of the present investigation was to study the effects of honokiol on human epidermoid squamous carcinoma A431 cells and to elucidate possible mechanisms involved in preventing skin cancer. A431 cells were pretreated with different concentrations of honokiol for a specific time period and investigated for effects on apoptosis and cell cycle analysis. Treatment with honokiol significantly decreased cell viability and cell proliferation in a concentration- and time-dependent manner. Honokiol pretreatment at 50 μmol/L concentration induced G0/G1 cell cycle arrest significantly ( P < 0.05) and decreased the percentage of cells in the S and G2/M phase. Honokiol down-regulated the expression of cyclin D1, cyclin D2, Cdk2, Cdk4 and Cdk6 proteins and up-regulated the expression of Cdk's inhibitor proteins p21 and p27. Pretreatment of A431 cells with honokiol leads to induction of apoptosis and DNA fragmentation. These findings indicate that honokiol provides its effects in squamous carcinoma cells by inducing cell cycle arrest at G0/G1 phase and apoptosis.

Inhibition of ultraviolet B (UVB) induced apoptosis in A431 cells by mimosine is not dependent on cell cycle arrest

2002 ◽  
Vol 80 (7) ◽  
pp. 650-653
Author(s):  
D O Cliche ◽  
S Girouard ◽  
N Bissonnette ◽  
D J Hunting
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Rna Polymerase Ii ◽  
Ultraviolet B ◽  
Caspase Activation ◽  
A431 Cells ◽  
Cycle Arrest ◽  
Activation Assay ◽  
G1 Cell Cycle Arrest ◽  
Induced Apoptosis

Ultraviolet (UV) radiation is a strong apoptotic trigger in many cell types. We have previously reported that a plant amino acid, mimosine (beta-[N-(3-hydroxy-4-pyridone)]-alpha-aminopropionic acid), with a well-known reversible G1 cell cycle arrest activity can inhibit apoptosis induced by UV irradiation and RNA polymerase II blockage in human A431 cells. Here, apoptosis was measured with a fluorimetric caspase activation assay. Interestingly, the protective state was effective up to 24 h following removal of mimosine from the culture medium while cells were progressing in the cell cycle. Our results demonstrate that the protective effect of mimosine against UV-induced apoptosis can be dissociated from its G1 cell-cycle arrest activity.Key words: mimosine, apoptosis, cell cycle, A431 cells, caspase activation assay.

Mechanism of Ultraviolet B-Induced Cell Cycle Arrest in G2/M Phase in Immortalized Skin Keratinocytes with Defective p53

2000 ◽  
Vol 277 (1) ◽  
pp. 107-111 ◽  
Author(s):  
Mohammad Athar ◽  
Arianna L. Kim ◽  
Nihal Ahmad ◽  
Hasan Mukhtar ◽  
Jean Gautier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Ultraviolet B ◽  
Cycle Arrest ◽  
Skin Keratinocytes ◽  
M Phase

Chios Gum Mastic Induces Cell Cycle Arrest and Apoptosis in YD9 Human Oral Squamous Carcinoma Cells

2008 ◽  
Vol 21 (1) ◽  
pp. 55
Author(s):  
Jae Hyoun Park ◽  
Gyoo Cheon Kim ◽  
Hyun Ho Kwak ◽  
In Ryoung Kim ◽  
Seung Eun Lee ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Squamous Carcinoma ◽  
Carcinoma Cells ◽  
Squamous Carcinoma Cells ◽  
Cycle Arrest ◽  
Oral Squamous Carcinoma
Sign in / Sign up

Export Citation Format

Share Document