A simple, sensitive high-performance liquid chromatography -ultraviolet method for the quantification of concentration and steady-state pharmacokinetics of itraconazole and hydroxyitraconazole

2010 ◽  
Vol 47 (5) ◽  
pp. 432-439 ◽  
Author(s):  
M. Miura ◽  
N. Takahashi ◽  
M. Nara ◽  
N. Fujishima ◽  
H. Kagaya ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Steady State ◽  
High Performance

Neutral and DEAE dextrans as tracers for assessing lung microvascular barrier permeability and integrity

2002 ◽  
Vol 93 (1) ◽  
pp. 251-262 ◽  
Author(s):  
Jonathan R. Sanders ◽  
N. Adrienne Pou ◽  
Robert J. Roselli
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Steady State ◽  
High Performance ◽  
Capillary Permeability ◽  
Transport Mechanisms ◽  
Pulmonary Capillary ◽  
Before And After ◽  
Endogenous Proteins ◽  
Lung Lymph

Steady-state lymph-to-plasma concentration ratios (L/Ps) of neutral dextrans, cationic DEAE dextrans, and endogenous proteins were determined under normal and increased permeability conditions in six unanesthetized yearling sheep prepared with chronic lung lymph fistulas. Fluorescent dextrans with radii ranging from 1 to 30 nm were intravenously infused, and after 24 h, perilla ketone (PK) was given to alter permeability while the dextran infusion was maintained. Plasma and lymph samples were collected before and after PK administration and analyzed for dextran and protein concentrations after high-performance liquid chromatography size separation. Under both baseline and increased permeability conditions, DEAE dextrans had higher L/Ps than neutral dextrans of similar size but lower L/Ps than proteins of similar size. Comparison of L/Ps before and after PK revealed that the percentage change in permeability for neutral and DEAE dextrans was significantly larger than that for proteins. These results suggest that 1) the pulmonary microvascular barrier behaves as a net negative barrier, 2) some transport mechanisms for proteins and dextrans are different, and 3) neutral and cationic dextrans are more sensitive markers than proteins of the same size for assessing changes in pulmonary capillary permeability.

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