Monitoring Carotenoid and Chlorophyll Pigments in Virgin Olive Oil by Visible-Near Infrared Transmittance Spectroscopy. On-Line Application

Visible-near infrared transmittance spectroscopy was used to determine total levels of chlorophyll and carotenoid in virgin olive oil. Calibration models were developed in the laboratory using partial least squares regression. An initial smoothing followed by a first derivative treatment was the best signal correction. The validation set gave a correlation coefficient and standard error of prediction of 0.985 and 0.66 mg kg−1 for carotene totals and 0.993 and 0.96 mg kg−1 for chlorophyll totals. These partial least squares models were used to monitor on-line levels of these compounds during virgin olive oil processing in olive oil mills. The results indicate similarity between both visible-near infrared transmittance spectroscopy and reference laboratory methods.