Gas-Phase Chemistry of the Negative Ions of Fully-Protected Peptides by High-Resolution Electrospray Ionization Tandem Mass Spectrometry

2005 ◽  
Vol 11 (4) ◽  
pp. 403-408 ◽  
Author(s):  
Anna Napoli ◽  
Leonardo Di Donna ◽  
Giovanni Sindona ◽  
Elena Urso
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Tandem Mass Spectrometry ◽  
Mass Spectra ◽  
Negative Ions ◽  
Structural Data ◽  
Molecular Ions ◽  
Low Energy ◽  
Tandem Mass ◽  
Esi Ms

Fully-protected C-terminal free peptides can be conveniently analyzed by high-resolution electrospray tandem mass spectrometry (ESI-MS/MS) in a quadrupole quadrupole time-of-flight tandem hybrid mass spectrometer, operated in the negative (–) ionization mode. The unusual choice of negative ions in mass spectrometry applications to peptide analysis was needed to obtain exhaustive sequence and structural data. The low-energy collision-induced dissociation (CID) experiments provided, in fact, tandem mass spectra displaying highly diagnostic fragments with a good signal-to-noise ratio. The method is applied to segments of porcine calcitonin (Cal), Cal (10–16, 1), Cal (17–24, 2) and Cal (25–28, 3) whose [M–H]− deprotonated molecular ions provided low-energy CID mass spectra which allow the evaluation either of the primary structure of the peptide and of the location of the side-chain protective groups. ESI (+) MS can be conveniently used, in the high resolution mode, to achieve precise information on the elemental composition of the examined peptides.

Recent developments in the structural characterization of substituted meso-tetraarylporphyrins by electrospray tandem mass spectrometry

2009 ◽  
Vol 13 (04n05) ◽  
pp. 524-527 ◽  
Author(s):  
M. Rosário M. Domingues ◽  
Pedro Domingues ◽  
Maria G.P.M.S. Neves ◽  
Augusto C. Tomé ◽  
José A.S. Cavaleiro
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Structural Characterization ◽  
Mass Spectra ◽  
Molecular Ions ◽  
Tandem Mass ◽  
Aryl Ring ◽  
Esi Ms ◽  
Product Ions

meso-tetraarylporphyrins with different substituents linked either to an aryl ring or to a β-pyrrolic position were studied by Electrospray (ESI) Tandem Mass Spectrometry (MS/MS). The electrospray mass spectra (ESI-MS) of meso-tetraarylporphyrins show the molecular ions and no significant fragmentation is noted. Structural information can be obtained by inducing fragmentation of the ions generated under ESI conditions. The product ions formed and registered in the tandem mass spectra (ESI-MS/MS) are characteristic of the chemical structure of the groups linked to the porphyrin and depend on their location. Also, the same functional group linked either to the aryl ring or to the β-pyrrolic position yields different product ions that confirm their position. Taking into consideration the Portugal-Spain special issue of the Journal of Porphyrins and Phthalocyanines, in this highlight we analyze the work done at the University of Aveiro related to the use of mass spectrometry in the structural characterization of meso-tetraarylporphyrins with carbohydrate, nitro and amino acid groups.

Unrestrictive protein modification localization and quality control for open search of mass spectra

2018 ◽  
Author(s):  
Zhiwu An ◽  
Fuzhou Gong ◽  
Yan Fu
Keyword(s):  
Mass Spectrometry ◽  
Quality Control ◽  
Tandem Mass Spectrometry ◽  
Mass Spectra ◽  
Protein Modification ◽  
Software Tool ◽  
Tandem Mass ◽  
Simulation Data ◽  
Post Translational Modifications

We have developed PTMiner, a first software tool for automated, confident filtering, localization and annotation of protein post-translational modifications identified by open (mass-tolerant) search of large tandem mass spectrometry datasets. The performance of the software was validated on carefully designed simulation data. <br>

Direct sequencing of a disulfide-linked peptide with electrospray ionization tandem mass spectrometry

The Analyst ◽  
2015 ◽  
Vol 140 (8) ◽  
pp. 2623-2627 ◽  
Author(s):  
Gongyu Li ◽  
Jiying Pei ◽  
Yue Yin ◽  
Guangming Huang
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Corona Discharge ◽  
Electrospray Ionization ◽  
Disulfide Bonds ◽  
Direct Sequencing ◽  
Peptide Sequencing ◽  
Tandem Mass ◽  
Esi Ms ◽  
Ionization Tandem Mass Spectrometry

Enhanced corona discharge was employed for in-spray dissociation of disulfide bonds, facilitating disulfide-containing peptide sequencing with ESI-MS/MS.

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