High Performance Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry at Low Magnetic Field

2002 ◽  
Vol 8 (2) ◽  
pp. 169-176 ◽  
Author(s):  
Michael V. Gorshkov ◽  
Harold R. Udseth ◽  
Gordon A. Anderson ◽  
Richard D. Smith
Keyword(s):  
Magnetic Field ◽  
Mass Spectrometry ◽  
Fourier Transform ◽  
Electrospray Ionization ◽  
Cyclotron Resonance ◽  
High Performance ◽  
Ion Cyclotron Resonance ◽  
Ion Cyclotron ◽  
Low Magnetic Field ◽  
Ft Icr

A comprehensive characterization of a Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer utilizing a 1 tesla magnet with an electrospray ionization (ESI) source is presented. A resolution of up to 10,000,000 and a ppb-level mass accuracy has been achieved for atomic ions and light molecules produced with electron ionization. It has also been demonstrated that a resolution of up to 1,000,000 can be achieved for signals from biomolecule ions obtained with an ESI source, with the highest molecular weight for which the 13C isotopic pattern was completely resolved being that of ubiquitin (8565 Da). Several features of FT-ICR mass spectrometry at low magnetic field are discussed and approaches to overcome the low-field constraints are presented.

Molecular characterization of petroporphyrins in crude oil by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry

2001 ◽  
Vol 79 (5-6) ◽  
pp. 546-551 ◽  
Author(s):  
Ryan P Rodgers ◽  
Christopher L Hendrickson ◽  
Mark R Emmett ◽  
Alan G Marshall ◽  
Mark Greaney ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Crude Oil ◽  
Electrospray Ionization ◽  
Cyclotron Resonance ◽  
Spectrometric Analysis ◽  
Ionization Mass ◽  
Ion Cyclotron Resonance ◽  
Ion Cyclotron ◽  
Ft Icr

Petroporphyrin compositional analysis of a heavy crude oil has been realized by isolation and subsequent ESI-FT-ICR mass spectrometric analysis of the porphyrin-containing fractions. Vanadium octaethyl (V=O(II)OEP) and nickel octaethyl (Ni(II)OEP) porphyrin standards were analyzed to determine favorable electrospray ionization conditions and provide insight as to the molecular species present (e.g., adducts, multimers). Standard V=O(II)OEP and Ni(II)OEP solutions revealed the presence of both monomer and dimer species with a greater relative abundance of monomers. In contrast, mass spectral analysis of a porphyrin fraction from Cerro Negro crude oil was dominated by dimeric species. MS3 analysis identified a dioctylphthalate (DOP) contaminant, likely introduced during fractionation of the crude oil. DOP-porphyrin complexes and porphyrin-porphyrin dimers were then identified. Infrared multiphoton dissociation (IRMPD) of dimeric species produced the corresponding monomers with minimal fragmentation. The monomeric petroporphyrins were analyzed to reveal the metal (Ni(II) or V=O(II)), porphyrin type (e.g., etio vs. DPEP), and distribution of alkylation.Key words: petroporphyrin, porphyrin, petroleum, electrospray ionization, mass spectrometry, Fourier transform, ion cyclotron resonance, ICR, FT-ICR, FTMS.

Examples of Fourier Transform Ion Cyclotron Resonance Mass Spectrometry Developments: From Ion Physics to Remote Access Biochemical Mass Spectrometry

2005 ◽  
Vol 11 (5) ◽  
pp. 443-456 ◽  
Author(s):  
A. Römpp ◽  
I.M. Taban ◽  
R. Mihalca ◽  
M.C. Duursma ◽  
T.H. Mize ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Cyclotron Resonance ◽  
High Performance ◽  
Remote Access ◽  
Ion Cyclotron Resonance ◽  
Biological Macromolecules ◽  
Ion Cyclotron ◽  
Ft Icr Ms ◽  
Ft Icr

The application of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) for high-resolution biomolecular analysis has increased greatly after 30 years of innovation since its conception in 1974. FT-ICR-MS can now be used routinely for the analysis of complex organic mixtures such as biological or petrochemical samples. Many of these new possibilities have been the results of many different instrumental developments. This paper provides a mini review of selected instrumental developments that now allow these measurements. The development of soft ionization techniques such as electrospray ionization and matrix-assisted laser desorption and ionization was crucial for the analysis of biological macromolecules. Improved ion transport optics led to an increase in sensitivity. New ICR cell designs complement the capabilities of FT-ICR-MS by allowing a more thorough study of the mechanism and kinetics of ion reactions in the gas-phase. A selected example of electron capture dissociation (ECD) employs these developments to investigate the role of peptide conformation in ECD. Improved electronics and software allow faster and more flexible experiments. All these improvements led to an increase in speed and sensitivity that are necessary to couple FT-MS to fast separation techniques such as nano-high performance liquid chromatography. The modern FT-ICR-MS instruments can be incorporated in virtual organizations allowing remote access to unique infrastructure. This concept of remote experimentation opens new possibilities for scientific collaborations between expert scientists at different locations and allows the efficient use of this expensive instrumentation

The fingerprint of essential bio-oils by Fourier transform ion cyclotron resonance mass spectrometry

The Analyst ◽  
2020 ◽  
Vol 145 (9) ◽  
pp. 3414-3423
Author(s):  
Claudia X. Ramírez ◽  
Diana Catalina Palacio Lozano ◽  
Hugh E. Jones ◽  
Rafael Cabanzo Hernández ◽  
Mark P. Barrow ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Essential Oils ◽  
Electrospray Ionization ◽  
Cyclotron Resonance ◽  
Ion Cyclotron Resonance ◽  
Ion Cyclotron ◽  
Negative Electrospray Ionization ◽  
Ft Icr Ms ◽  
Ft Icr

Six essential oils were analyzed by Fourier transform ion cyclotron resonance mass spectrometry coupled to negative electrospray ionization (ESI(–)/FT-ICR MS).

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