Letter: The Use of 2-[(2E)-3-(4-Tert-Butylphenyl)-2-Methylprop-2-Enylidene]Malononitrile as a Matrix for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

2000 ◽  
Vol 6 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Lars Ulmer ◽  
Jochen Mattay ◽  
H. Gregorio Torres-Garcia ◽  
Heinrich Luftmann
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Ionization Mass ◽  
Radical Anions ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Evaporation Energy ◽  
Ion Production ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

2-[(2 E)-3-(4- tert-butylphenyl)-2-methylprop-2-enylidene]malononitrile (DCTB, dicyano. tert-butyl …) has proven to be an excellent nonprotic matrix for very labile compounds, especially for substituted fullerenes. This is mainly due to its extremely low onset of ion production. This matrix works mainly as an electron transfer agent, i.e. the ions produced are radical anions or cations, respectively. It works equally well to transfer evaporation energy to charged oligomers and with proton labile compounds (not shown here).

scholarly journals Synthesis and Matrix Properties of α-Cyano-5-phenyl-2,4-pentadienic Acid (CPPA) for Intact Proteins Analysis by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Molecules ◽  
2020 ◽  
Vol 25 (24) ◽  
pp. 6054
Author(s):  
Antonio Monopoli ◽  
Angelo Nacci ◽  
Tommaso R. I. Cataldi ◽  
Cosima D. Calvano
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Protein Analysis ◽  
Ionization Mass Spectrometry ◽  
Intact Protein ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Desorption Ionization ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

The effectiveness of a synthesized matrix, α-cyano-5-phenyl-2,4-pentadienic acid (CPPA), for protein analysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in complex samples such as foodstuff and bacterial extracts, is demonstrated. Ultraviolet (UV) absorption along with laser desorption/ionization mass spectrometry (LDI-MS) experiments were systematically conducted in positive ion mode under standard Nd:YLF laser excitation with the aim of characterizing the matrix in terms of wavelength absorption and proton affinity. Besides, the results for standard proteins revealed that CPPA significantly enhanced the protein signals, reduced the spot-to-spot variability and increased the spot homogeneity. The CPPA matrix was successful employed to investigate intact microorganisms, milk and seed extracts for protein profiling. Compared to conventional matrices such as sinapinic acid (SA), α-cyano-4-hydroxycinnamic acid (CHCA) and 4-chloro-α-cyanocinnamic acid (CClCA), CPPA exhibited better signal-to-noise (S/N) ratios and a uniform response for most examined proteins occurring in milk, hazelnut and in intact bacterial cells of E. coli. These findings not only provide a reactive proton transfer MALDI matrix with excellent reproducibility and sensitivity, but also contribute to extending the battery of useful matrices for intact protein analysis.

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