Analysis of ruthenium carbonyl–porphyrin complexes: a comparison of matrix-assisted laser desorption/ionisation time-of-flight, fast-atom bombardment and field desorption mass spectrometry

1997 ◽  
Vol 3 (1) ◽  
pp. 427 ◽  
Author(s):  
Matthias Frauenkron ◽  
Albrecht Berkessel ◽  
Jürgen Gross
Keyword(s):  
Mass Spectrometry ◽  
Fast Atom Bombardment ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Field Desorption ◽  
Desorption Mass Spectrometry ◽  
Ruthenium Carbonyl ◽  
Field Desorption Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Characterization of Potential Protein Biomarkers for Major Depressive Disorder Using Matrix-Assisted Laser Desorption Ionization/Time-of-Flight Mass Spectrometry

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4457
Author(s):  
Chieh-Hsin Lin ◽  
Hung Su ◽  
Chung-Chieh Hung ◽  
Hsien-Yuan Lane ◽  
Jentaie Shiea
Keyword(s):  
Mass Spectrometry ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Healthy Controls ◽  
Laser Desorption Ionization ◽  
Major Depressive ◽  
Ionization Time ◽  
Maldi Tof ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

Matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry is a sensitive analytical tool for characterizing various biomolecules in biofluids. In this study, MALDI-TOF was used to characterize potential plasma biomarkers for distinguishing patients with major depressive disorder (MDD) from patients with schizophrenia and healthy controls. To avoid interference from albumin—the predominant protein in plasma—the plasma samples were pretreated using acid hydrolysis. The results obtained by MALDI-TOF were also validated by electrospray ionization-quadrupole time-of-flight (ESI-QTOF) mass spectrometry. The analytical results were further treated with principal component analysis (PCA), hierarchical clustering analysis (HCA), and receiver operating characteristic (ROC) curve analysis. The statistical analyses showed that MDD patients could be distinguished from schizophrenia patients and healthy controls by the lack of apolipoprotein C1 (Apo C1), which, in fact, was detected in healthy controls and schizophrenia patients. This protein is suggested to be a potential plasma biomarker for distinguishing MDD patients from healthy controls and schizophrenia patients. Since sample preparation for MALDI-TOF is very simple, high-throughput plasma apolipoprotein analysis for clinical purposes is feasible.

Analysis of artificial proteins by matrix-assisted laser desorption mass spectrometry

1992 ◽  
Vol 114 (19) ◽  
pp. 7584-7585 ◽  
Author(s):  
Ronald C. Beavis ◽  
Brian T. Chait ◽  
Howard S. Creel ◽  
Maurille J. Fournier ◽  
Thomas L. Mason ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Desorption ◽  
Laser Desorption Mass Spectrometry ◽  
Desorption Mass Spectrometry ◽  
Artificial Proteins ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

scholarly journals Characterization of Cryptosporidium parvum by Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2000 ◽  
Vol 66 (11) ◽  
pp. 4720-4724 ◽  
Author(s):  
Matthew L. Magnuson ◽  
James H. Owens ◽  
Catherine A. Kelty
Keyword(s):  
Mass Spectrometry ◽  
Cryptosporidium Parvum ◽  
Time Of Flight ◽  
Laser Desorption ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Desorption Ionization ◽  
Flight Mass Spectrometry ◽  
Matrix Assisted Laser ◽  
Matrix Assisted Laser Desorption

ABSTRACT Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) was used to investigate whole and freeze-thawed Cryptosporidium parvum oocysts. Whole oocysts revealed some mass spectral features. Reproducible patterns of spectral markers and increased sensitivity were obtained after the oocysts were lysed with a freeze-thaw procedure. Spectral-marker patterns forC. parvum were distinguishable from those obtained forCryptosporidium muris. One spectral marker appears specific for the genus, while others appear specific at the species level. Three different C. parvum lots were investigated, and similar spectral markers were observed in each. Disinfection of the oocysts reduced and/or eliminated the patterns of spectral markers.

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