Mass Spectrometry as an Efficient Tool for the Characterization of Amyloid β Peptide 25–35 Self-Assembly Species in Aggregation and Inhibition Studies

2013 ◽  
Vol 19 (6) ◽  
pp. 483-490 ◽  
Author(s):  
J. Fiori ◽  
M. Naldi ◽  
V. Andrisano
Keyword(s):  
Mass Spectrometry ◽  
Self Assembly ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Efficient Tool ◽  
Inhibition Studies

scholarly journals Seeing the invisible by paramagnetic and diamagnetic NMR

2013 ◽  
Vol 41 (6) ◽  
pp. 1343-1354 ◽  
Author(s):  
G. Marius Clore
Keyword(s):  
Self Assembly ◽  
Large Scale ◽  
Amyloid Β ◽  
Cavity Surface ◽  
Internal Cavity ◽  
Amyloid Β Peptide ◽  
Line Broadening ◽  
Transient Nature ◽  
Chaperonin Groel

Sparsely populated transient states of proteins and their complexes play an important role in many biological processes including protein–protein and protein–DNA recognition, allostery, conformational selection, induced fit and self-assembly. These states are difficult to study as their low population and transient nature makes them effectively invisible to conventional structural and biophysical techniques. In the present article, I summarize recent NMR developments in our laboratory, including the use of paramagnetic relaxation enhancement, lifetime line broadening and dark-state exchange saturation transfer spectroscopy, that have permitted such sparsely populated states to be detected, characterized and, in some instances, visualized. I illustrate the application of these methods to the elucidation of mechanisms whereby transcription factors locate their specific target sites within an overwhelming sea of non-specific DNA, to the characterization of encounter complexes in protein–protein recognition, to large-scale interdomain motions involved in ligand binding, and to the interaction of monomeric amyloid β-peptide with the surface of amyloid protofibrils and the internal cavity surface of the chaperonin GroEL.

Enhanced self-assembly of the 7–12 sequence of amyloid-β peptide by tyrosine bromination

2020 ◽  
Vol 32 (4) ◽  
pp. 247-255 ◽  
Author(s):  
Daniele Maiolo ◽  
Andrea Pizzi ◽  
Alessandro Gori ◽  
Greta Bergamaschi ◽  
Claudia Pigliacelli ◽  
...  
Keyword(s):  
Self Assembly ◽  
Amyloid Β ◽  
Amyloid Β Peptide

Quenched hydrogen/deuterium exchange NMR characterization of amyloid-β peptide aggregates formed in the presence of Cu2+or Zn2+

FEBS Journal ◽  
2009 ◽  
Vol 276 (15) ◽  
pp. 4051-4060 ◽  
Author(s):  
Anders Olofsson ◽  
Malin Lindhagen-Persson ◽  
Monika Vestling ◽  
A. Elisabeth Sauer-Eriksson ◽  
Anders Öhman
Keyword(s):  
Amyloid Β ◽  
Deuterium Exchange ◽  
Amyloid Β Peptide ◽  
Hydrogen Deuterium Exchange ◽  
Nmr Characterization ◽  
Hydrogen Deuterium

Chemical Cross-Linking/Mass Spectrometry Maps the Amyloid β Peptide Binding Region on Both Apolipoprotein E Domains

2015 ◽  
Vol 10 (4) ◽  
pp. 1010-1016 ◽  
Author(s):  
Stéphanie Deroo ◽  
Florian Stengel ◽  
Azadeh Mohammadi ◽  
Nicolas Henry ◽  
Ellen Hubin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Apolipoprotein E ◽  
Peptide Binding ◽  
Amyloid Β ◽  
Cross Linking ◽  
Amyloid Β Peptide ◽  
Binding Region ◽  
Peptide Binding Region ◽  
Chemical Cross Linking

Characterization of Amyloid β Peptides in Cerebrospinal Fluid by an Automated Immunoprecipitation Procedure Followed by Mass Spectrometry

2007 ◽  
Vol 6 (11) ◽  
pp. 4433-4439 ◽  
Author(s):  
Erik Portelius ◽  
Ai Jun Tran ◽  
Ulf Andreasson ◽  
Rita Persson ◽  
Gunnar Brinkmalm ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cerebrospinal Fluid ◽  
Amyloid Β

scholarly journals Characterization of the Binding of Amyloid-β Peptide to Cell Culture-Derived Native Apolipoprotein E2, E3, and E4 Isoforms and to Isoforms from Human Plasma

2002 ◽  
Vol 68 (2) ◽  
pp. 721-725 ◽  
Author(s):  
Dun-Sheng Yang ◽  
Jonathan D. Smith ◽  
Zhongmin Zhou ◽  
Samuel E. Gandy ◽  
Ralph N. Martins
Keyword(s):  
Cell Culture ◽  
Human Plasma ◽  
Amyloid Β ◽  
Amyloid Β Peptide

Identification and Nanomechanical Characterization of the HIV Tat‐Amyloid β Peptide Multifibrillar Structures

2020 ◽  
Vol 26 (43) ◽  
pp. 9449-9453
Author(s):  
Qiying Feng ◽  
Yue Hong ◽  
Naga Pradeep Nidamanuri ◽  
Chuanxu Yang ◽  
Qiang Li ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Hiv Tat
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