scholarly journals Characteristics of ATP-Induced Current Through P2X7 Receptor in NG108-15 Cells: Unique Antagonist Sensitivity and Lack of Pore Formation

2002 ◽  
Vol 88 (4) ◽  
pp. 428-435 ◽  
Author(s):  
Tomokazu Watano ◽  
Isao Matsuoka ◽  
Junko Kimura
Keyword(s):  
P2x7 Receptor ◽  
Pore Formation ◽  
Induced Current

scholarly journals Inhibitory Effects of Metals on ATP-Induced Current Through P2X7 Receptor in NG108-15 Cells

2002 ◽  
Vol 89 (3) ◽  
pp. 296-301 ◽  
Author(s):  
Tomokazu Watano ◽  
Isao Matsuoka ◽  
Junko Kimura
Keyword(s):  
P2x7 Receptor ◽  
Induced Current ◽  
Inhibitory Effects

scholarly journals Epidermal Growth Factor Facilitates Epinephrine Inhibition of P2X7-Receptor-Mediated Pore Formation and Apoptosis: A Novel Signaling Network

Endocrinology ◽  
2005 ◽  
Vol 146 (1) ◽  
pp. 164-174 ◽  
Author(s):  
Liqin Wang ◽  
Ying-Hong Feng ◽  
George I. Gorodeski
Keyword(s):  
Plasma Membrane ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
P2x7 Receptor ◽  
Pore Formation ◽  
Cell Number ◽  
Epidermal Growth ◽  
Phosphoinositide 3 Kinase ◽  
Dependent Mechanism

Epidermal growth factor (EGF), epinephrine, and the P2X7 receptor system regulate growth of human uterine cervical epithelial cells, but little is known about how these systems intercommunicate in exerting their actions. The objective of this study was to understand the mechanisms of EGF and epinephrine regulation of growth of cervical cells. Treatment of cultured CaSki cells with 0.2 nm EGF increased cell number via a PD98059-sensitive pathway. Treatment with 2 nm epinephrine increased cell number, and the effect was facilitated by cotreatment with EGF. Whereas the effect of EGF alone involved up-regulation of [3H]-thymidine incorporation and an increase in cell proliferation, the effect of epinephrine was mediated by inhibition of apoptosis. Epinephrine inhibited apoptosis induced by the P2X7 receptor ligand 2′,3′-0-(4-benzoylbenzoyl)-ATP, by attenuation of P2X7 receptor plasma membrane pore formation. Cotreatment with EGF facilitated epinephrine effect via a phosphoinositide 3-kinase-dependent mechanism. CaSki cells express the β2-adrenoceptor, and the epinephrine antiapoptotic effect could be mimicked by β2-adrenoceptor agonists and by activators of adenylyl cyclase. Likewise, the effect could be blocked by β2-adrenoceptor blockers and by the inhibitor of protein kinase-A H-89. Western immunoblot analysis revealed that epinephrine decreased the levels of the glycosylated 85-kDa form of the P2X7 receptor and increased receptor degradation, and that EGF potentiated these effects of epinephrine. EGF did not affect cellular levels of the β2-adrenoceptor. In contrast, EGF, acting via the EGF receptor, augmented β2-adrenoceptor recycling, and it inhibited β2-adrenoceptor internalization via a phosphoinositide 3-kinase-dependent mechanism. We conclude that, in cervical epithelial cells, EGF has a dual role: as mitogen, acting via the MAPK/MAPK kinase pathway, and as an antiapoptotic factor by facilitating epinephrine effect and resulting in greater expression of β2-adrenoceptors in the plasma membrane. These findings underscore a novel signaling network of communication between the receptor tyrosine kinases, the G protein-coupled receptors, and the purinergic P2X7 receptor.

scholarly journals Non-nucleotide Agonists Triggering P2X7 Receptor Activation and Pore Formation

2018 ◽  
Vol 9 ◽  
Author(s):  
Francesco Di Virgilio ◽  
Anna L. Giuliani ◽  
Valentina Vultaggio-Poma ◽  
Simonetta Falzoni ◽  
Alba C. Sarti
Keyword(s):  
P2x7 Receptor ◽  
Pore Formation ◽  
Receptor Activation ◽  
P2x7 Receptor Activation

scholarly journals P2X7 Receptor Cell Surface Expression and Cytolytic Pore Formation Are Regulated by a Distal C-terminal Region

2002 ◽  
Vol 278 (10) ◽  
pp. 8853-8860 ◽  
Author(s):  
Megan L. Smart ◽  
Ben Gu ◽  
Rekha G. Panchal ◽  
James Wiley ◽  
Brett Cromer ◽  
...  
Keyword(s):  
Cell Surface ◽  
P2x7 Receptor ◽  
Pore Formation ◽  
Receptor Cell ◽  
Surface Expression ◽  
Terminal Region ◽  
Cell Surface Expression

scholarly journals Genetically determined P2X7 receptor pore formation regulates variability in chronic pain sensitivity

2012 ◽  
Vol 18 (4) ◽  
pp. 595-599 ◽  
Author(s):  
Robert E Sorge ◽  
Tuan Trang ◽  
Ruslan Dorfman ◽  
Shad B Smith ◽  
Simon Beggs ◽  
...  
Keyword(s):  
Chronic Pain ◽  
P2x7 Receptor ◽  
Pore Formation ◽  
Pain Sensitivity ◽  
Genetically Determined
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