scholarly journals Bifemelane Protects Cultured Cortical Neurons Against N-Methyl-D-aspartate Receptor-Mediated Glutamate Cytotoxicity

1998 ◽  
Vol 76 (3) ◽  
pp. 313-316 ◽  
Author(s):  
Akinori Akaike ◽  
Takehiko Maeda ◽  
Toshiaki Kume ◽  
Satoshi Kaneko
Keyword(s):  
Cortical Neurons ◽  
Aspartate Receptor ◽  
Cultured Cortical Neurons

Nicotine-induced protection of cultured cortical neurons againstN-methyl-D-aspartate receptor-mediated glutamate cytotoxicity

1994 ◽  
Vol 644 (2) ◽  
pp. 181-187 ◽  
Author(s):  
Akinori Akaike ◽  
Yutaka Tamura ◽  
Takeharu Yokota ◽  
Shun Shimohama ◽  
Jun Kimura
Keyword(s):  
Cortical Neurons ◽  
Aspartate Receptor ◽  
Cultured Cortical Neurons

Prostaglandin E2 protects cultured cortical neurons against N-methyl-d-aspartate receptor-mediated glutamate cytotoxicity

1994 ◽  
Vol 663 (2) ◽  
pp. 237-243 ◽  
Author(s):  
Akinori Akaike ◽  
Shuji Kaneko ◽  
Yutaka Tamura ◽  
Noritaka Nakata ◽  
Hirohito Shiomi ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Cortical Neurons ◽  
Aspartate Receptor ◽  
Cultured Cortical Neurons

scholarly journals The Bacterial Enzyme Cas13 Interferes with Neurite Outgrowth from Cultured Cortical Neurons

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 262
Author(s):  
Qin-Wei Wu ◽  
Josef P. Kapfhammer
Keyword(s):  
Rna Editing ◽  
Cortical Neurons ◽  
High Efficiency ◽  
Primary Cultures ◽  
Cultured Neurons ◽  
Therapeutic Tool ◽  
Bacterial Enzyme ◽  
Rna Targeting ◽  
Cultured Cortical Neurons ◽  
New Generation

The CRISPR-Cas13 system based on a bacterial enzyme has been explored as a powerful new method for RNA manipulation. Due to the high efficiency and specificity of RNA editing/interference achieved by this system, it is currently being developed as a new therapeutic tool for the treatment of neurological and other diseases. However, the safety of this new generation of RNA therapies is still unclear. In this study, we constructed a vector expressing CRISPR-Cas13 under a constitutive neuron-specific promoter. CRISPR-Cas13 from Leptotrichia wadei was expressed in primary cultures of mouse cortical neurons. We found that the presence of CRISPR-Cas13 impedes the development of cultured neurons. These results show a neurotoxic action of Cas13 and call for more studies to test for and possibly mitigate the toxic effects of Cas13 enzymes in order to improve CRISPR-Cas13-based tools for RNA targeting.

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