scholarly journals Effects of Xanthine Derivatives on Phosphatidylcholine Secretion in Primary Culture of Rat Type II Pneumocytes

1995 ◽  
Vol 67 (2) ◽  
pp. 165-168 ◽  
Author(s):  
Manabu Okumura ◽  
Michio Tsuruoka ◽  
Yoichiro Isohama ◽  
Hirofumi Kai ◽  
Kazuo Takahama ◽  
...  
Keyword(s):  
Primary Culture ◽  
Type Ii ◽  
Xanthine Derivatives ◽  
Type Ii Pneumocytes ◽  
Phosphatidylcholine Secretion

scholarly journals Effects of Xanthine Derivatives on Phosphatidylcholine Secretion in Rat Type II Pneumocytes in the Presence of Activated Eosinophils

1997 ◽  
Vol 75 (4) ◽  
pp. 425-432 ◽  
Author(s):  
Manabu Okumura ◽  
Hirofumi Kai ◽  
Shinya Shinozawa ◽  
Yoichiro Isohama ◽  
Kazuo Takahama ◽  
...  
Keyword(s):  
Type Ii ◽  
Xanthine Derivatives ◽  
Type Ii Pneumocytes ◽  
Phosphatidylcholine Secretion

The effect of stone-wool on rat lungs and on the primary culture of rat alveolar macrophages and type II pneumocytes

2005 ◽  
Vol 26 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Erzsébet Tátrai ◽  
Márta Brozik ◽  
Ágnes Drahos ◽  
Zuzana Kováčiková ◽  
Éva Six ◽  
...  
Keyword(s):  
Primary Culture ◽  
Alveolar Macrophages ◽  
Type Ii ◽  
Rat Lungs ◽  
Type Ii Pneumocytes ◽  
Stone Wool

Pyridine Derivatives Stimulate Phosphatidylcholine Secretion in Primary Cultures of Rat Type II Pneumocytes

1996 ◽  
Vol 48 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Hirofumi Kai ◽  
Koichiro Murahara ◽  
Yoichiro Isohama ◽  
Kazuo Takahama ◽  
Yoshiaki Oda ◽  
...  
Keyword(s):  
Primary Cultures ◽  
Type Ii ◽  
Pyridine Derivatives ◽  
Type Ii Pneumocytes ◽  
Phosphatidylcholine Secretion

Biochemical and metabolic properties of bovine type II pneumocytes in primary culture

Lung ◽  
1989 ◽  
Vol 167 (1) ◽  
pp. 343-350 ◽  
Author(s):  
H. G. Augustin-Voss ◽  
H. A. Schoon ◽  
A. Gerull ◽  
S. Ueberschär
Keyword(s):  
Primary Culture ◽  
Type Ii ◽  
Type Ii Pneumocytes ◽  
Metabolic Properties ◽  
Bovine Type

Adrenomedullin increases phosphatidylcholine secretion in rat type II pneumocytes

2000 ◽  
Vol 403 (3) ◽  
pp. 189-194 ◽  
Author(s):  
Manabu Okumura ◽  
Hirofumi Kai ◽  
Kazuhiko Arimori ◽  
Tomomi Iwakiri ◽  
Muneaki Hidaka ◽  
...  
Keyword(s):  
Type Ii ◽  
Type Ii Pneumocytes ◽  
Phosphatidylcholine Secretion

Density-independent isolation of type II pneumocytes after partial pneumonectomy

1989 ◽  
Vol 256 (3) ◽  
pp. C515-C521 ◽  
Author(s):  
B. D. Uhal ◽  
G. D. Hess ◽  
D. E. Rannels
Keyword(s):  
Primary Culture ◽  
Compensatory Growth ◽  
Thymidine Incorporation ◽  
Maximal Velocity ◽  
Type Ii ◽  
Percoll Gradient ◽  
Type Ii Pneumocytes ◽  
The Right ◽  
Immunoglobin G ◽  
Exogenous Spermidine

Type II pneumocytes were isolated by immunoglobin G (IgG) panning from the lungs of normal rats and the right lung of rats (180-200 g) subjected to left pneumonectomy. Cells were studied at 7 (pnx-7) and 15 (pnx-15) days postoperative, times during and after, respectively, rapid compensatory growth of the right lung. After 24 h of primary culture, pnx-7 cells contained 32% more protein per DNA, and incorporated thymidine at a rate 224% greater than cells isolated from control rats. Both the protein-to-DNA ratio and thymidine incorporation returned to control values in pnx-15 cells. Uptake of exogenous spermidine also was increased by 50% in pnx-7 cells at 24 h of primary culture and returned to control values in pnx-15 cells. Increased spermidine uptake was due to an increase in the maximal velocity (Vmax) of transport from 30.3 (control) to 45.5 pmol.micrograms DNA-1.h-1 (pnx-7), with no change in the apparent Km of 1.32 microM. No change was observed in the relative rates of phospholipid or neutral lipid biosynthesis. The increases in thymidine incorporation and spermidine uptake were significantly greater than those previously observed [Am. J. Physiol. 254 (Cell Physiol. 23): C684-C690, 1988] in pnx-7 cells isolated by Percoll gradient sedimentation. These results suggest that pnx-7 lungs contain distinct subpopulations of type II pneumocytes, the recovery of which is dependent on the cell isolation protocol employed.

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