scholarly journals Identification and Characterization of the α2d-Adrenoceptor Subtype in Single Cells Prepared from Guinea Pig Tracheal Smooth Muscles

1992 ◽  
Vol 60 (4) ◽  
pp. 393-395 ◽  
Author(s):  
Mitsutoshi Satoh ◽  
Issei Takayanagi
Keyword(s):  
Guinea Pig ◽  
Single Cells ◽  
Smooth Muscles ◽  
Identification And Characterization

Relationship between potency of L-isoprenaline and β-adrenoceptor density estimated in single cells from tracheal smooth muscles of guinea pigs of different ages

1992 ◽  
Vol 70 (4) ◽  
pp. 458-461 ◽  
Author(s):  
Issei Takayanagi ◽  
Mitsutoshi Satoh ◽  
Noriko Kokubu ◽  
Teruko Kato
Keyword(s):  
Guinea Pig ◽  
Guinea Pigs ◽  
Dissociation Constants ◽  
Specific Binding ◽  
Single Cells ◽  
Regression Line ◽  
Smooth Muscles ◽  
Receptor Density ◽  
Age Related ◽  
Β Receptor

An age-related change in potency of L-isoprenaline in the presence of ascorbic acid, desmethylimipramine, corticosterone, pargyline, and phentolamine was obtained in tracheal strips from guinea pigs of differing ages between 6 and 40 weeks. The potency in the strips from 100-week-old guinea pigs did not significantly differ from that in strips from 40-week-old animals. Single cells were prepared from the tracheal muscles of 6-, 10-, 40-, and 100-week-old guinea pigs. The specific binding of [3H]dihydroalprenolol to the single cells was saturable. The dissociation constants of [3H]dihydroalprenolol were in good agreement with those of the membrane fractions from the guinea-pig tracheal muscles, and did not change with age. An excellent relationship between the potency of L-isoprenaline and the maximum binding of [3H]dihydroalprenolol estimated in the preparations from 6- to 40-week-old guinea pigs was found, suggesting that the increase in the potency of L-isoprenaline is due to the increase in the maximum binding or receptor density. The value in the preparations from 100-week-old guinea pigs deviated significantly from the regression line. This suggests the possibility that the decrease in potency in the strips from 100-week-old animals is due to a change in post β-receptor processes in responsiveness.Key words: guinea-pig trachea, single cells, β-receptor density, ageing, dissociation constant.

Identification and characterization of an early estrogen-regulated RNA in cultured guinea-pig endometrial cells

1993 ◽  
Vol 90 (2) ◽  
pp. R17-R21 ◽  
Author(s):  
I. Pellerin ◽  
C. Vuillermoz ◽  
M. Jouvenot ◽  
C. Ordener ◽  
M. Royez ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Endometrial Cells ◽  
Identification And Characterization

Identification and characterization of the principal proteins of the fat-globule membrane from guinea-pig milk

1985 ◽  
Vol 151 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Virginia G. JOHNSON ◽  
Dale E. GREENWALT ◽  
Hans W. HEID ◽  
Ian H. MATHER ◽  
Patricia J. MADARA
Keyword(s):  
Guinea Pig ◽  
Fat Globule ◽  
Identification And Characterization
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