scholarly journals Mechanism f inhibitory action of tranilast on the release of slow reacting substance of anaphylaxis (SRS-A) in vitro: Effect of tranilast on the release of arachidonic acid and its metabolites.

1988 ◽  
Vol 46 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Hidetada KOMATSU ◽  
Masami KOJIMA ◽  
Naoyuki TSUTSUMI ◽  
Shuichiro HAMANO ◽  
Hiroshi KUSAMA ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Inhibitory Action ◽  
Vitro Effect

scholarly journals The In Vitro Effect of Steroid Hormones, Arachidonic Acid, and Kinases Inhibitors on Aquaporin 1, 2, 5, and 7 Gene Expression in the Porcine Uterine Luminal Epithelial Cells during the Estrous Cycle

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 832
Author(s):  
Damian Tanski ◽  
Agnieszka Skowronska ◽  
Malgorzata Tanska ◽  
Ewa Lepiarczyk ◽  
Mariusz T. Skowronski
Keyword(s):  
Arachidonic Acid ◽  
Epithelial Cells ◽  
Steroid Hormones ◽  
Estrous Cycle ◽  
Kinase Inhibitor ◽  
Mapk Signaling ◽  
Mapk Kinase ◽  
Vitro Effect ◽  
Luminal Epithelial Cells

Aquaporins (AQPs) are integral membrane proteins, which play an important role in water homeostasis in the uterus. According to the literature, the expression of aquaporins in reproductive structures depends on the local hormonal milieu. The current study investigated the effect of selected PKA kinase inhibitor H89 and MAPK kinase inhibitor PD98059, on the expression of AQP1, 2, 5, and 7, and steroid hormones (E2), progesterone (P4), and arachidonic acid (AA) in the porcine endometrium on days 18–20 and 2–4 of the estrous cycle (the follicular phase where estrogen and follicle-stimulating hormone (FSH) are secreted increasingly in preparation for estrus and the luteal phase where the ovarian follicles begin the process of luteinization with the formation of the corpus luteum and progesterone secretion, respectively). The luminal epithelial cells were incubated in vitro in the presence of the aforementioned factors. The expression of mRNA was determined by the quantitative real-time PCR technique. In general, in Experiment 1, steroid hormones significantly increased expression of AQP1, 2, and 5 while arachidonic acid increased expression of AQP2 and AQP7. On the other hand, MAPK kinase inhibitor significantly decreased the expression of AQP1 and 5. In Experiment 2, E2, P4, or AA combined with kinase inhibitors differentially affected on AQPs expression. E2 in combination with PKA inhibitor significantly decreased expression of AQP1 but E2 or P4 combined with this inhibitor increased the expression of AQP5 and 7. On the contrary, E2 with PD98059 significantly increased AQP5 and AQP7 expression. Progesterone in combination with MAPK kinase inhibitor significantly downregulated the expression of AQP5 and upregulated AQP7. Arachidonic acid mixed with H89 or PD98059 caused a decrease in the expression of AQP5 and an increase of AQP7. The obtained results indicate that estradiol, progesterone, and arachidonic acid through PKA and MAPK signaling pathways regulate the expression of AQP1 and AQP5 in the porcine luminal epithelial cells in the periovulatory period.

STUDIES ON THE EFFECT OF SOME AROMATIC AMIDINES AND OTHER CHEMICAL COMPOUNDS ON THE GROWTH OF INFLUENZA VIRUS IN THE EMBRYONATED EGG

1949 ◽  
Vol 27e (3) ◽  
pp. 177-185 ◽  
Author(s):  
Laurella McClelland ◽  
C. E. van Rooyen
Keyword(s):  
Influenza Virus ◽  
Inhibitory Action ◽  
Chemical Compounds ◽  
Fundamental Study ◽  
Vitro Effect

The effect of chemicals and biological substances on the growth of influenza virus in the embryonated egg is of interest as a fundamental study and as a means of screening substances for their possible chemotherapeutic activities. This work was done to investigate the activity of some heretofore unreported substances. Most chemicals proved to have no effect on the growth of influenza virus in the developing egg, but one group, the aromatic amidines, and hexamidine in particular, was found to possess some inhibitory action. It was an in vivo rather than an in vitro effect; it was dependent on the route of inoculation and the amount of drug administered; and the activity was exhibited when the introduction of the drug was delayed for as long as 16 hr. after inoculation of the eggs with influenza virus. Preliminary tests have failed to demonstrate any protection of mice infected with influenza and treated with hexamidine.

In Vitro Effect Of Ticlopidine On Arachidonic Acid Metabolism In Platelet Phospholipids

1981 ◽  
Author(s):  
H Chap ◽  
M F Simon ◽  
L Douste-Blazy
Keyword(s):  
Arachidonic Acid ◽  
Acid Metabolism ◽  
Arachidonic Acid Metabolism ◽  
Maximum Effect ◽  
Prostaglandin D2 ◽  
Intact Cells ◽  
Vitro Effect ◽  
Slight Inhibition ◽  
Acylation Reactions

The effects of ticlopidine (Ti) on arachidonic acid (AA) metabolism in platelet phospholipids have been studied in vitro by following AA release from (14C) AA-prelabeled platelets or by measuring (14C)-AA incorporation into platelet phospholipids.In the presence of prelabeled platelets, Ti induced a release of AA essentially from phosphatidylcholine (PC) only at concentrations (10-3 M) where the drug became lytic. Incubation of cells previously lysed by sonication led to a deacylation of PC, part of the released AA being reincorporated into phosphatidylethanolamine (PE) . Under these conditions, Ti effect on PC disappeared and only a slight inhibition of AA incorporation into PE was observed.On the other hand, upon incubation of non-labeled platelets with (14C)-AA, Ti impaired the incorporation of AA into all platelet phospholipids, half maximum effect being observed under non-lytic conditions at 10-5-5.10-5 M.It is postulated that Ti inhibits the acylation reactions responsible for AA entry into glycerophospholipids. This effect might promote the release of trace amounts of AA in intact cells, which could explain the accumulation of stable, anti-aggregating prostaglandin D2 (M. Lagarde et al. Prostagl. Med. 1979, 2, 433).

In vitro effect of an aqueous extract of Artocarpus lakoocha on the intestinal parasites in cattle

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Saowakon ◽  
P Chaichanasak ◽  
C Wanichanon ◽  
V Reutrakul ◽  
P Sobhon
Keyword(s):  
Aqueous Extract ◽  
Intestinal Parasites ◽  
Vitro Effect

In vitro effect of purified plumbagin of Plumbago indica against motility of Paramphistomum cervi

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
N Saowakon ◽  
P Kueakhai ◽  
N Changklungmoa ◽  
N Lorsuwannarat ◽  
P Sobhon
Keyword(s):  
Plumbago Indica ◽  
Vitro Effect ◽  
Paramphistomum Cervi
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