scholarly journals EFFECTS OF ANION SUBSTITUTIONS ON POTASSIUM-INDUCED PHASIC AND TONIC CONTRACTION IN TAENIA COLI

1967 ◽  
Vol 17 (2) ◽  
pp. 258-266 ◽  
Author(s):  
NORIMOTO URAKAWA ◽  
HIDEAKI KARAKI ◽  
MIYOSHI IKEDA
Keyword(s):  
Tonic Contraction ◽  
Taenia Coli ◽  
Phasic And Tonic Contraction

Discrimination by nimodipine, but not by nifedipine, between phasic and tonic contractions of guinea-pig taenia coli induced by K+ depolarization

1995 ◽  
Vol 73 (11) ◽  
pp. 1600-1604 ◽  
Author(s):  
Sadaharu Usune ◽  
Takeshi Katsuragi ◽  
Tatsuo Furukawa
Keyword(s):  
Guinea Pig ◽  
Tonic Contraction ◽  
Channel Blockers ◽  
Dependent Manner ◽  
Taenia Coli ◽  
Concentration Dependent Manner ◽  
Phasic Contraction ◽  
High K ◽  
Phasic And Tonic Contraction ◽  
Longitudinal Muscles

Effects of Ca2+ channel blockers, such as nifedipine, nimodipine, gallopamil, verapamil, diltiazem, loperamide, Mn2+ and Ni2+, and papaverine, on contractile responses to K+ depolarization were evaluated in longitudinal muscles of taenia coli isolated from guinea-pig. Depolarization with high K+ solution (K+, 40 mM) produced a biphasic (phasic and tonic) contraction, which was inhibited by the above blockers in a concentration-dependent manner. Ratios of IC50 for the phasic contraction to IC50 for the tonic contraction of nimodipine, verapamil, gallopamil, nifedipine, loperamide, diltiazem, papaverine, Ni2+, and Mn2+ were 516.1, 73.7, 22.0, 6.4, 5.3, 4.9, 1.2, 0.7, and 0.1, respectively, indicating that nimodipine suppressed the tonic contraction more effectively than the phasic contraction. In a fluorescence study with fura 2, K+ depolarization elicited an increase in intracellular free Ca2+, [Ca2+]i, which was coupled with the phasic and tonic contraction. The increases in [Ca2+]i coupled with both types of the contraction were abolished by exposure to Ca2+-free solution. In addition, the increase of [Ca2+]i coupled with the phasic contraction was abolished by nifedipine, 10−7 M, but not by nimodipine, 10−7 M, whereas the increase with the tonic contraction was suppressed by both nifedipine and nimodipine. These findings suggest that the phasic and tonic contractions evoked by K+ depolarization are due to increases in [Ca2+]i via activation of respective nimodipine-resistant and nimodipine-sensitive Ca2+ channels in the longitudinal muscles of the taenia coli. Accordingly, nimodipine, but not nifedipine, appears to be a useful tool for distinguishing between the phasic and tonic contractions.Key words: guinea-pig taenia coli, phasic contraction, tonic contraction, nimodipine, nifedipine, intracellular free Ca2+.

Ca45 uptake and tissue calcium in K-induced phasic and tonic contraction in taenia coli

1964 ◽  
Vol 207 (4) ◽  
pp. 873-876 ◽  
Author(s):  
N. Urakawa ◽  
W. C. Holland
Keyword(s):  
Guinea Pig ◽  
Muscle Contraction ◽  
Significant Rise ◽  
Tonic Contraction ◽  
Taenia Coli ◽  
Free Medium ◽  
Phasic Contraction ◽  
High K ◽  
Tissue Calcium ◽  
Phasic And Tonic Contraction

High-K medium produces a tonic contraction in guinea pig taenia coli. If muscle strips are preincubated in glucose-free medium, K produces only a phasic contraction. A comparison of Ca45 entry and tissue Ca changes in the two responses were made. Both responses are accompanied by an enhanced uptake of Ca45. In addition to an increased Ca45 uptake, a significant rise of tissue Ca was observed during the tonic contraction. No detectable changes in tissue Ca were noted in the phasic contraction. In light of modern theories of muscle contraction, it was proposed that in the phasic contraction, sufficient Ca is released from a cellular site to initiate contraction, whereas in the tonic contraction enough Ca crosses the membrane to initiate contraction. The transmembrane Ca transport involved in the latter response appeared to be dependent on metabolism.

scholarly journals Inhibitory effect of beauvericin on a high K+-induced tonic contraction in guinea-pig taenia coli.

1987 ◽  
Vol 45 (3) ◽  
pp. 317-325 ◽  
Author(s):  
Shinjiro NAKAJYO ◽  
Kiyomi MATSUOKA ◽  
Tomohiro KITAYAMA ◽  
Yutaka YAMAMURA ◽  
Kazumasa SHIMIZU ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inhibitory Effect ◽  
Tonic Contraction ◽  
Taenia Coli ◽  
High K

Effects of atriopeptin III on isolated mesenteric resistance vessels from SHR and WKY

1987 ◽  
Vol 253 (6) ◽  
pp. H1612-H1617
Author(s):  
C. Cauvin ◽  
M. Tejerina ◽  
C. van Breemen
Keyword(s):  
Potent Inhibitor ◽  
Tonic Contraction ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Resistance Vessels ◽  
Vascular Beds ◽  
Wistar Kyoto ◽  
Tonic Tension ◽  
Phasic And Tonic Contraction ◽  
Normotensive Control

The effects of atriopeptin III (AP III) were determined on agonist-induced [i.e., 10(-4) M norepinephrine (NE)] and depolarization-induced (80 mM K+) contractions of isolated mesenteric resistance vessels (ID approximately 100 microns) from spontaneously hypertensive rats (SHR) and from normotensive control Wistar-Kyoto (WKY) rats. The vessels from both groups, when activated by 80 mM K+, were unaffected by AP III. However, activation of WKY vessels by 10(-4) M NE (both phasic and tonic contraction) was inhibited quite effectively and potently by AP III, whereas that in SHR vessels was much less inhibited. In the WKY rat vessels, the concentration of AP III that inhibited contraction by 50% for NE-induced phasic tension was 3.1 +/- 1.3 nM, whereas in SHR vessels it was nearly 1 microM. Comparison of AP III inhibition of NE-induced phasic tension to that at 5 min of activation (tonic tension) indicated that the tonic contractions were less sensitive to AP III than the phasic contractions in the vessels from both strains. A similar experiment indicated that AP III was a potent inhibitor of agonist-induced activation in a human renal resistance vessel (ID 125 microns) and that this vessel depended virtually completely on extracellular Ca2+ for NE-induced contraction. These studies contrast with earlier reports (1, 30) that similar peptides inhibited tension only in rat renal resistance vessels and not in resistance vessels from other vascular beds. The decreased sensitivity and efficacy of AP III in inhibiting tension in SHR compared with WKY mesenteric resistance vessels is discussed in the context of the etiology of spontaneous hypertension.

scholarly journals Inhibitory Effect of Beauvericin on a High K+-lnduced Tonic Contraction in Guinea-Pig Taenia Coli

1987 ◽  
Vol 45 (3) ◽  
pp. 317-325
Author(s):  
Shinjiro NAKAJYO ◽  
Kiyomi MATSUOKA ◽  
Tomohiro KITAYAMA ◽  
Yutaka YAMAMURA ◽  
Kazumasa SHIMIZU ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inhibitory Effect ◽  
Tonic Contraction ◽  
Taenia Coli ◽  
High K

Cross-bridge kinetics during shortening in early and sustained contraction of intestinal smooth muscle

1993 ◽  
Vol 265 (3) ◽  
pp. C695-C703 ◽  
Author(s):  
P. Hellstrand ◽  
I. Nordstrom
Keyword(s):  
Smooth Muscle ◽  
Rate Constant ◽  
Time Course ◽  
Tonic Contraction ◽  
Shortening Velocity ◽  
High K ◽  
Cross Bridges ◽  
Latch State ◽  
Slack Length ◽  
Phasic And Tonic Contraction

Mechanisms responsible for the decrease in shortening velocity after prolonged contraction ("latch" state) were investigated at identical force during early (20 s, "phasic") and sustained (5 min, "tonic") phases of high-K+ (25-30 mM) contractions in smooth muscle of guinea pig taenia coli. Cytoplasmic Ca2+ concentration, myosin light-chain phosphorylation, and maximum shortening velocity all declined from 20 s to 5 min of contraction. The time course of shortening following isotonic quick release was biexponential, with a fastest rate constant of approximately 80 s-1 in both phasic and tonic contractions. Stiffness was identical in phasic and tonic contraction; however, after a release to slack length and unloaded shortening, stiffness during restretch was greater in tonic contraction (51 vs. 43% of isometric stiffness after 16 ms of unloaded shortening). Stiffness decreased after release with a rate constant of approximately 200 s-1, slightly greater in phasic than in tonic contraction. The results indicate that the number of attached cross bridges during unloaded shortening, while substantially reduced relative to the isometric value, is higher in latch than in nonlatch, consistent with a lower detachment relative to attachment rate.

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