scholarly journals ELECTRICAL RESPONSE OF GUINEA-PIG SEMINAL VESICLE TO HYPOGASTRIC NERVE STIMULATION IN VIVO AND EFFECT OF RESERPINE

1965 ◽  
Vol 15 (2) ◽  
pp. 199-200 ◽  
Author(s):  
HIRONORI NAKANISHI ◽  
HIROSHI TAKEDA
Keyword(s):  
Guinea Pig ◽  
Seminal Vesicle ◽  
Nerve Stimulation ◽  
Electrical Response ◽  
Hypogastric Nerve

Airway neutral endopeptidase-like enzyme modulates tachykinin-induced bronchoconstriction in vivo

1988 ◽  
Vol 65 (6) ◽  
pp. 2585-2591 ◽  
Author(s):  
D. J. Dusser ◽  
E. Umeno ◽  
P. D. Graf ◽  
T. Djokic ◽  
D. B. Borson ◽  
...  
Keyword(s):  
Substance P ◽  
Guinea Pig ◽  
Vagus Nerve ◽  
Nerve Stimulation ◽  
Guinea Pigs ◽  
Vagus Nerve Stimulation ◽  
Neutral Endopeptidase ◽  
Base Line ◽  
Pulmonary Resistance

To determine whether neutral endopeptidase (NEP), also called enkephalinase (EC 3.4.24.11), modulates the effects of exogenous and endogenous tachykinins in vivo, we studied the effects of aerosolized phosphoramidon, a specific NEP inhibitor, on the responses to aerosolized substance P (SP) and on the atropine-resistant response to vagus nerve stimulation (10 V, 5 ms for 20 s) in guinea pigs. SP alone (10(-7) to 10(-4) M; each concentration, 7 breaths) caused no change in total pulmonary resistance (RL, P greater than 0.5). Phosphoramidon (10(-4) M, 90 breaths) caused no change either in base-line RL (P greater than 0.5) or in the response to aerosolized acetylcholine (P greater than 0.5). However, in the presence of phosphoramidon, SP (7 breaths) produced a concentration-dependent increase in RL at concentrations greater than or equal to 10(-5) M (P less than 0.001). Phosphoramidon (10(-7) to 10(-4) M; each concentration, 90 breaths) induced a concentration-dependent potentiation of SP-induced bronchoconstriction (10(-4) M, 7 breaths; P less than 0.01). Vagus nerve stimulation (0.5-3 Hz), in the presence of atropine, induced a frequency-dependent increase in RL (P less than 0.001). Phosphoramidon potentiated the atropine-resistant responses to vagus nerve stimulation (P less than 0.001) at frequencies greater than 0.5 Hz. The tachykinin antagonist [D-Arg1,D-Pro2,D-Trp7,9,Leu11]-substance P abolished the effects of phosphoramidon on the atropine-resistant response to vagus nerve stimulation (2 Hz, P less than 0.005). NEP-like activity in tracheal homogenates of guinea pig was inhibited by phosphoramidon with a concentration producing 50% inhibition of 5.3 +/- 0.8 nM.(ABSTRACT TRUNCATED AT 250 WORDS)

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