scholarly journals RECS1 is a Negative Regulator of Matrix Metalloproteinase-9 Production and Aged RECS1 Knockout Mice are Prone to Aortic Dilation

2006 ◽  
Vol 70 (5) ◽  
pp. 615-624 ◽  
Author(s):  
Hanjun Zhao ◽  
Akihiko Ito ◽  
Naohiko Sakai ◽  
Yuji Matsuzawa ◽  
Shizuya Yamashita ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Knockout Mice ◽  
Matrix Metalloproteinase 9 ◽  
Negative Regulator ◽  
Aortic Dilation ◽  
Metalloproteinase 9

scholarly journals Epigallocatechin-3-Gallate Inhibits Matrix Metalloproteinase-9 and Monocyte Chemotactic Protein-1 Expression Through the 67-κDa Laminin Receptor and the TLR4/MAPK/NF-κB Signalling Pathway in Lipopolysaccharide-Induced Macrophages

2017 ◽  
Vol 43 (3) ◽  
pp. 926-936 ◽  
Author(s):  
Ya-Fei Li ◽  
Hao Wang ◽  
Yi Fan ◽  
Hao-jie Shi ◽  
Qi-Ming Wang ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Signalling Pathway ◽  
Negative Regulator ◽  
Laminin Receptor ◽  
Mitogen Activated Protein Kinase ◽  
Plaque Instability ◽  
Monocyte Chemotactic Protein ◽  
Chemotactic Protein ◽  
Metalloproteinase 9

Background/Aims: Epigallocatechin-3-gallate (EGCG), a major catechin found in green tea, has been shown to prevent cardiovascular diseases. Previously, Matrix metalloproteinase-9 (MMP-9), monocyte chemotactic protein-1 (MCP-1) and toll-like receptor 4 (TLR4) were confirmed to play an important role in atherosclerosis and plaque instability. Both TLR4 and its negative regulator, Toll-interacting protein (Tollip), could be mediated by EGCG. The present study aimed to examine the effect of physiological concentration of EGCG (1 µM) on the expression of MMP-9 and MCP-1 in lipopolysaccharide (LPS)-induced macrophages and the potential mechanisms underlying its actions. Methods: The RAW264.7 cell line was used. Western blot was used to determine MCP-1, TLR4, Tollip, Mitogen-activated protein kinase (MAPK) and Nuclear factor-κB (NF-κB) protein expression. MMP-9 activity was assayed by gelatine zymography. The mRNA expression of MMP-9 and MCP-1 was measured by realtime polymerase chain reaction (RT-PCR). Results: EGCG (1 µM) significantly suppressed the expression of MMP-9 and MCP-1 and inhibited MAPK and NF-κB in LPS-induced macrophages but was blocked by Tollip silencing. The expression of LPS-induced MMP-9 and MCP-1 and the phosphorylation of the ERK1/2, P38 and NF-κB pathway proteins decreased after TLR4 siRNA treatment. Furthermore, EGCG mediated TLR4 and Tollip expression through binding to 67-kDa laminin receptor (67LR). Conclusion: The results of our study suggested that EGCG (1 µM) suppresses the TLR4/MAPK/NF-κB signalling pathway, decreases the expression of the plaque instability-mediating cytokines MMP-9 and MCP-1, and might prove to be effective in stabilizing atherosclerotic plaque.

473 Adenosine stimulates matrix metalloproteinase-9 secretion by THP-1-derived macrophages

2007 ◽  
Vol 6 (1) ◽  
pp. 102-102
Author(s):  
E VELOT ◽  
I ERNENS ◽  
B HAAS ◽  
C JEANTY ◽  
D ROUY ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Matrix Metalloproteinase 9 ◽  
Metalloproteinase 9
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