scholarly journals Effect of liquid flow on culture of red beet hairy roots in single column reactor.

1997 ◽  
Vol 30 (6) ◽  
pp. 1070-1075 ◽  
Author(s):  
Yoshio Hitaka ◽  
Masahiro Kino-oka ◽  
Masahito Taya ◽  
Setsuji Tone
Keyword(s):  
Hairy Roots ◽  
Liquid Flow ◽  
Column Reactor ◽  
Red Beet ◽  
Single Column

Effect of Liquid Flow on Pigment Formation of Red Beet Hairy Roots.

1999 ◽  
Vol 32 (3) ◽  
pp. 370-373 ◽  
Author(s):  
Yoshio Hitaka ◽  
Masahiro Kino-oka ◽  
Masahito Taya ◽  
Setsuji Tone
Keyword(s):  
Hairy Roots ◽  
Liquid Flow ◽  
Pigment Formation ◽  
Red Beet

Batch and Fed-Batch Production of Betalains by Red Beet (Beta vulgaris) Hairy Roots in a Bubble Column Reactor

2007 ◽  
Vol 62 (5-6) ◽  
pp. 439-446 ◽  
Author(s):  
Atanas Pavlov ◽  
Milen Georgiev ◽  
Thomas Bley
Keyword(s):  
Beta Vulgaris ◽  
Hairy Roots ◽  
Bubble Column ◽  
Dry Weight ◽  
Bubble Column Reactor ◽  
Fed Batch ◽  
Column Reactor ◽  
Red Beet ◽  
Air Supply ◽  
Dry Biomass

Hairy root cultures from red beet (Beta vulgaris L.), which could be used for the commercial production of biologically active betalain pigments, were cultivated in a 3 L bubble column bioreactor in batch mode with various rates of air supply. Both the growth of the roots and betalain volumetric yields were highest (12.7 g accumulated dry biomass/L and 330.5 mg/ L, respectively) with a 10 L/h (0.083 vvm) air supply. The air flow rate also influenced the betacyanins/betaxanthins ratios in the cultures. Growth and betalains production were then examined in two fed-batch regimes (with a 10 L/h air supply), in which nutrient medium was fed just once or on five occasions, designated FBI and FBII, respectively. The root mass accumulation was increased in the FBI feeding regime (to 13.3 g accumulated dry biomass/ L), while in FBII the betalains content was ca. 11% higher (15.1 mg betacyanins/g dry weight and 14.0 mg betaxanthins/g dry weight) than in the most productive batch regime. Data on the time course of the utilization of major components in the medium during both operational modes were also collected. The implications of the information acquired are discussed, and the performance of the hairy roots (in terms of both growth and betalains production) in the bubble column reactor and previously investigated cultivation systems is compared.

Evaluation of growth property of red beet hairy roots depending on condition of inocula and its application to culture control with fuzzy logic theory

2001 ◽  
Vol 8 (2) ◽  
pp. 121-127 ◽  
Author(s):  
Yusuke Takahashi ◽  
Yoshio Hitaka ◽  
Masahiro Kino-oka ◽  
Masahito Taya ◽  
Setsuji Tone
Keyword(s):  
Fuzzy Logic ◽  
Hairy Roots ◽  
Growth Property ◽  
Red Beet ◽  
Fuzzy Logic Theory

Peroxidases and Other Enzymes from Red Beet Hairy Roots

2012 ◽  
pp. 283-333 ◽  
Author(s):  
Bhagyalakshmi Neelwarne ◽  
Thimmaraju Rudrappa
Keyword(s):  
Hairy Roots ◽  
Red Beet

scholarly journals Decaffeination process characteristic of Robusta coffee in single column reactor using ethyl acetate solvent

2009 ◽  
Vol 25 (2) ◽  
Author(s):  
Sukrisno Widyotomo ◽  
Sri Mulato ◽  
Hadi K. Purwadaria ◽  
A.M Syarief
Keyword(s):  
Ethyl Acetate ◽  
Product Diversification ◽  
Column Reactor ◽  
Market Expansion ◽  
Robusta Coffee ◽  
Caffeine Content ◽  
Coffee Beans ◽  
Single Column ◽  
Process Characteristic ◽  
Bean Size

Consumers drink coffee not as nutrition source, but as refreshment drink. For coffee consumers who have high tolerance for caffeine, coffee may warm up and refresh their bodies. High caffeine content in coffee beans may cause several complaints to consumers who are susceptible to caffeine. One of the efforts, for coffee market expansion is product diversification to decaffeinated coffee. Decaffeination process is one of process to reduce caffeine content from agricultural products. Indonesian Coffee and Cocoa Research Institute in collaboration with Bogor Agricultural University has developed a single column reactor for coffee beans decaffeination. The aim of this research is to study process characteristic of coffee decaffeination in single column reactor using ethyl acetate (C4H8O2) solvent. Treatments applicated in the research were time and temperature process. Temperature treatment were 50—60OC, 60—70OC, 70—80OC, 80—90OC and 90—100OC. Time treatment were 2 h, 4 h, 6 h, 8 h, 10 h, and 12 h Size of Robusta coffee beans used were less than 5.5 mm (A4), between 5.5 mm and 6.5 mm (A3), between 6.5 mm and 7.5 mm (A2), and more than 7.5 mm (A1). The result showed that decaffeination process with ethyl acetate solvent will be faster when its temperature was higher and smaller bean size. For bean size less than 5,5 mm, decaffeination process by 10% ethyl acetat can be done 8—10 hours in 90—100OC solvent temperature or 12 hours in 60—70OC solvent temperature for 0.3% caffein content. Organoleptic test showed that 90—100OC temperature solvent treatment decreased coffee flavor, which aroma, bitterness and body values were 1.9 each . Key words : Coffee, caffeine, decaffeination, quality, single column.

Bioreactors for the Cultivation of Red Beet Hairy Roots

2012 ◽  
pp. 251-281 ◽  
Author(s):  
Vasil G. Georgiev ◽  
Thomas Bley ◽  
Atanas I. Pavlov
Keyword(s):  
Hairy Roots ◽  
Red Beet

Food-Grade Chemical and Biological Agents Permeabilize Red Beet Hairy Roots, Assisting the Release of Betalaines

2008 ◽  
Vol 19 (4) ◽  
pp. 1274-1282 ◽  
Author(s):  
R. Thimmaraju ◽  
N. Bhagyalakshmi ◽  
M. S. Narayan ◽  
G. A. Ravishankar
Keyword(s):  
Hairy Roots ◽  
Biological Agents ◽  
Food Grade ◽  
Red Beet
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