scholarly journals Coffee Induces 11β-Hydroxysteroid Dehydrogenase Type 2 Activity in Human Placental Choriocarcinoma Cells, JEG-3

2011 ◽  
Vol 57 (5) ◽  
pp. 436-441 ◽  
Author(s):  
Tomomi Arao ◽  
Hiroomi Tamura
Keyword(s):  
Hydroxysteroid Dehydrogenase ◽  
Choriocarcinoma Cells

Regulation of 11β-hydroxysteroid dehydrogenase type 2 activity and mRNA in human choriocarcinoma cells

1996 ◽  
Vol 16 (3) ◽  
pp. 269-275 ◽  
Author(s):  
M M Pasquarette ◽  
P M Stewart ◽  
M L Ricketts ◽  
K Imaishi ◽  
J I Mason
Keyword(s):  
Protein Kinase ◽  
Northern Blot Analysis ◽  
Hydroxysteroid Dehydrogenase ◽  
Dependent Manner ◽  
Human Trophoblast ◽  
Kinase C ◽  
Cytotrophoblastic Cell ◽  
Choriocarcinoma Cells ◽  
Human Choriocarcinoma Cells

ABSTRACT The type 2 isoform of 11 β-hydroxysteroid dehydrogenase (11β-HSD2), which catalyzes the conversion of cortisol to hormonally inactive cortisone in man, is principally expressed in the placenta and mineralocorticoid target tissues, kidney and colon. To date, few studies have addressed the regulation of this novel 11β-HSD2 isoform. We have characterized the nature and regulation of the 11β-HSD activity expressed in a human cytotrophoblastic cell line, the JEG-3 choriocarcinoma cell. The 11β-HSD activity in JEG-3 cell homogenates required NAD+ as cofactor with NADP ineffective and demonstrated a high affinity for cortisol (apparent Km 31 nm). Incubation of JEG-3 cells with forskolin and dibutyryl cyclic AMP increased 11β-HSD2 activity several-fold in a time-dependent manner, while treatment with phorbol ester had little, if any, effect on 11β-HSD2 activity. Northern blot analysis of RNA isolated from JEG-3 cells after these treatments demonstrated a marked increase in a 1·9 kb 11β-HSD2 mRNA species in cells treated with forskolin for 24 h. We conclude that 11β-HSD2 is regulated by activation of the protein kinase A pathway, but not the protein kinase C pathway in human choriocarcinoma cells, and that this regulation occurs at a pretranslational level. JEG-3 cells provide an excellent model for further studies on the regulation of 11β-HSD2 gene expression in human trophoblast tissue.

scholarly journals Synthesis, purification and crystallographic studies of the C-terminal sterol carrier protein type 2 (SCP-2) domain of human hydroxysteroid dehydrogenase-like protein 2

2015 ◽  
Vol 71 (7) ◽  
pp. 901-905 ◽  
Author(s):  
Zhong Cheng ◽  
Yao Li ◽  
Chun Sui ◽  
Xiaobo Sun ◽  
Yong Xie
Keyword(s):  
Catalytic Domain ◽  
Hydroxysteroid Dehydrogenase ◽  
Carrier Protein ◽  
Sterol Carrier Protein ◽  
X Ray Diffraction ◽  
Unit Cell Parameters ◽  
X Ray ◽  
Cell Parameters ◽  
Protein Molecules

Human hydroxysteroid dehydrogenase-like protein 2 (HSDL2) is a member of the short-chain dehydrogenase/reductase (SDR) subfamily of oxidoreductases and contains an N-terminal catalytic domain and a C-termianl sterol carrier protein type 2 (SCP-2) domain. In this study, the C-terminal SCP-2 domain of human HSDL2, including residues Lys318–Arg416, was produced inEscherichia coli, purified and crystallized. X-ray diffraction data were collected to 2.10 Å resolution. The crystal belonged to the trigonal space groupP3121 (orP3221), with unit-cell parametersa=b= 70.4,c= 60.6 Å, α = β = 90, γ = 120°. Two protein molecules are present in the asymmetric unit, resulting in a Matthews coefficient of 2.16 Å3 Da−1and an approximate solvent content of 43%.

11β Hydroxysteroid Dehydrogenase Type 2 in the Adrenal Gland by Testosterone Withdrawal of Adult Rats

Folia Medica ◽  
2010 ◽  
Vol 52 (2) ◽  
Author(s):  
Yvetta Koeva ◽  
Mariana Bakalska ◽  
Elisaveta Petrova ◽  
Nina Atanassova
Keyword(s):  
Adrenal Gland ◽  
Hydroxysteroid Dehydrogenase ◽  
Adult Rats

11β hydroxysteroid dehydrogenase type 2 enzyme is expressed in normotensive and hypertensive patients with renal disease

Nephrology ◽  
2008 ◽  
Vol 4 (1-2) ◽  
pp. 81-86
Author(s):  
Alicia N STEIN-OAKLEY ◽  
Julie A MAGUIRE ◽  
John DOWLING ◽  
Greg J PERRY ◽  
Zygmunt KROZOWSKI ◽  
...  
Keyword(s):  
Renal Disease ◽  
Hydroxysteroid Dehydrogenase ◽  
Hypertensive Patients

scholarly journals 11?-Hydroxysteroid dehydrogenase Type 1 in obesity and Type 2 diabetes

Diabetologia ◽  
2004 ◽  
Vol 47 (1) ◽  
pp. 1-11 ◽  
Author(s):  
T. M. Stulnig ◽  
W. Waldh�usl
Keyword(s):  
Type 2 Diabetes ◽  
Hydroxysteroid Dehydrogenase

scholarly journals Two Elderly Patients with Mineralocorticoid Excess Due to 11β-Hydroxysteroid Dehydrogenase Type 2 (11β-HSD2) Impairment

2008 ◽  
Vol 47 (7) ◽  
pp. 631-636 ◽  
Author(s):  
Mitsuo Inada ◽  
Keiko Iwasaki ◽  
Chihiro Imai ◽  
Satoshi Hashimoto
Keyword(s):  
Elderly Patients ◽  
Hydroxysteroid Dehydrogenase

scholarly journals Fourth-Generation Progestins Inhibit 3β-Hydroxysteroid Dehydrogenase Type 2 and Modulate the Biosynthesis of Endogenous Steroids

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164170 ◽  
Author(s):  
Renate Louw-du Toit ◽  
Meghan S. Perkins ◽  
Jacky L. Snoep ◽  
Karl-Heinz Storbeck ◽  
Donita Africander
Keyword(s):  
Hydroxysteroid Dehydrogenase ◽  
Fourth Generation ◽  
Endogenous Steroids
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