scholarly journals Rapid Identification and Enumeration of Antibiotic Resistant Bacteria in Urban Canals by Microcolony-Fluorescence in Situ Hybridization

2006 ◽  
Vol 52 (6) ◽  
pp. 703-710 ◽  
Author(s):  
Takehiko Kenzaka ◽  
Nobuyasu Yamaguchi ◽  
Fuangfa Utrarachkij ◽  
Orasa Suthienkul ◽  
Masao Nasu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Rapid Identification ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant

Fibrous testing papers for fluorescence trace sensing and photodynamic destruction of antibiotic-resistant bacteria

2020 ◽  
Vol 8 (13) ◽  
pp. 2709-2718 ◽  
Author(s):  
Long Zhao ◽  
Yuan Liu ◽  
Zhanlin Zhang ◽  
Jiaojun Wei ◽  
Songzhi Xie ◽  
...  
Keyword(s):  
Rapid Identification ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant

The increasing prevalence of antibiotic-resistant bacteria needs rapid identification and efficient destruction routes.

scholarly journals DNA Mimics for the Rapid Identification of Microorganisms by Fluorescence in situ Hybridization (FISH)

2008 ◽  
Vol 9 (10) ◽  
pp. 1944-1960 ◽  
Author(s):  
Laura Cerqueira ◽  
Nuno Azevedo ◽  
Carina Almeida ◽  
Tatiana Jardim ◽  
Charles Keevil ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Rapid Identification

scholarly journals Detection of Ampicillin-Resistant E. coli Using Novel Nanoprobe-Combined Fluorescence In Situ Hybridization

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 750 ◽  
Author(s):  
Wang Sik Lee ◽  
Soohyun Lee ◽  
Taejoon Kang ◽  
Choong-Min Ryu ◽  
Jinyoung Jeong
Keyword(s):  
Antibiotic Resistance ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Antibiotic Resistance Gene ◽  
Sol Gel ◽  
Light Condition ◽  
Resistant Bacteria ◽  
Specific Gene ◽  
E Coli

Antibiotic-resistant bacteria present a global threat because the infections they cause are difficult to treat. Therefore, it is highly important to develop advanced methods for the identification of antibiotic resistance gene in the virulent bacteria. Here, we report the development of novel nanoprobes for fluorescence in situ hybridization (FISH) and the application of the nanoprobe to the detection of ampicillin-resistant Escherichia coli. The nanoprobe for FISH was synthesized by the modified sol–gel chemistry and the synthesized nanoprobe provided strong fluorescent signals and pH stability even under natural light condition. For the double-identification of bacteria species and ampicillin-resistance with a single probe in situ, the nanoprobes were conjugated to the two kinds of biotinylated probe DNAs; one for E. coli-species specific gene and the other for a drug-resistant gene. By using the nanoprobe-DNA conjugants, we successfully detected the ampicillin-resistant E. coli through the FISH technique. This result suggests the new insight into light stable FISH application of the nanoprobe for a pathogenic antibiotic-resistance bacterium.

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