scholarly journals Ion-Pair Reversed-Phase HPLC Method for Determination of Sodium Tanshinone IIA Sulfonate in Biological Samples and Its Pharmacokinetics and Biodistribution in Mice

2007 ◽  
Vol 55 (5) ◽  
pp. 753-756 ◽  
Author(s):  
ShengJun Mao ◽  
Hui Jin ◽  
YueQi Bi ◽  
Zhen Liang ◽  
Hui Li ◽  
...  
Keyword(s):  
Biological Samples ◽  
Reversed Phase ◽  
Hplc Method ◽  
Ion Pair ◽  
Tanshinone Iia ◽  
Reversed Phase Hplc ◽  
Sodium Tanshinone Iia Sulfonate

Ion-pair reversed-phase HPLC method for the identification and determination of phosphothiamine in cocarboxylase hydrochloride

1997 ◽  
Vol 31 (5) ◽  
pp. 274-275
Author(s):  
S. V. Kuz'min ◽  
E. E. Ishina ◽  
M. L. Shishova ◽  
V. A. Kozlov
Keyword(s):  
Reversed Phase ◽  
Hplc Method ◽  
Ion Pair ◽  
Reversed Phase Hplc

An ion-pair reversed-phase HPLC method for determination of fresh tissue adenine nucleotides avoiding freeze–thaw degradation of ATP

2009 ◽  
Vol 388 (1) ◽  
pp. 108-114 ◽  
Author(s):  
Stephanie zur Nedden ◽  
Robert Eason ◽  
Alexander S. Doney ◽  
Bruno G. Frenguelli
Keyword(s):  
Adenine Nucleotides ◽  
Reversed Phase ◽  
Hplc Method ◽  
Ion Pair ◽  
Reversed Phase Hplc ◽  
Fresh Tissue ◽  
Freeze Thaw

Determination of 5-hydroxymethyl-2-furaldehyde in royal jelly by a rapid reversed phase HPLC method

2013 ◽  
Vol 5 (19) ◽  
pp. 5010 ◽  
Author(s):  
Marco Ciulu ◽  
Roberta Farre ◽  
Ignazio Floris ◽  
Valeria M. Nurchi ◽  
Angelo Panzanelli ◽  
...  
Keyword(s):  
Royal Jelly ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc

scholarly journals Simultaneous Determination of Preservatives (Methyl Paraben and Propyl Paraben) in Sucralfate Suspension Using High Performance Liquid Chromatography

2011 ◽  
Vol 8 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Rajesh M. Kashid ◽  
Santosh G. Singh ◽  
Shrawan Singh
Keyword(s):  
High Performance Liquid Chromatography ◽  
Simultaneous Determination ◽  
Mobile Phase ◽  
High Performance ◽  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc ◽  
Methyl Paraben ◽  
Propyl Paraben

A reversed phase HPLC method that allows the separation and simultaneous determination of the preservatives methyl paraben (M.P.) and propyl paraben (P.P.) is described. The separations were effected by using an initial mobile phase of water: acetonitrile (50:50) on Inertsil C18 to elute P.P. and M.P. The detector wavelength was set at 205 nm. Under these conditions, separation of the two components was achieved in less than 10 min. Analytical characteristics of the separation such as precision, specificity, linear range and reproducibility were evaluated. The developed method was applied for the determination of preservative M.P. and P.P. at concentration of 0.01 mg/mL and 0.1 mg/mL respectively. The method was successfully used for determining both compounds in sucralfate suspension.

A New Reversed Phase HPLC Method for the Determination of Spiramycins I, II and III

1998 ◽  
Vol 11 (3) ◽  
pp. 167-171 ◽  
Author(s):  
Shabana I. Khan ◽  
David C. Limburg ◽  
Iklas A. Khan ◽  
John S. Williamson
Keyword(s):  
Reversed Phase ◽  
Hplc Method ◽  
Reversed Phase Hplc

scholarly journals Improved HPLC Conditions to Determine Eumelanin and Pheomelanin Contents in Biological Samples Using an Ion Pair Reagent

2020 ◽  
Vol 21 (14) ◽  
pp. 5134 ◽  
Author(s):  
Shosuke Ito ◽  
Sandra Del Bino ◽  
Tomohisa Hirobe ◽  
Kazumasa Wakamatsu
Keyword(s):  
Dicarboxylic Acid ◽  
Biological Samples ◽  
Potassium Phosphate ◽  
Structural Diversity ◽  
Tricarboxylic Acid ◽  
Reversed Phase ◽  
Hplc Method ◽  
Original Method ◽  
Ion Pair ◽  
Improved Method

Alkaline hydrogen peroxide oxidation (AHPO) of eumelanin and pheomelanin, two major classes of melanin pigments, affords pyrrole-2,3,5-tricarboxylic acid (PTCA), pyrrole-2,3-dicarboxylic acid (PDCA) and pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA) from eumelanin and thiazole-2,4,5-tricarboxylic acid (TTCA) and thiazole-4,5-dicarboxylic acid (TDCA) from pheomelanin. Quantification of these five markers by HPLC provides useful information on the quantity and structural diversity of melanins in various biological samples. HPLC analysis of these markers using the original method of 0.1 M potassium phosphate buffer (pH 2.1):methanol = 99:1 (85:15 for PTeCA) on a reversed-phase column had some problems, including the short lifetime of the column and, except for the major eumelanin marker PTCA, other markers were occasionally overlapped by interfering peaks in samples containing only trace levels of these markers. These problems can be overcome by the addition of an ion pair reagent for anions, such as tetra-n-butylammonium bromide (1 mM), to retard the elution of di-, tri- and tetra-carboxylic acids. The methanol concentration was increased to 17% (30% for PTeCA) and the linearity, reproducibility, and recovery of the markers with this improved method is good to excellent. This improved HPLC method was compared to the original method using synthetic melanins, mouse hair, human hair, and human epidermal samples. In addition to PTCA, TTCA, a major marker for pheomelanin, showed excellent correlations between both HPLC methods. The other markers showed an attenuation of the interfering peaks with the improved method. We recommend this improved HPLC method for the quantitative analysis of melanin markers following AHPO because of its simplicity, accuracy, and reproducibility.

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